Comparative of the Morphology and Ultrastructure of Kaffir lime (Citrus hystrix DC.) Leaves Attacked by Citrus Canker Symptoms with Microscopic Techniques

Abstract

The application of microscopic techniques to examine the infestation of pathogens in plants in order to gain further insights into the microbiological processes at the cellular and subcellular level in plants showing disease symptoms are currently commonly used to characterize their morphology and ultrastructure. Therefore, this research aimed to morphological and ultrastructure studies of kaffir lime leaves in citrus diseases. Citrus canker symptoms in Citrus hystrix DC. with transmission electron microscopy and microscopy to visualize how Citrus canker activity in kaffir lime leaves destroyed the ultrastructures. For the study of morphology and cell structure biology the samples must be prepared and infiltrated with resin, embedded, cut and stained. The results showed that morphology and cellular structure of Citrus canker symptoms in kaffir lime leaves were studied with light microscopy and transmission electron microscope. The cuticle of the leaf surface was damaged and the infection found in the mesophyll layer.