Techniques for Preparing Spores and Hyphae of Schizophyllum commune for Morphological Observation.
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Abstract
At present, light microscopy (LM) and electron microscopy (EM) has been an indispensable tool to further our understanding of cellular and subcellular processes of microbiology. The aim of this study was to microscopy techniques that have been used to study the detail of investigating morphological of spores and hyphae of mushrooms such as the structure of the wall pattern, shape, and size of spores and hyphae of mushrooms. Observation morphological of Schizophyllum commune with a light microscope and transmission electron microscope. The experiment showed that the thickness of Schizophyllum commune for the cell walls, spores have an average of 258.83± 29.685 nm. Range 186.00-347.00 nm. and hyphae have an average of 228.25 ± 45.589 nm. Range 144.00-396.00 nm. Observation morphological of Schizophyllum commune with a scanning electron microscope showed that the hyphae and connected by hypha-clam connection, sporangiospores are spores that are produced in a sporangium cell division mitosis phase. However, the technique necessary used in combination with TEM with SEM to yield much better accuracy. As a result, the morphological characteristics of Schizophyllum commune can be used for species identification of this genus. which knowledge on the morphological study of mushroom taxonomy and other applications mushroom tissues. In addition to the knowledge from this research, it can be applied for other scientific information such as agriculture, botany, and environmental science and the agro-food and pharmaceutical industry.
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References
[2]. A. Turkoglu et al. Antioxidant and antimicrobial activities of Laetiporus sulphureus (Bull.) Murrill. Food Chemistry. Food Chemistry.2007, 101 , 267–273.
[3]. B.L. Gabriel, Biological Electron Microscopy. Van Nostrand Reinhold Company Inc., New York. 1982.
[4]. E.S. Reynolds. The use of lead citrate at high pH as an electron opaque stain in electron microscopy. Journal of Cell Biology. 1963, 17, 208–212.
[5]. J. Bozzola. Electron Microscopy Principles and Techniques for Biologists, 1992.
[6]. P. Pandee et al. Production and properties of a fibrinolytic enzyme by Schizophyllum commune BL23.Songklanakarin J. Sci. Technol. Jul. - Aug. 2008, 30 (4), 447-453.
[7]. P. Umroong and P. Kotepong . 2019. Ultrastructure studies of fungus Colletotrichum gloeosporioides cause of Anthracnose disease of mango by microscope technique. in Microsc. Microanal Res. Vol.32 (2) , 1-5.
[8]. P.Rangkhawong et al. Mycelial fermentation in submerged culture of Schizophyllum commune and its properties. J Sci Technol MSU. 2014.Vol 33. No 5, September-October .
[9]. Royal Institute 2007. Mus rooms in Thailand, Royal Institute Edition 2nd edition, Pimlak, Bangkok.
[10]. Suwanno, S and S. Mulang. Enhancement of mycelial and polysaccharide production from Schizophyllum commune
by optimization of culture medium. Journal of Science and Technology Mahasarakham University. 2011. 31(4): 335-343.