Effects of Uvaria rufa Blume on the Histology of Male Reproductive Organs of Testosterone Induced Alopecia Rats
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Published:
Jun 30, 2018
Keywords:
Androgenic alopecia Histology Reproductive organs Testosterone Uvaria rufa Blume
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Abstract
Reports on the male reprotoxicity of some medicinal plants have been documented. This study was undertaken to evaluate the effects of ethyl acetate extract from the stems of Uvaria rufa Blume (UREtA) on the male reproductive organs of alopecia-induced rats. Androgenic alopecia (AP) was induced by testosterone propionate (0.07%). After hormonal injection, the rats were topically given vehicle (AP model), minoxidil, and 2% and 4% UREtA for 21 days. The normal control rats were given olive oil injections and received distilled water. The relative weights of the testes, epididymis, prostate glands, and epididymis, and histopathological alterations were examined. The results showed that testosterone injection reduced the relative weights of all organs when compared to those of the normal control, while significant decreases (P>0.05) were found only in epididymis and prostate glands. The increases in relative weights of epididymis observed in all treated groups, also testes and prostate glands observed in 4% UREtA and minoxidil, including seminal vesicles observed in 2% UREtA were clearly noted when compared to those of AP models. Vacuolizations were observed in testicular tissues treated with both concentrations of UREtA, while germ cell death in the lumens of the seminiferous tubules was seen in the minoxidil treated group. Dead spermatozoa in the lumens of the epididymis were markedly noted in all groups. Moreover, AP-induced rats treated with minoxidil revealed leucocyte infiltrations and blood congestions in their prostatic tissues. Therefore, it can be concluded that 2% and 4% UREtA has some adverse effects on male reproductive organs.
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(1)
Pamok, S. Effects of Uvaria Rufa Blume on the Histology of Male Reproductive Organs of Testosterone Induced Alopecia Rats. Microsc. Microanal. Res. 2018, 31, 9-17.
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References
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16. A. Kassem, A. Al-Aghbari, M. Al-Habori, M. Al-Mamary, Evaluation of the potential antifertility effect of fenugreek seeds in male and female rabbits, Contraception., 2006, 73, 301-306.
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24. K. Deepralard, K. Kawanishi, M. Moriyasu, T. Pengsuparp, R. Suttisi, Flavonoid glycosides from the leaves of Uvaria rufa with advanced glycation end-products inhibitory activity. Thai J. Pharm. Sci., 2009, 33, 84-90.
25. S.S. Roh, C.D. Kim, M.H. Lee, S.L. Hwang, M.J. Rang, Y.K. Yoon, The hair growth promoting effect of Sophora flavescens extract and its molecular regulation. J. Dermatol. Sci., 2002, 30, 43-49.
26. G.A. Ayoola, H.A.B. Coker, S.A. Adesegun, A.A. Adepoju-Bellol, K. Obaweyal, E.C. Ezennial, T.O. Atangbayilal, Phytochemical screening and antioxidant activities of some selected medicinal plants used for malaria therapy in Southwestern
Nigeria, Trop. J. Pharm. Res., 2008, 7, 1019-1024.
27. H.O. Edeoga, D.E. Okwu, B.O. Mbaebie, Phytochemical constituents of some Nigerian medicinal plants. Afr. J. Biotechnol., 2005, 4, 685-688.
28. X.W. Chen, E.S. Serag, K.B. Sneed, S.F. Zhou, Herbal bioactivation, molecular targets and toxicity relevance, Chem. Biol. Interact., 2001, 192, 161-176.
29. Z.F. Dewan, I.D. Morris, R.G. Lendon, Administration of exogenous testosterone in the adult rat and its effects on reproductive organs, sex hormones and body weight, Bangladesh Med. Res. Counc. Bull., 2000, 26, 48-55.
30. L. Tόthová, P. Celec, D. Ostatníková, M. Okuliarová, M. Zeman, J. Hodosy, Effect of exogenous testosterone on oxidative status of the testes in adult male rats. Andrologia., 2013, 45, 417-423.
31. S. Prasad, N. Kalra, Y. Shukla, Modulatory effects of diallyl ulphide against testosterone-induced oxidative stress in Swiss albino mice. Asian J. Androl., 2006, 8, 719-723.
32. L.A.J. Shittu, M.A. Bankole, J.A. Oguntola, O. Ajala, R.K. Shittu, O.A. Ogundipe, M.N. Bankole, T. Ahmed, O.A. Ashiru, Sesame leaves intake improve and increase epididymal spermatocytes reserve in adults male Sprague Dawley rat, Sci. Res. Essays., 2007, 2, 319-324.
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34. R. Mosbah, M.I. Yousef, A. Mantovani, Nicotine-induced reproductive toxicity, oxidative damage, histological changes and haematotoxicity in male rats: the protective effects of green tea extract, Exp. Toxicol. Pathol., 2015, 67, 253-259.
35. M.A. Rezvanfar, R.A. Sadikhanlou, A. Ahmadi, H. Shojaei-Sadee, M.A. Rezvanfar, A. Mohammadirad, A. Salehnia, M. Abdollahi, Protective of cyclophosphamide-induced toxicity in reproductive tract histology, sperm characteristics, and DNA
damage by an herbal source; evidence for role of free-radical toxic stress, Hum. Exp. Toxicol., 2008, 27, 901-910.
36. M. Mohamed, S.A. Sulaiman, H. Jaafar, Histological changes in male accessory reproductive organs in rats exposed to cigarette smoke and the protective effect of honey supplementation, Afr. J. Tradit. Complement. Altern. Med., 2012, 9, 329-335.
37. A. Hentrich, M. Wolter, C. Szardening-Kirchner, G.H. Lüers, M. Bergmann, S. Kliesch, L. Konrad, Reduced numbers of Sertoli, germ, and spermatogonial stem cells in impaired spermatogenesis, Mod. Pathol., 2001, 24, 1380-1389.
38. W. Liao, M. Cai, J. Chen, J. Huang, F. Liu, C. Jiang, Y. Gao, Hypobaric hypoxia causes deleterious effects on spermatogenesis in rats, Reproduction., 2010, 139, 1031-1038.
39. S. Chuma, M. Hosokawa, K. Kitamura, S. Kasai, M. Fujioka, M. Hiyoshi, K. Takamune, T. Noce, N. Nakatsuji, Tdrd1/Mtr-1, a tudor-related gene, is essential for male germ-cell differentiation and nuage/germinal granule formation in mice, Proc. Natl. Acad. Sci. U.S.A., 2006, 103, 15894-15899.
40. D.D. Seachrist, E. Johnson, C. Magee, C.M. Clay, J.K. Graham, D.N. Veeramachaneni, R.V. Keri, Overexpression of follistatin in the mouse epididymis disrupts fluid resorption and sperm transit in testicular excurrent ducts, Biol. Reprod., 2012, 87, 41.
41. Y. Ji, J. Sun, L. Lang, Analysis of testicular toxicity of salanine in mice, Adv. Mater. Res., 2010, 282-283, 195-200.
42. H. Kloer, H. Hoogen, E. Nieschlag, Trial of high-dose testosterone undecanoate in treatment of male infertility, Int. J. Androl., 1980, 3, 121-129.
43. A. M. Matsumoto, Is high dosage testosterone an effective male contraceptive agent?, Fertil. Steril., 1988, 50, 324-328.
44. J.D. Vidal, K.M. Whitney, Morphologic manifestations of testicular and epididymal toxicity, Spermatogenesis., 2014, 4, e979099.
2. V.A. Randall, Androgen and human hair growth, Clin. Endocrinol (Oxf)., 2002, 40, 439-457.
3. K.D. Kaufman, Androgens and alopecia, Mol. Cell Endocrinol., 2002, 198, 89-95.
4. E.A. Olsen, F.E. Dunlap, T. Funicella, J.A. Koperski, J.M. Swinehart, E.H. Tschen, A randomized clinical trial of 5% topical minoxidil versus 2% topical minoxidil and placebo in the treatment of androgenetic alopecia in men. J. Am. Acad. Dermatol., 2002, 47, 377-385.
5. R. Tsuboi, S. Itami, S. Inui, R. Ueki, K. Katsuoka, S. Kurata, Guidelines for the management of androgenetic alopecia, J. Dermatol., 2012, 39, 113-120.
6. S. Varothai, W.F. Bergfeld, Androgenetic alopecia: an evidence-based treatment update, Am. J. Clin. Dermatol., 2014, 15, 217-230.
7. H. Ishida, Y. Inaoka, M. Okada, M. Fukushima, H. Fukazawa, K. Tsuji, Studies of the active substances in herbs used for hair treatment III isolation of hair-regrowth substances from Polygara senega var. latifolia TORR. Et GRAY., Biol. Pharma. Bull., 1999, 22, 1249-1250.
8. P.S. Banerjee, M. Sharma, R.K. Nema, Preparation, evaluation and hair growth stimulating activity of herbal hair oil, J. Chem. Pharma. Res., 2009, 1, 261-267.
9. C.S. Shah, J.S. Qudry, A text book of pharmacognosy, eleven ed., B.S. Shah Prakashan Publisher, Ahmadabad, 1996.
10. M. Semalty, A. Semalty, G.P. Joshi, M.S.M. Rawat, In vivo hair growth activity of herbal formulations, Int. J. Pharmacol., 2010, 6, 53-57.
11. R.K. Roy, M. Thakur, V.K. Dixit, Effect of Citrullus colocynthis on hair growth in albino rats, Pharma. Biol., 2007, 45, 739-744.
12. V.R. Gottumukkala, T. Annamalai, T. Mukhopadhyay, Phytochemical investigation and hair growth studies on the rhizomes of Nardostachys jatamansi DC, Pharmacogn. Mag. 2011, 26, 146-150.
13. K.E. Sharquie, H.K. Al-Obaidi, Onion juice (Allium cepa L.), a new topical treatment for alopecia areata, J. Dermatol., 2002, 29, 343-346.
14. N. Harada, K. Okajima, M. Arai, H. Kurihara, N. Nakagata, Administration of capsaicin and isoflavone promotes hair growth by increasing insulin-like growth factor-I production in mice and in humans with alopecia. Growth Horm. IGF Res., 2007, 17, 408-415.
15. V.N. Thakare, P.S. Kothavade, V.V. Dhote, A.D. Deshpande, Antifertility activity of ethanolic extract of Allium cepa Linn. in rats, Int. J. PharmTech. Res., 2009, 1, 73-78.
16. A. Kassem, A. Al-Aghbari, M. Al-Habori, M. Al-Mamary, Evaluation of the potential antifertility effect of fenugreek seeds in male and female rabbits, Contraception., 2006, 73, 301-306.
17. M.M. Mohammed, A.E. Mudathir, S.A.I. Shaddad, B. Elsharif, A.A.E.A. Algasem, Antifertility effects of Trigonella foenum-graecum (fenugreek) ethanolic extract in male rats & cocks, J. Pharm. Biomed. Sci., 2013, 32, 1299-1304.
18. A. Sharma, P. Sharma, M. Chaturvedi, S.C. Joshi, Contraceptive efficacy of Citrullus colocynthis methanolic extract in male rats, Am. J. Phytomed. Clin. Therapeut., 2014, 2, 229-241.
19. [19] E.M. Paragas, D. Gehle, K. Krohn, S.G. Franzblau, A.P.G. Macabeo, Anti-tubercular flavonol derivatives from Uvaria rufa, Res. J. Pharm. Biol. Chem. Sci., 2014, 5, 856-859.
20. I.O. Onoagbe, A. Esekheigbe, Studies on the anti-diabetic properties of Uvaria chamae in streptozotocin-induced diabetic rabbits, Biokemistri., 1999, 9, 79-84.
21. G.A. Asare, D. Afriyie, R.A. Ngala, H. Abutiate, D. Doku, S.A. Mohmood, H. Rahman, Antiproliferative activity of aqueous leaf extract of Annona muricata L. on the prostate, BPH-1 cells, and some target genes, Integr. Cancer Ther., 2015, 14, 65-74.
22. W. Buncharoen, K. Saenphet, S. Saenphet, C. Thitaram, Uvaria rufa Blume attenuates benign prostatic hyperplasia via inhibiting 5α-reductase and enhancing antioxidant status, J. Ethnopharmacol., 2016, 194, 483-494.
23. S. Tip-pyang, K. Payakarintarungkul, J. Sichaem, P. Phuwapraisirisan, Chemical constituents from the roots of Uvaria rufa, Chem. Nat. Compd., 2011, 47, 475-476.
24. K. Deepralard, K. Kawanishi, M. Moriyasu, T. Pengsuparp, R. Suttisi, Flavonoid glycosides from the leaves of Uvaria rufa with advanced glycation end-products inhibitory activity. Thai J. Pharm. Sci., 2009, 33, 84-90.
25. S.S. Roh, C.D. Kim, M.H. Lee, S.L. Hwang, M.J. Rang, Y.K. Yoon, The hair growth promoting effect of Sophora flavescens extract and its molecular regulation. J. Dermatol. Sci., 2002, 30, 43-49.
26. G.A. Ayoola, H.A.B. Coker, S.A. Adesegun, A.A. Adepoju-Bellol, K. Obaweyal, E.C. Ezennial, T.O. Atangbayilal, Phytochemical screening and antioxidant activities of some selected medicinal plants used for malaria therapy in Southwestern
Nigeria, Trop. J. Pharm. Res., 2008, 7, 1019-1024.
27. H.O. Edeoga, D.E. Okwu, B.O. Mbaebie, Phytochemical constituents of some Nigerian medicinal plants. Afr. J. Biotechnol., 2005, 4, 685-688.
28. X.W. Chen, E.S. Serag, K.B. Sneed, S.F. Zhou, Herbal bioactivation, molecular targets and toxicity relevance, Chem. Biol. Interact., 2001, 192, 161-176.
29. Z.F. Dewan, I.D. Morris, R.G. Lendon, Administration of exogenous testosterone in the adult rat and its effects on reproductive organs, sex hormones and body weight, Bangladesh Med. Res. Counc. Bull., 2000, 26, 48-55.
30. L. Tόthová, P. Celec, D. Ostatníková, M. Okuliarová, M. Zeman, J. Hodosy, Effect of exogenous testosterone on oxidative status of the testes in adult male rats. Andrologia., 2013, 45, 417-423.
31. S. Prasad, N. Kalra, Y. Shukla, Modulatory effects of diallyl ulphide against testosterone-induced oxidative stress in Swiss albino mice. Asian J. Androl., 2006, 8, 719-723.
32. L.A.J. Shittu, M.A. Bankole, J.A. Oguntola, O. Ajala, R.K. Shittu, O.A. Ogundipe, M.N. Bankole, T. Ahmed, O.A. Ashiru, Sesame leaves intake improve and increase epididymal spermatocytes reserve in adults male Sprague Dawley rat, Sci. Res. Essays., 2007, 2, 319-324.
33. H. Black, Preparation of the report for a toxicology/pathology study, in: W.M. Haschek, C.G. Rousseaux, M.A. Wallig, (Eds.), Handbook of Toxicologic Pathology, first volume, Academic Press, San Diego, CA, 2002, pp. 419-433.
34. R. Mosbah, M.I. Yousef, A. Mantovani, Nicotine-induced reproductive toxicity, oxidative damage, histological changes and haematotoxicity in male rats: the protective effects of green tea extract, Exp. Toxicol. Pathol., 2015, 67, 253-259.
35. M.A. Rezvanfar, R.A. Sadikhanlou, A. Ahmadi, H. Shojaei-Sadee, M.A. Rezvanfar, A. Mohammadirad, A. Salehnia, M. Abdollahi, Protective of cyclophosphamide-induced toxicity in reproductive tract histology, sperm characteristics, and DNA
damage by an herbal source; evidence for role of free-radical toxic stress, Hum. Exp. Toxicol., 2008, 27, 901-910.
36. M. Mohamed, S.A. Sulaiman, H. Jaafar, Histological changes in male accessory reproductive organs in rats exposed to cigarette smoke and the protective effect of honey supplementation, Afr. J. Tradit. Complement. Altern. Med., 2012, 9, 329-335.
37. A. Hentrich, M. Wolter, C. Szardening-Kirchner, G.H. Lüers, M. Bergmann, S. Kliesch, L. Konrad, Reduced numbers of Sertoli, germ, and spermatogonial stem cells in impaired spermatogenesis, Mod. Pathol., 2001, 24, 1380-1389.
38. W. Liao, M. Cai, J. Chen, J. Huang, F. Liu, C. Jiang, Y. Gao, Hypobaric hypoxia causes deleterious effects on spermatogenesis in rats, Reproduction., 2010, 139, 1031-1038.
39. S. Chuma, M. Hosokawa, K. Kitamura, S. Kasai, M. Fujioka, M. Hiyoshi, K. Takamune, T. Noce, N. Nakatsuji, Tdrd1/Mtr-1, a tudor-related gene, is essential for male germ-cell differentiation and nuage/germinal granule formation in mice, Proc. Natl. Acad. Sci. U.S.A., 2006, 103, 15894-15899.
40. D.D. Seachrist, E. Johnson, C. Magee, C.M. Clay, J.K. Graham, D.N. Veeramachaneni, R.V. Keri, Overexpression of follistatin in the mouse epididymis disrupts fluid resorption and sperm transit in testicular excurrent ducts, Biol. Reprod., 2012, 87, 41.
41. Y. Ji, J. Sun, L. Lang, Analysis of testicular toxicity of salanine in mice, Adv. Mater. Res., 2010, 282-283, 195-200.
42. H. Kloer, H. Hoogen, E. Nieschlag, Trial of high-dose testosterone undecanoate in treatment of male infertility, Int. J. Androl., 1980, 3, 121-129.
43. A. M. Matsumoto, Is high dosage testosterone an effective male contraceptive agent?, Fertil. Steril., 1988, 50, 324-328.
44. J.D. Vidal, K.M. Whitney, Morphologic manifestations of testicular and epididymal toxicity, Spermatogenesis., 2014, 4, e979099.