Green Synthesis of Copper Nanoparticles Using Anredera cordifolia Stem Extract and Evaluation of Their Antidiabetic Activity
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Abstract
Copper nanoparticles (CuNPs) were successfully synthesized through a green synthesis approach using extract of Anredera cordifolia as a natural reducing and stabilizing agent. The formation of CuNPs was confirmed using several characterization techniques including UV–Visible spectroscopy, Fourier Transform Infrared (FTIR), X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), and Particle Size Analyzer (PSA). UV–Vis analysis showed an absorption peak at approximately 325 nm, indicating the formation of copper nanoparticles. FTIR spectra revealed the presence of functional groups associated with plant metabolites that contribute to nanoparticle reduction and stabilization. PSA analysis showed particle size distribution in the range of 10–150 nm with a dominant peak around 30 nm and a median particle size (D50) of approximately 36 nm. The α-amylase inhibition assay demonstrated that the combination of A. cordifolia extract and CuNPs exhibited stronger inhibitory activity compared to the extract or CuNPs alone, suggesting potential application as a natural antidiabetic agent.
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