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Actinomycetes are a group of bacteria that are widely distributed in soil, litter, water, and other natural sources. These Gram positive bacteria can produce hundreds of bioactive compounds, especially antibiotics. This research isolated culturable actinomycetes from mangrove sediments in the Semarang and Karimunjawa Island areas. The isolates that produce potential antibacterial compounds were identified by qualitative screening using the Biosynthetic Gene Cluster (NRPS/PKS) prediction approach. This research was conducted from June to November 2020. A total of 19 actinomycetes from Semarang and 17 actinomycetes from Karimunjawa were found to have at least one type of Biosynthetic Gene Cluster (NRPS, Type I or Type II PKS), but only three isolates had antibacterial activity against S. aureus, E. coli, and L. monocytogenes. Molecular identification found that the bacteria were similar to Brachybacterium paraconglomeratum (99.92%), Streptomyces pluripotens (100%), and Micromonospora chersina (99.08%). Results of the study concluded that the three bacterial isolates that had bacterial activity have similar genes with known antibiotic-producing genes and can potentially provide new antibiotic candidates.
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Alvarino R, Alonso E, Lacret R, Oves-Costales D, Genilloud O, Reyes F, et al. Caniferolide A, a macrolide from Streptomyces caniferus, attenuates neuroinflammation, oxidative stress, amyloid-beta, and tau pathology in vitro. Molecular Pharmaceutics 2019;16:1456-66.
Ariyanto D, Bengen DG, Prartono T, Wardiatno Y. Short communication: The relationship between content of particular metabolites of fallen mangrove leaves and the rate at which the leaves decompose over time. Biodiversitas 2018;19(3):780-5.
Ariyanto D. Food preference on Telescopium telescopium (Mollusca: Gastropoda) based on food sources in mangrove. Plant Archives 2019;19(1):913-6.
Ariyanto D, Bengen DG, Prartono T, Wardiatno Y. The physicochemical factors and litter dynamics (Rhizophora mucronata lam. and Rhizophora stylosa griff) of replanted Mangroves, Rembang, Central Java, Indonesia. Environment and Natural Resources Journal 2019;17(4):11-29.
Ariyanto D, Bengen DG, Prartono T, Wardiatno Y. Distribution and abundance of Cerithideopsilla djadjariensis (Martin 1899) (potamididae) on avicennia marina in Rembang, Central Java, Indonesia. Egyptian Journal of Aquatic Biology and Fisheries 2020;24(3):323-32.
Carbonell P, Currin A, Jervis AJ, Rattray NJW, Swainston N, Yan C, et al. Bioinformatics for the synthetic biology of natural products: Integrating across the Design-Build-Test cycle. Natural Product Reports 2016;33(8):925-32.
Chevrette MG, Carlson CM, Ortega HE, Thomas C, Ananiev GE, Barns KJ, et al. The antimicrobial potential of Streptomyces from insect microbiomes. Nature Communications 2019; 10(516):1-11.
Corcilius L, Liu DY, Ochoa JL, Linington RG, Payne RJ. Synthesis and evaluation of analogues of the glycinocin family of calcium-dependent antibiotics. Organic and Biomolecular Chemistry 2018;16(29):5310-20.
Davies-Bolorunduro OF, Adeleye IA, Akinleye MO, Wang PG. Anticancer potential of metabolic compounds from marine actinomycetes isolated from Lagos Lagoon sediment. Journal of Pharmaceutical Analysis 2019;9:201-8.
Dhanasekaran D, Thajuddin N, Panneerselvam A. Distribution and ecobiology of antagonistic Streptomyces from agriculture and coastal soil in Tamil Nadu, India. Journal of Culture Collections 2009;6:10-20.
El Samak M, Solyman SM, Hanora A. Antimicrobial activity of bacteria isolated from Red Sea marine invertebrates. Biotechnology Reports 2018;19:e00275.
El Obeid AS, Kamal-Eldin A, Abdelhalim MAK, Haseeb AM. Pharmacological properties of melanin and its function in health. Basic and Clinical Pharmacology and Toxicology 2017;120(6):515-22.
Hamamoto H, Urai M, Ishii K, Yasukawa J, Paudel A, Murai M, et al. Lysocine is a new antibiotic that targets menaquinone in the bacterial membrane. Nature Chemical Biology 2015; 11:127-33.
Hasegawa M, Miura T, Kuzuya K, Inoue A, Ki SW, Horinouchi S, et al. Identification of SAP155 as the target of GEX1A (Herboxidiene), an antitumor natural product. ACS Chemical Biology 2011;6:229-33.
Hayakawa M, Nonomura H. Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. Journal of Fermentation Technology 1987;65(5):501-9.
Hindra, Yang D, Teng Q, Dong L, Huang T, Ge H, et al. Genome mining of Streptomyces mobaraensis dsm40847 as a bleomycin producer providing a biotechnology platform to engineer designer bleomycin analogues. Organic Letters 2017;19(6):1386-9.
Hirsch AM, Valdés M. Micromonospora: An important microbe for biomedicine and potentially for biocontrol and biofuels. Soil Biology and Biochemistry 2010;42:536-42.
Hoffman R, Benz E, Silberstein L, Heslop H, Weitz J, Anastasi J. Hematology: Diagnosis and Treatment. 6th ed. Churchill Livingstone; 2013. p. 311.
Inoue K, Hattori Y, Hino T, Oka H. An approach to on-line electrospray mass spectrometric detection of polypeptide antibiotics of enramycin for high-speed counter-current chromatographic separation. Journal of Pharmaceutical and Biomedical Analysis 2010;51:1154-60.
Kalan L, Gessner A, Thaker MN, Waglechner N, Zhu X, Szawiola A, et al. A cryptic polyene biosynthetic gene cluster in Streptomyces calvus is expressed upon complementation with a functional bldA gene. Chemistry and Biology 2013; 20:1214-24.
Kalkreuter E, Pan G, Cepeda AJ, Shen B. Targeting bacterial genomes for natural product discovery. Trends in Pharmacological Sciences 2019;41(1):13-26.
Karimunjawa National Park. Final Report Karimunjawa: A Community-Based Islandscape Initiative. Kota Kita, Pulau kita, Indonesia: 2019. p. 1-71.
Katz L, Baltz RH. Natural product discovery: Past, present, and future. Journal of Industrial Microbiology and Biotechnology 2016;43(2-3):155-76.
Lee J, Park J, Kim S, Park I, Seo Y. Differential regulation of toxoflavin production and its role in the enhanced virulence of Burkholderia gladioli. Molecular Plant Pathology 2016; 17(1):165-76.
Lee L, Zainal N, Azman A, Eng S, Mutalib NA, Yin W, et al. Streptomyces pluripotens sp . nov ., a bacteriocin-producing streptomycete that inhibits meticillin-resistant Staphylococcus aureus. International Journal of Systematic and Evolutionary Microbiology 2014;64:3297-306.
Maoka T. Carotenoids as natural functional pigments. Journal of Natural Medicines 2019;74:1-16.
Maresca JA, Romberger SP, Bryant DA. Isorenieratene biosynthesis in green sulfur bacteria requires the cooperative actions of two carotenoid cyclases. Journal of Bacteriology 2008;190(19):6384-91.
Messaoudi O, Wink J, Bendahou M. Diversity of actinobacteria isolated from date palms rhizosphere and saline environments: Isolation, identification, and biological activity evaluation. Microorganisms 2020;8(1853):1-19.
Miyanaga A, Janso E, Mcdonald L, He M, Liu H, Barbieri L, et al. Discovery and assembly-line biosynthesis of the lymphostin Salinispora bacteria. Journal of the American Chemical Society 2011;133:13311-3.
Neff EP. Stop and smell the geosmin. Lab Animal Nature 2018;47:267-72.
Nicolaou KC, Tang Y, Wang J. Total synthesis of Sporolide B. Angewandte Chemie International Edition 2009;48(19): 3449-53.
Ningsih SS, Ariyanto D, Puspitasari D, Jayanegara A, Hamim Hamim H, Gunawan H. The amino acid contents in mangrove Rhizophora mucronata leaves in Asahan, North Sumatra, Indonesia. E3S Web of Conferences 2020;151(01047):1-3
Qin Z, Munnoch JT, Devine R, Holmes NA, Seipke RF, Wilkinson KA , et al. Formicamycins, antibacterial polyketides produced by Streptomyces formicae isolated from African Tetraponera plant-ants. Chemical Science 2017;8(4):3218-27.
Radjasa OK, Martens T, Grossart HP, Sabdono A, Simon M, Bachtiar T. Antibacterial property of a coral-associated bacterium pseudoalteromonas luteoviolacea against shrimp pathogenic Vibrio harveyi (In Vitro Study). HAYATI Journal of Biosciences 2005;12(2):77-81.
Rashad FM, Fathy HM, El-Zayat AS, Elghonaimy AM. Isolation and characterization of multifunctional Streptomyces species with antimicrobial, nematicidal, and phytohormone activities from marine environments in Egypt. Microbiological Research 2015;175:34-7.
Romano S, Jackson SA, Patry S, Dobson ADW. Extending the one strain many compounds (OSMAC) principle to marine microorganisms. Marine Drugs 2018;16(7):1-29.
Seipke RF, Hutchings MI. The regulation and biosynthesis of antimycins. Beilstein Journal of Organic Chemistry 2013;9:2556-63.
Sekurova ON, Schneider O, Zotchev SB. Novel bioactive natural products from bacteria via bioprospecting, genome mining and metabolic engineering. Microbial Biotechnology 2019; 12(5):828-44.
Sharma P, Thakur D. Antimicrobial biosynthetic potential and diversity of culturable soil actinobacteria from forest ecosystems of Northeast India. Scientific Reports 2020; 10(1):1-18.
Siregar V, Koropitan AF. Land use change and its impact to marine primary production in Semarang Waters. Procedia Environmental Sciences 2016;33:520-31.
Takeuchi M, Fang CX, Yokota A. Taxonomic Study of the Genus Brachybacterium : Proposal of Brachybacterium conglomeratum sp. nov., nom. rev., Brachybacteriurn paraconglorneratum sp. nov., and Brachybacterium rhamnosum sp. nov. International Journal of Systematic Bacteriology 1995;45(1):160-8.
Thatoi H, Behera BC, Mishra RR, Dutta SK. Biodiversity and biotechnological potential of microorganisms from mangrove ecosystems: A review. Annals of Microbiology 2012;63(1): 1-19.
Tuttle T, Kraka E, Cremer D. Docking , triggering , and biological activity of dynemicin a in DNA: A computational study. Journal of American Chemical Society 2005;127:9469-84.
World Health Organization (WHO). Critically Important Antimicrobials for Human Medicine. Switzerland: WHO publications; 2018. p. 1-45.
Widdick DA, Dodd HM, Barraille P, White J, Stein TH, Chater KF, et al. Cloning and engineering of the cinnamycin biosynthetic gene cluster from Streptomyces cinnamoneus cinnamoneus DSM 40005. Procedings of the National Academy of Sciences of the United States of America 2003;100(7):4316-21.
Yu X, Price NPJ, Evans BS, Metcalfa WW. Purification and characterization of phosphonoglycans from. Journal of Bacteriology 2014;196(9):1768-79.
Zaccai G, Bagyan I, Combet J, Cuello GJ, Demé B, Fichou Y, et al. Neutrons describe ectoine effects on water H-bonding and hydration around a soluble protein and a cell membrane. Scientific Reports 2016;6:1-12.