Assessment of genetic relationships among Anthurium cultivars using nucleotide sequence of chitinase and beta-1, 3-glucanase genes

บทคัดย่อ

Chitinase and -1, 3-glucanase are members of pathogenesis-related proteins (PR proteins) that are strongly induced when host plant cells are faced by pathogen stress. Thus, chitinase and -1, 3-glucanase constitute an important protein of plants against pathogens. In this study, the genetic relationships of ten Anthurium cultivars were analyzed using nucleotide sequences of chitinase and -1,3-glucanase gene. DNA was extracted and used as a template in the polymerase chain reaction (PCR) using chitinase and -1, 3-glucanase specific primers. The length of chitinase gene sequences of ten Anthurium cultivars ranged from 655 to 722 bp and the average length was 690.70 bp. The length of -1, 3-glucanase gene sequences ranged from 442 to 490 bp and the average length was 473.20 bp. the genetic relationship analysis using Kimura 2-parameter indicated that the genetic similarity index based on chitinase gene sequence has an average of 47.20%. The lowest value (30.17%) was between Red Hot and Sangtien and the highest value (100%) was between Cherry Pink and Red Hot and between Chompoo Noppon and Red Strong. The genetic similarity index based on -1, 3-glucanase gene sequence has an average of 53.50%. The lowest value (32.17%) was between Merengue and Plew Tien Phuket and the highest value (100%) was between Red Bar and Sun Red. The genetic similarity index based on a combination of chitinase and -1, 3-glucanase gene sequence has an average of 49.00%. The lowest value (33.73%) was between Chompoo Noppon and Plew Tien Phuket and the highest value (90.83%) was between Plew Tien Lampang and Sangtien. All dendrograms demonstrated that 10 Anthurium cultivar were divided into two major groups but the clustering was not correlated with morphology. The close relationship between high disease resistance cultivar was found.