Study of Hydrolase Activities from Germinated Ascosphaera Apis Spores
doi: 10.14456/mijet.2016.9
Keywords:
Ascosphaera apis, spore germination, amylase, lipase and protease activitiesAbstract
Spores from Ascosphaera apis, a fungus causing chalkbrood disease of honey bees, were cultivated in Sabouraud Dextrose Broth (SDB) at room temperature. Spore germination and hydrolase activities in the liquid medium were detected every 3 hr. of cultivation. It was found that spore germination increased with the cultivation time and reached 32.78±0.51% at the 27th hr. The hydrolase activities increased at the initial stage of germination and then decreased. The maximum activities of amylase, lipase and protease at 6, 9 and 12 hr. of cultivation were 3.76±0.16 U/mL, 3.58±0.70 U/mL and 43.31±0.92 U/mL respectively.
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