Effect of White Nelumbo nucifera on Rat Sperm Motility and Sperm Viability
Main Article Content
Abstract
Nelumbo nucifera Gaertn., also known as Indian lotus, is a large aquatic perennial plant with a long history of use in traditional medicine. According to reports, white N. nucifera petal extract has higher phytochemical compounds with the potential to improve sperm viability and to reduce oxidative stress compared with the red petal extract. Studies of the white N. nucifera petals on the male reproductive system are still not fully explored. This study aimed to determine how white N. nucifera petal tea affects the motility and viability of sperm in adult male rats. Four groups of eight male rats were created (n=8). The experimental groups 2-4 received various concentrations of white N. nucifera tea, while the control group (1) received distilled water. To determine sperm motility and viability, sperm suspension taken from the right caudal epididymis was examined under a light microscope. The results showed the number of motile sperm was significantly increased and the number of non-motile sperm was significantly lower in male rats given white N. nucifera tea at a dose of 0.55 mg/kg BW, when compared to the control group. However, the number of viable sperm did not differ significantly among groups. The indication of enhanced sperm motility can support the usage of white N. nucifera petal tea for improving sperm quality.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
D. Tungmunnithum, D. Pinthong, C. Hano, Flavonoids from Nelumbo nucifera Gaertn., a medicinal plant: Uses in traditional medicine, phytochemistry and pharmacological activities. Medicines, Vol. 5(4), 127 (2018).
W. Wutythamawech, Encyclopedia of Thai herbs and primary pharmaceutical. Odeon Store, Bangkok, 39 (1993).
Ministry of Public Health, Thai herbal pharmacopoeia. 2019, Ministry of Public Health: Nontaburi
S.D. Ayu, B.G.M. Ayu, Padma (Nelumbium Speciosum Willd)-A review. World J. Pharm. Res, Vol. 8, 483-493 (2018).
J. Laoung-On, C. Jaikang, K. Saenphet, P. Sudwan, Phytochemical screening, antioxidant and sperm viability of Nelumbo nucifera petal extracts. Plants, Vol. 10(7), 1375 (2021).
G. Virk, C. Ong, S.S. Du Plessis, Effect of oxidative stress on male reproduction. World J. Mens Health., Vol. 32(1), 1-17 (2014).
M. Darbandi, S.Darbandi, A. Agarwal, P. Sengupta, D. Durairajanayagam, R. Henkel, M.R. Sadeghi, Reactive oxygen species and male reproductive hormones. Reprod. Biol. Endocrin., Vol. 16(1), 1-14 (2018).
K. Makker, A. Agarwal, R. Sharma, Oxidative stress & male infertility. Indian J. Med. Res., Vol. 129(4), 357-367 (2009).
R. Singh, S. Singh, G. Jeyabalan, A. Ali, An overview on traditional medicinal plants as aphrodisiac agent. J. pharmacogn. phytochem., Vol. 1(4), 43-56 (2012).
S. Sakr, Y. Okdah, E. El-Adly, Effect of ginger (Zingiber officinale) on mancozeb fungicide induced testicular damage in albino rats. Aust. J. Basic Appl. Sci., Vol. 3(2), 1328-1333 (2009).
J. Laoung-On, C. Jaikang, K. Saenphet, P. Sudwan, Effect of Nelumbo nucifera petals extract on antioxidant activity and sperm quality in Charolais cattle sperm induced by mancozeb. Plants, Vol. 11(5), 637 (2022).
K. Intui, P. Nuchniyom, J. Laoung-on, C. Jaikang, R. Quiggins, P. Sudwan, Neuroprotective Effect of White Nelumbo nucifera Gaertn. Petal Tea in Rats Poisoned with Mancozeb. Foods, Vol. 12 (11), 2175 (2023).
J. Laoung-on, K. Saenphet, C. Jaikang, P. Sudwan, Effect of Moringa oleifera Lam. Leaf Tea on Sexual Behavior and Reproductive Function in Male Rats. Plants, Vol. 10(10), 2019 (2021).
P. Nuchniyom, K. Intui, J. Laoung-on, C. Jaikang, R. Quiggins, K. Photichai, P. Sudwan, Effects of Nelumbo nucifera Gaertn. Petal Tea Extract on Hepatotoxicity and Oxidative Stress Induced by Mancozeb in Rat Model. Toxics, Vol. 11(6), 480 (2023).
S. Yotarlai, V. Chaisuksunt, K. Saenphet, P. Sudwan, Effects of Boesenbergia rotunda juice on sperm qualities in male rats. J. Med. Plant Res., Vol. 5(16), 3861-3867 (2011).
G. Nenkova, R. Stefanov, M. Chervenkov, V. Alexandrova, Preventive effect of Desferal on sperm motility and morphology. Cell Biochem. Funct., Vol. 34(6), 423-428 (2016).
W. Cao, G.L. Gerton, S.B. Moss, Proteomic profiling of accessory structures from the mouse sperm flagellum. Mol. Cell. Proteom., Vol. 5, 801–810 (2006).
L. Zhengwen, Z. Ming, R.M.M.H. Guido, V. Lily, M. Mohamed, Flavonoids seen through the energy perspective. Int. J. Mol. Sci., Vol. 23, 187 (2021).
L.J. Martin, M. Touaibia, Improvement of testicular steroidogenesis using flavonoids and isoflavonoids for prevention of late-onset male hypogonadism. Antioxidants, Vol. 9, 237 (2020).