Assessment of genetic relationships among Anthurium cultivars using nucleotide sequence of chitinase and beta-1, 3-glucanase genes

  • Nattapong Srisamoot Department of Biotechnology, Faculty of Agricultural Technology, Kalasin University
  • Teamjun Srisamoot Department of Science, Yasothon Pittayakom School, Mueang Yasothon District, Yasothon
  • Chalita Chookanhom Department of Biotechnology, Faculty of Agricultural Technology, Kalasin University
Keywords: Genetic relationship, Anthurium andreanum, chitinase, beta-1, 3-glucanase

Abstract

Chitinase and gif.latex?\beta-1, 3-glucanase are members of pathogenesis-related proteins (PR proteins) that are strongly induced when host plant cells are faced by pathogen stress. Thus, chitinase and gif.latex?\beta-1, 3-glucanase constitute an important protein of plants against pathogens. In this study, the genetic relationships of ten Anthurium cultivars were analyzed using nucleotide sequences of chitinase and gif.latex?\beta-1,3-glucanase gene. DNA was extracted and used as a template in the polymerase chain reaction (PCR) using chitinase and gif.latex?\beta-1, 3-glucanase specific primers. The length of chitinase gene sequences of ten Anthurium cultivars ranged from 655 to 722 bp and the average length was 690.70 bp. The length of gif.latex?\beta-1, 3-glucanase gene sequences ranged from 442 to 490 bp and the average length was 473.20 bp. the genetic relationship analysis using Kimura 2-parameter indicated that the genetic similarity index based on chitinase gene sequence has an average of 47.20%. The lowest value (30.17%) was between Red Hot and Sangtien and the highest value (100%) was between Cherry Pink and Red Hot and between Chompoo Noppon and Red Strong. The genetic similarity index based on gif.latex?\beta-1, 3-glucanase gene sequence has an average of 53.50%. The lowest value (32.17%) was between Merengue and Plew Tien Phuket and the highest value (100%) was between Red Bar and Sun Red. The genetic similarity index based on a combination of chitinase and gif.latex?\beta-1, 3-glucanase gene sequence has an average of 49.00%. The lowest value (33.73%) was between Chompoo Noppon and Plew Tien Phuket and the highest value (90.83%) was between Plew Tien Lampang and Sangtien. All dendrograms demonstrated that 10 Anthurium cultivar were divided into two major groups but the clustering was not correlated with morphology. The close relationship between high disease resistance cultivar was found.

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Published
2020-07-09
How to Cite
Srisamoot, N., Srisamoot, T., & Chookanhom, C. (2020). Assessment of genetic relationships among Anthurium cultivars using nucleotide sequence of chitinase and beta-1, 3-glucanase genes. Journal of Roi Et Rajabhat University: Science and Technology, 1(1), 1-12. Retrieved from https://ph02.tci-thaijo.org/index.php/ScienceRERU/article/view/241753
Section
Research Articles