First Report, Prevalence, and Molecular Identification of Lecithocladium Cristatum in the Black Pomfret Fish, Parastromateus niger (Family: Carangidae)

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Lipika Oopkaew
Supawadee Kaewphetmadan
Warakorn Bunprasert
Thapana Chontananarth

Abstract

The black pomfret fish, Parastromateus niger, has been reported to have a natural range in the oceanic bay of Thailand, one of the biggest commercial fisheries in the Pacific region. It is an economically important marine fish because it is a favored source of high nutritional value for human consumption. A Lecithocladium infection is considered a problem for the health of fish, human consumption and commercial fisheries. Fifty specimens of P. niger were collected from local markets in Bangkok, Thailand and Lecithocladium spp. was recovered from their digestive tracts.The results showed that all parasite specimens were identified as L. cristatum via morphological characteristics. For molecular identification, the nucleotide sequence data of 18S rRNA were used for molecular analysis. Lecithocladium cristatum in this study showed 97% sequence identity with L. cristatum in the GenBank database. Phylogenetic analysis revealed that this trematode species is deeply embedded within the genus Lecithocladium. The prevalence and mean intensity of L. cristatum infections were 72.00% and 11.07, respectively. Hence, this work revealed the epidemiological situation of L. cristatum in P. niger by using the morphologica- and molecular-based methods. Moreover, this is a first report of L. cristatum in the black pomfret from Thailand. In addition, these data are important for the prevention of future Lecithocladium outbreaks.

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Oopkaew, L. .; Kaewphetmadan, S. .; Bunprasert, W. .; Chontananarth, T. First Report, Prevalence, and Molecular Identification of Lecithocladium Cristatum in the Black Pomfret Fish, Parastromateus Niger (Family: Carangidae). Microsc. Microanal. Res. 2020, 33, 24-27.
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References

Bloch M.E., Naturgeschichte der auslandischen Fische, Vol. 9, Morino, Berlin, 192: 1795, pp. 33.

Cribb T.H., Bray R.A., Littlewood D.T.J., Pichelin S.P. and Herniou E.A., The Digenea. In: Littlewood D.T.J. and Bray R.A. (Eds.), Interrelationships of the Platyhelminthes, Taylor and Francis, London, 2001, pp. 168–185.

Al-Zubaidy A., First record of Lecithochirium sp. (Digenea: Hemiuridae) in the marine fish Carangoides bajad from the Red Sea, Coast of Yemen, Journal of King Abdulaziz University, Marine Science, 21: 2010, 85–94.

Russell B.C., Golani D. and Tikochinski Y., Saurida lessepsianus a new species of lizardfish (Pisces: Synodontidae) from the Red Sea and Mediterranean Sea, with a key to Saurida species in the Red Sea, Zootaxa, 3956 (4): 2015, 559–568.

Yamaguti S., Parasitic worms mainly from Celebes. Part 3. Digenetic trematodes of fishes, Acta Medica Okayama, 8: 1953, 281–283.

Yamaguti S., Synopsis of Digenetic Trematodes of Vertebrates, 1 and 2. Keigaku Publishing Co., Tokyo, 1971.

Reid W.A., Coil W.H. and Kuntz R.E., Hemiruid trematodes of Formosan marine fishes. I. Subfamilies Dinurinae and Stomachicolinae, Journal of parasitology, 52: 1966, 39–45.

Cribb T.H., Chisholm L.A. and Bray R.A., Diversity in the Monogenea and Digenea: does lifestyle matter?, International Journal for Parasitology, 32: 2002, 321–328.

Gibson D.I. and Bray R.A., The Hemiuroidea: terminology, systematics and evolution, Bulletin of the British Museum (Natural History), Zoology, 36: 1979, 35–146.

Luhe M., Uber Hemiuridea, Zoologischer Anzeiger, 24: 1901, 394–488.

Madhavi R. and Lakshmi T.T., Metazoan parasites of the Indian mackerel, Rastrelliger kanagurta (Scombridae) of Visakhapatnam coast, Bay of Bengal, Journal of Parasitic Diseases, 35: 2011, 66–74.

Indaryanto F.R., Abdullah M.F., Wardiatno Y., Tiuria R. and Imai H., A description of Lecithocladium angustiovum (Digenea, Hemiuridae) in short mackerel, Rastrelliger brachysoma (Scombridae) of Indonesia, Tropical Life Sciences Research, 26: 2015, 31–40.

Casanova J.C., Santalla F., Durand P., Vaucher C., Feliu C. and Renaud F., Morphological and genetic differentiation of Rodentolepis straminea (Goeze, 1752) and Rodentolepis microstoma (Dujardin, 1845) (Hymenolepididae), Parasitology Research, 87: 2001, 439–444.

Vilas R., Paniagua E., Outeiral S. and Sanmartin M.L., Electrophoretic and morphological differentiation of three sympatric species of the genus Lecithochirium (Trematoda: Hemiuridae), parasites of marine fishes, Parasitology Research, 88: 2002, 1055–1060.

Criscione C.D. and Blouin M.S., Life cycles shape parasite evolution: comparative population genetics of salmon trematodes, Evolution, 58: 2004, 198–202.

Carreras-Aubets M., Montero F., Kostadinova A., Gibson D. and Carrasson M., Redescriptions of two frequently recorded but poorly known hemiurid digeneans, Lecithochirium musculus (Looss, 1907) (Lecithochiriinae) and Ectenurus lepidus Looss, 1907 (Dinurinae), based on material from the western Mediterranean, Systematic Parasitology, 82: 2012, 185–199.

Luque J. and Oliva M., Trematodes of marine fishes from the Peruvian Faunistic Province (Peru and Chile) with a description of Lecithochirium callaoensis n. sp. and new records, 28, Revista de Biologia Marina, Valparaiso, 1993, 271–286.

Testini G., Papini R., Lia R.P., Parisi A., Dantas-Torres F. and Traversa D., New insights into the morphology, molecular characterization and identification of Baylisascaris transfuga (Ascaridida, Ascarididae), Veterinary Parasitology, 175: 2011, 97–102.

Rudolphi C.A., Entozoorum synopsis, cui accident mantissa duplex et indices locupletissimi. Sumtibus Augusti Rucker, Berolini, 1819, pp. 811.

Lambert A., Environment and host-parasite relationships in monogenea, Folia Parasitologica, 37: 1990, 219–224.

Xenopoulos M.A., Lodge D.M. and Alcamow J., Scenarios of freshwater fish extinctions from climate change and water with-drawal, Global Change Biology, 11: 2005, 1557–1564.

Pedersen A.B. and Fenton A., Emphasizing the ecology in parasite community ecology, Trends in Ecology and Evolution, 22, 2007, 133–139.

Adams A.M., Parasites on the gills of the Plains Killifish, Fundulus kansae, in the South Platte River, Nebraska, Transactions of the American Microscopical Society, 104: 1985, 278–284.

Ferrari-Hoeinghaus A.P., Takemoto R.M., Oliveira L.C., Makrakis M.C. and Baumgartner G., Host-Parasite relationships of monogeneans in gills of Astyanax altiparanae and Rhamdia quelen of the sao francisco

verdadeiro river, Brazil, Journal of parasitology, 13: 2006, 315–320.

Chandra K.J., Hasan M. and Basak S.S., Prevalence of Genarchopsis dasus (Digenea: Hemiuridae) in Channa punctatus of Mymensingh, The Bangladesh Veterinarian, 28(1): 2011, 47–54.

Barber A., Hoare D. and Krause J., Effects of parasites on fish behavior: A review and evolutionary, Reviews in Fish Biology and Fisheries, 10(2): 2000, 131–165.

Lafferty K.D., Ecosystem consequences of fish parasites, Journal of Fish Biology, 73(9): 2008, 2083–2093.

Ruiz G.M., Consequences of parasitism to marine invertebrates host evolution, The American Society of Zoologists, 31(6): 1991, 831–893.

Kabata Z., Parasites and diseases of cultured in the tropics. Philadelphia, USA. Taylor & Francis Incorporated, Journal of Tropical Ecology, 1(2): 1985, 110.

Chambers C.B., Carlisle M.S., Dove A.D.M. and Cribb T.H., A description of Lecithocladium invisorn sp. (Digenea: Hemiuridae) and the pathology associated with two species of Hemiuridae in Acanthurid fish, Parasitology Research, 87(8): 2001, 666–673.

Cribb T.H. and Bray R.A., Gut wash, body soak, blender and heat-fixation: approaches to the effective collection, fixation and preservation of trematodes of fishes, Systematic Parasitology, 76: 2010, 1–7.

Weesner F.M., General Zoological Techniques, The William and Wilkins Company, Baltimore, MD, 1965.

Prapaisiri S., Parasites found in black pomfret Parastromateus niger (Bloch). Dissertation, Kasetsart University, 2005.

Abdel-Gaber, Dalia F., Farid A., Kareem M. and Sherein M., Morphological identification and molecular characterization of 18S rDNA of two hemiurid trematodes (Lecithocladium cristatum Rudolphi, 1819 and Lecithocladium parviovum Yamaguti, 1953) infecting the greater lizardfish Saurida tumbil (Pisces: Synodontidae) inhabiting the Red Sea, Gene, 683: 2019, 243–252.

Blair D. and Barker S.C., Affinities of the Gyliauchenidae: utility of the 18S rRNA gene for phylogenetic inference in the Digenea (Platyhelminthes), International Journal of parasitology, 23: 1993, 527–532.

Guagliardo E.S., Schwerdt C.B., Martorelli S.R., Galeano N.A. and Tanzola R.D., Digenean trematodes of Seriolella porosa (Pisces, Centrolophidae) in San Matias Gulf,nArgentina, Acta Parasitologica, 55: 2010, 29–38.

Bullard S.A., Littorellicola billhawkinsi n. gen., n. sp. (Digenea: Aporocotylidae) from the myocardial lacunae of Florida pompano, Trachinotus carolinus (Carangidae) in the Gulf of Mexico; with a comment on the interrelationships and functional morphology of intertrabecular aporocotylids, Parasitology International, 59: 2010, 587–598.

Blair D., Bray R.A. and Barker S.C., Molecules and morphology in phylogenetic studies of the Hemiuroidea (Digenea: Trematoda: Platyhelminthes), Molecular Phylogenetics and Evolution, 9: 1998, 15-25.