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Formvar is a polyvinyl formaldehyde polymer used to support specimen for transmission electron microscopic (TEM) study. It is appropriate as a film supporting because it has a resistant to electron beam. In this study, formvar films were prepared by casting with 0.20 0.25 0.30 percent formvar solutions for 3 5 8 and 10 seconds on glass slides. The thicknesses of film were observed using an atomic force microscope (AFM). The result showed that the film thickness varied upon the formvar concentrations and the casting times. The suitable film thicknesses were obtained from 0.20 percent solution with 10 seconds, 0.25 percent with 5-10 seconds and 0.30 percent with 3-8 seconds casting times. Overall of this study, it was found that the film thicknesses between 32.14±1.89-56.39±5.30 nanometers are suitable to support the specimens for TEM.
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