Effect of UV-C Radiation on Seed Germination and Some Ultrastructure in Water Convolvulus (Ipomoea aquatica Forssk. ‘Reptan’)
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Abstract
The objective of this study was to investigate the effects of UV-C radiation on seed germination percentage, germination rate, root length, root thickness and some ultrastructure of water convolvulus (Ipomoea aquatica Forssk. ‘Reptan’). The water convolvulus seeds were exposed to UV-C (254 nm) for period of 2 (1.4 J.cm-2), 4 (2.9 J.cm-2), 8 (5.8 J.cm-2) and 12 (8.7 J.cm-2) hours. The number of germinated seeds was observed every 12 hours for 168 hours. After that, the length and the thickness of the root were measured and studied by semi-thin and ultrathin technique under a light microscope (LM) and a transmission electron microscope (TEM) respectively. The result showed that the UV-C treatment increased germination rate, root length and root thickness. The treatment of 2 hours irradiation had the highest seeds germination percentage (98.89 ± 1.92 %), germination rate (2.49 ± 0.40 seed/hr) and root length (33.60 ± 1.68 mm) when compared with non-irradiated and other UV-C treatments. In addition, the ultrastructure in the root of the 2 hours UV-C treatments under LM and TEM showed an increase in vacuoles in zone of cell division compared to those of the control roots.
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