Biosynthesis of Silver Nanoparticles Using Orange Peel Extract for Application in Catalytic Degradation of Methylene Blue Dye 10.32526/ennrj/19/202100088

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Cathleen Simatupang
Vinod K Jindal
Ranjna Jindal


Interest in the biosynthesis of silver nanoparticles (AgNPs) has been steadily increasing primarily due to their numerous applications in various fields, low-cost, use of non-toxic environmentally-friendly materials and easy implementation. This study focused on the biosynthesis of AgNPs using orange peel extract (OPE), optimization of process conditions, and application in catalytic degradation of methylene blue (MB) dye used in the textile industry. A central composite design in response surface methodology resulted in optimum conditions of 0.0075 g dry peel/mL for OPE concentration, pH of 11 and 1.5 mM silver nitrate concentration. The optimum conditions for the response variables corresponded to the peak absorbance of 0.79 and SPR wavelength of 403.8 nm in UV-vis spectra, and minimum particle size of 12.9 nm. In addition, peak absorbance and SPR wavelength appeared to be related to the size of the AgNPs. A full-factorial design for the catalytic degradation of MB dye by the biosynthesized AgNPs for 1 h indicated the maximum influence of AgNPs compared to the concentrations of MB dye and NaBH4 in decreasing order. The MB dye was reduced rapidly with NaBH4 in the presence of AgNPs due to their catalytic action. The findings of the study show the potential of OPE for the biosynthesis of AgNPs with excellent catalytic activity for the treatment of MB dye in industrial effluent.

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Simatupang, C. ., Jindal, V. K. ., & Jindal, R. . (2021). Biosynthesis of Silver Nanoparticles Using Orange Peel Extract for Application in Catalytic Degradation of Methylene Blue Dye: 10.32526/ennrj/19/202100088. Environment and Natural Resources Journal, 19(6), 468–480. Retrieved from
Original Research Articles


Ahmad S, Munir S, Zeb N, Ullah A, Khan B, Ali J, et al. Green nanotechnology: A review on green synthesis of silver nanoparticles - an ecofriendly approach. International Journal of Nanomedicine 2019;14:5087-107.

Andreescu D, Eastman C, Balantrapu K, Goia DV. A simple route for manufacturing highly dispersed silver nanoparticles. Journal of Materials Research 2007;22(9):2488-96.

Ashkarran AA, Bayat A. Surface plasmon resonance of metal nanostructures as a complementary technique for microscopic size measurement. International Nano Letters 2013;3:50.

Basavegowda N, Lee YR. Synthesis of silver nanoparticles using Satsuma mandarin (Citrus unshiu) peel extract: A novel approach towards waste utilization. Materials Letters 2013; 109:31-3.

Bátori V, Jabbari M, Åkesson D, Lennartsson PR, Taherzadeh MJ, Zamani A. Production of pectin-cellulose biofilms: A new approach for citrus waste recycling. International Journal of Polymer Science 2017;2017:9732329.

Bhakya S, Muthukrishnan S, Sukumaran M, Muthukumar M, Kumar ST, Rao MV. Catalytic degradation of organic dyes using synthesized silver nanoparticles: A green approach. Journal of Bioremediation and Biodegredation 2015;6(5): 1000312.

Bhattarai B, Zaker Y, Bigioni TP. Green synthesis of gold and silver nanoparticles: Challenges and opportunities. Current Opinion in Green and Sustainable Chemistry 2018;12:91-100.

Biswas S, Mulaba-Bafubiandi AF. Optimization of process variables for the biosynthesis of silver nanoparticles by Aspergillus wentii using statistical experimental design. Advances in Natural Sciences: Nanoscience and Nano-technology 2016;7(4):045005.

Bonnia NN, Kamaruddin MS, Nawawi MH, Ratim S, Azlina HN, Ali ES. Green biosynthesis of silver nanoparticles using ‘Polygonum Hydropiper’ and study its catalytic degradation of methylene blue. Procedia Chemistry 2016;19:594-602.

Chinnasamy C, Tamilselvam P, Karthik V, Karthick B. Optimization, and characterization studies on green synthesis of silver nanoparticles using response surface methodology. Advances in Natural and Applied Sciences 2017;11(4): 214-22.

Dalal N, Boruah BS, Neoh A, Biswas R. Correlation of surface plasmon resonance wavelength (SPR) with size and concentration of noble metal nanoparticles. Annals of Reviews and Research 2019;5(2):555658.

De Barros Santos E, Madalossi NV, Sigoli FA, Mazali IO. Silver nanoparticles: Green synthesis, self-assembled nanostructures, and their application as SERS substrates. New Journal of Chemistry 2015;39(4):2839-46.

Dutta T, Chattopadhyay AP, Ghosh NN, Khatua S, Acharya K, Kundu S, et al. Biogenic silver nanoparticle synthesis and stabilization for apoptotic activity; insights from experimental and theoretical studies. Chemical Papers 2020;74:4089-101.

Evanoff Jr DD, Chumanov G. Synthesis and optical properties of silver nanoparticles and arrays. ChemPhysChem 2005;6(7): 1221-31.

Fleger Y, Rosenbluh M. Surface plasmons and surface enhanced Raman spectra of aggregated and alloyed gold-silver nanoparticles. Research Letters in Optics 2009;2009:475941.

Gupta R, Dyer MJ, Weimer WA. Preparation and characterization of surface plasmon resonance tunable gold and silver films. Journal of Applied Physics 2002;92(9):5264-71.

Gupta M, Gularıa P, Sıngh D, Gupta S. Analysis of aroma active constituents, antioxidant, and antimicrobial activity of C. sinensis, Citrus limetta and C. limon fruit peel oil by GC-MS. Bioscıences Biotechnology Research Asia 2014;11(2):895-9.

Haiss W, Thanh NT, Aveyard J, Fernig DG. Determination of size and concentration of gold nanoparticles from UV-Vis spectra. Analytical Chemistry 2007;79(11):4215-21.

Hasan M, Ullah I, Zulfiqar H, Naeem K, Iqbal A, Gul H, et al. Biological entities as chemical reactors for synthesis of nanomaterials: Progress, challenges, and future perspective. Materials Today Chemistry 2018;8:13-28.

Heydari S, Zaryabi MH. Response surface methodology for optimization of green silver nanoparticles synthesized via Phlomis cancellata bunge extract. Analytical and Bioanalytical Chemistry Research 2018;5(2):373-86.

Husain Q. Peroxidase mediated decolorization and remediation of wastewater containing industrial dyes: A review. Reviews in Environmental Science and Bio/Technology 2010;9(2):117-40.

Indana MK, Gangapuram BR, Dadigala R, Bandi R, Guttena V. A novel green synthesis and characterization of silver nanoparticles using gum tragacanth and evaluation of their potential catalytic reduction activities with methylene blue and Congo red dyes. Journal of Analytical Science and Technology 2016;7(1):1-9.

Jana NR, Wang ZL, Pal T. Redox catalytic properties of palladium nanoparticles: Surfactant and electron donor-acceptor effects. Langmuir 2000;16(6):2457-63.

Jamkhande PG, Ghule NW, Bamer AH, Kalaskar MG. Metal nanoparticles synthesis: An overview on methods of preparation, advantages and disadvantages, and applications. Journal of Drug Delivery Science and Technology 2019; 53:101174.

Jyoti K, Singh A. Green synthesis of nanostructured silver particles and their catalytic application in dye degradation. Journal of Genetic Engineering and Biotechnology 2016; 14(2):311-7.

Kahrilas GA, Wally LM, Fredrick SJ, Hiskey M, Prieto AL, Owens JE. Microwave-assisted green synthesis of silver nanoparticles using orange peel extract. ACS Sustainable Chemistry and Engineering 2014;2(3):367-76.

Kaviya S, Santhanalakshmi J, Viswanathan B, Muthumary J, Srinivasan K. Biosynthesis of silver nanoparticles using citrus sinensis peel extract and its antibacterial activity. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2011;79(3):594-8.

Khodadadi B, Bordbar M, Nasrollahzadeh M. Achillea millefolium L. extract mediated green synthesis of waste peach kernel shell supported silver nanoparticles: Application of the nanoparticles for catalytic reduction of a variety of dyes in water. Journal of Colloid and Interface Science 2017;493:85-93.

Menon S, KS SD, Agarwal H, Shanmugam VK. Efficacy of biogenic selenium nanoparticles from an extract of ginger towards evaluation on anti-microbial and antioxidant activities. Colloid and Interface Science Communications 2019;29:1-8.

Nasuha N, Hameed BH, Din AT. Rejected tea as a potential low-cost adsorbent for the removal of methylene blue. Journal of Hazardous Materials 2010;175(1-3):126-32.

Ndolomingo MJ, Bingwa N, Meijboom R. Review of supported metal nanoparticles: Synthesis methodologies, advantages, and application as catalysts. Journal of Materials Science 2020;55(15):6195-241.

Nikaeen G, Yousefinejad S, Rahmdel S, Samari F, Mahdavinia S. Central composite design for optimizing the biosynthesis of silver nanoparticles using plantago major extract and investigating antibacterial, antifungal and antioxidant activity. Scientific Reports 2020;10(1):1-6.

Ozturk B, Parkinson C, Gonzalez-Miquel M. Extraction of polyphenolic antioxidants from orange peel waste using deep eutectic solvents. Separation and Purification Technology 2018;206:1-13.

Patil RS, Kokate MR, Kolekar SS. Bioinspired synthesis of highly stabilized silver nanoparticles using Ocimum tenuiflorum leaf extract and their antibacterial activity. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2012; 91:234-8.

Raj S, Singh H, Trivedi R, Soni V. Biogenic synthesis of AgNPs employing Terminalia arjuna leaf extract and its efficacy towards catalytic degradation of organic dyes. Scientific Reports 2020;10:9616.

Rostami-Vartooni A, Nasrollahzadeh M, Alizadeh M. Green synthesis of seashell supported silver nanoparticles using Bunium persicum seeds extract: Application of the particles for catalytic reduction of organic dyes. Journal of Colloid and Interface Science 2016;470:268-75.

Sabouri MR, Sohrabi MR, Moghaddam AZ. A novel and efficient dyes degradation using bentonite supported zero‐valent iron‐based nanocomposites. Chemistry Select 2020;5(1):369-78.

Saha J, Begum A, Mukherjee A, Kumar S. A novel green synthesis of silver nanoparticles and their catalytic action in reduction of methylene blue dye. Sustainable Environment Research 2017; 27(5):245-50.

Santhanalakshmi J, Venkatesan P. Mono and bimetallic nanoparticles of gold, silver and palladium-catalyzed NADH oxidation-coupled reduction of Eosin-Y. Journal of Nanoparticle Research 2011;13(2):479-90.

Saratale RG, Shin HS, Kumar G, Benelli G, Ghodake GS, Jiang YY, et al. Exploiting fruit byproducts for eco-friendly nanosynthesis: Citrus × clementina peel extract mediated fabrication of silver nanoparticles with high efficacy against microbial pathogens and rat glial tumor C6 cells. Environmental Science and Pollution Research 2018; 25(11):10250-63.

Sethpakdee R. Citrus production in Thailand. Taipei: Food and Fertilizer Technology Center; Extension Bulletin No. 437 [Internet]. 1997 [cited 2021 Jan 20]. Available from:

Shanmuganathan R, Karuppusamy I, Saravanan M, Muthukumar H, Ponnuchamy K, Ramkumar VS, et al. Synthesis of silver nanoparticles and their biomedical applications: A comprehensive review. Current Pharmaceutical Design 2019; 25(24):2650-60.

Suvith VS, Philip D. Catalytic degradation of methylene blue using biosynthesized gold and silver nanoparticles. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2014;118:526-32.

Vanaja M, Paulkumar K, Baburaja M, Rajeshkumar S, Gnanajobitha G, Malarkodi C, et al. Degradation of methylene blue using biologically synthesized silver nanoparticles. Bioinorganic Chemistry and Applications 2014;2014:742346.

Vidhu VK, Philip D. Catalytic degradation of organic dyes using biosynthesized silver nanoparticles. Micron 2014;56:54-62.

Wiley BJ, Im SH, Li ZY, McLellan J, Siekkinen A, Xia Y. Maneuvering the surface plasmon resonance of silver nanostructures through shape-controlled synthesis. Journal of Physical Chemistry B 2006;110(32):15666-75.

Xu L, Wang YY, Huang J, Chen CY, Wang ZX, Xie H. Silver nanoparticles: Synthesis, medical applications, and biosafety. Theranostics 2020;10(20):8996-9031.

Zhang D, Ma XL, Gu Y, Huang H, Zhang GW. Green synthesis of metallic nanoparticles and their potential applications to treat cancer. Frontiers in Chemistry 2020;8:799.

Zollinger H. Color Chemistry: Syntheses, Properties, and Applications of Organic Dyes and Pigments. New York, USA: Wiley-VCH; 1987.