Diversity and Antimicrobial Activity of Plant Growth Promoting Endophytic Actinomycetes Isolated from Thai Orchids 10.32526/ennrj/20/202200039
Article Sidebar
Main Article Content
Abstract
Thirty-two endophytic actinomycetes isolated from 15 Thai orchids were taxonomically studied based on their phenotypic characteristics and 16S rRNA gene sequence analyses (98.97-100.00%). The isolates were identified as Streptomyces including S. parvulus (3 isolates), S. tendae (2 isolates), S. ardesiacus (2 isolates), S. heilongjiangensis (2 isolates), and each of S. daghestanicus, S. antibioticus, S. malaysiensis, S. deserti, S. spiralis, S. thermoviolaceus subsp. apingens, S. globosus, S. collinus, S. olivaceus, and S. zaomyceticus. Micromonospora including M. humi (2 isolates), M. maritima (2 isolates), and each of M. tulbaghiae, M. schwarzwaldensis, M. chersina, M. chalcea M. citrea, and M. aurantiaca; Streptosporangium (2 isolates) including S. sandarakinum and S. pseudovulgare and an isolate of Actinomadura hibisca. Streptomyces (7 isolates), Micromonospora (7 isolates), and Streptosporangium (1 isolate) exhibited antimicrobial activity against Bacillus subtilis ATCC 6633, Kocuria rhizophila ATCC 9341, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, and Candida albicans ATCC 10231. Indole-3-acetic acid (IAA) production of the isolates ranged from 0.04 to 67.30 µg/mL. Isolates DR10-1 and DR9-7 produced high amounts of IAA (58.03 and 67.30 µg/mL) and were selected for optimization. Maximum IAA values obtained were 284.87 and 132.35 µg/mL, using 0.4% L-tryptophan and pH 7 with incubation at 30°C for 13 days. These two isolates enhanced root length, shoot length, number of roots, and fresh weight of rice seedlings (Oryza sativa L. cv. RD49) compared to the control. Results indicated that actinomycetes from Thai orchids were promising sources of antimicrobial compounds and plant hormones for agricultural applications.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Published articles are under the copyright of the Environment and Natural Resources Journal effective when the article is accepted for publication thus granting Environment and Natural Resources Journal all rights for the work so that both parties may be protected from the consequences of unauthorized use. Partially or totally publication of an article elsewhere is possible only after the consent from the editors.
References
Abd-Alla MH, El-Sayed E-SA, Rasmey A-HM. Indole-3-acetic acid (IAA) production by Streptomyces atrovirens isolated from rhizospheric soil in Egypt. Journal of Biology and Earth Sciences 2013;3(2):182-93.
Alibrandi P, Schnell S, Perotto S, Cardinale M. Diversity and structure of the endophytic bacterial communities associated with three terrestrial orchid species as revealed by 16S rRNA gene metabarcoding. Frontiers in Microbiology 2020;11: Article No. 604964.
Arai T. Culture Media for Actinomycetes. Tokyo, Japan: The Society for Actinomycetes Japan; 1975.
Bao X, Shun Q, Chen L. The Medicinal Plants of Dendrobium (Shi-Hu) in China. A Coloured Atlas. Shanghai, Chaina: Press of Fudan University and Press of Shanghai Medical University; 2001.
Berdy J. Bioactive microbial metabolites. The Journal of Antibiotics 2005;58(1):1-26.
Bunyoo C, Duangmal K, Nuntagij A, Thamchaipenet A. Characterisation of endophytic actinomycetes isolated from wattle trees (Acacia auriculiformis A. Cunn. ex Benth.) in Thailand. Genomics and Genetics 2009;2(2):155-63.
Charoenrungruang S, Chanvorachote P, Sritularak B, Pongrakhananon V. Gigantol, a bibenzyl from Dendrobium draconis, inhibits the migratory behavior of non-small cell lung cancer cells. Journal of Natural Products 2014;77(6): 1359-66.
Chutima R, Dell B, Vessabutr S, Bussaban B, Lumyong S. Endophytic fungi from Pecteilis susannae (L.) Rafin (Orchidaceae), a threatened terrestrial orchid in Thailand. Mycorrhiza 2011;21(3):221-9.
Czitrom V. One-factor-at-a-time versus designed experiments. The American Statistician 1999;53(2):126-31.
Etesami H, Alikhani HA, Hosseini HM. Indole-3-acetic acid (IAA) production trait, a useful screening to select endophytic and rhizosphere competent bacteria for rice growth promoting agents. MethodsX 2015;2:72-8.
Faria DC, Dias ACF, Melo IS, de Carvalho Costa FE. Endophytic bacteria isolated from orchid and their potential to promote plant growth. World Journal of Microbiology and Biotechnology 2013;29(2):217-21.
Flores-Gallegos AC, Nava-Reyna E. Plant growth-promoting microbial enzymes. In: Kuddus M, editor. Enzymes in Food Biotechnology. Elsevier; 2019. p. 521-34.
Gangwar M, Rani S, Sharma N. Diversity of endophytic Actinomycetes from wheat and its potential as plant growth promoting and biocontrol agents. Journal of Advanced Laboratory Research in Biology 2012;3(1):13-9.
Gause GF, Preobrazhenskaya TP, Sveshnikova MA, Terekhova LP, Maximova TS. A Guide for the Determination of Actinomycetes. Genera Streptomyces, Streptoverticillium, and Chainia. Moscow, Russia: Nauka; 1983.
Goudjal Y, Toumatia O, Sabaou N, Barakate M, Mathieu F, Zitouni A. Endophytic actinomycetes from spontaneous plants of Algerian Sahara: Indole-3-acetic acid production and tomato plants growth promoting activity. World Journal of Microbiology and Biotechnology 2013;29(10):1821-9.
Herrera H, Fuentes A, Soto J, Valadares R, Arriagada C. Orchid-associated bacteria and their plant growth promotion capabilities. In: Mérillon JM, Kodja H, editors. Orchids Phytochemistry, Biology and Horticulture: Fundamentals and Applications. Switzerland: Springer; 2022. p. 175-200.
Kelly KL. Inter-Society Color Council-National Bureau of Standard Color Name Charts Illustrated with Centroid Colors. Washington DC, USA: Government Printing Office; 1964.
Khamna S, Yokota A, Peberdy JF, Lumyong S. Indole-3-acetic acid production by Streptomyces sp. isolated from some Thai medicinal plant rhizosphere soils. EurAsian Journal of BioSciences 2010;4:23-32.
Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 1980;16(2):111-20.
Klongkumnuankarn P, Busaranon K, Chanvorachote P, Sritularak B, Jongbunprasert V, Likhitwitayawuid K. Cytotoxic and antimigratory activities of phenolic compounds from Dendrobium brymerianum. Evidence-Based Complementary and Alternative Medicine 2015;2015:1-9.
Kongkatitham V, Muangnoi C, Kyokong N, Thaweesest W, Likhitwitayawuid K, Rojsitthisak P, et al. Anti-oxidant and anti-inflammatory effects of new bibenzyl derivatives from Dendrobium parishii in hydrogen peroxide and lipopolysaccharide treated RAW264. 7 cells. Phytochemistry Letters 2018;24:31-8.
Kudo T, Matsushima K, Itoh T, Sasaki J, Suzuki K-I. Description of four new species of the genus Kineosporia: Kineosporia succinea sp. nov., Kineosporia rhizophila sp. nov., Kineosporia mikuniensis sp. nov., and Kineosporia rhamnosa sp. nov., isolated from plant samples, and amended description of the genus Kineosporia. International Journal of Systematic and Evolutionary Microbiology 1998;48(4):1245-55.
Kuncharoen N, Fukasawa W, Mori M, Shiomi K, Tanasupawat S. Diversity and antimicrobial activity of endophytic actinomycetes isolated from plant roots in Thailand. Microbiology 2019;88(4):479-88.
Kuncharoen N, Yuki M, Kudo T, Ohkuma M, Boonchareon A, Mhuantong W, et al. Comparative genomics and proposal of Streptomyces radicis sp. nov., an endophytic actinomycete from roots of plants in Thailand. Microbiological Research 2022;254:Article No. 126889.
Kumar S, Stecher G, Tamura K. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 2016;33(7):1870-4.
Küster E, Williams S. Selection of media for isolation of Streptomycetes. Nature 1964;202(4935):928-9.
Lin L, Xu X. Indole-3-acetic acid production by endophytic Streptomyces sp. En-1 isolated from medicinal plants. Current Microbiology 2013;67(2):209-17.
Maitreesophone P, Khine HEE, Nealiga JQL, Kongkatitham V, Panuthai P, Chaotham C, et al. α-Glucosidase and pancreatic lipase inhibitory effects and anti-adipogenic activity of dendrofalconerol B, a bisbibenzyl from Dendrobium harveyanum. South African Journal of Botany 2022;146:187-95.
Mearns-Spragg A, Bregu M, Boyd K, Burgess J. Cross-species induction and enhancement of antimicrobial activity produced by epibiotic bacteria from marine algae and invertebrates, after exposure to terrestrial bacteria. Letters in Applied Microbiology 1998;27(3):142-6.
Musa Z, Ma J, Egamberdieva D, Mohamad OAA, Abaydulla G, Liu Y, et al. Diversity and antimicrobial potential of cultivable endophytic actinobacteria associated with the medicinal plant Thymus roseus. Frontiers in Microbiology 2020;11:Article No. 191.
Nair DN, Padmavathy S. Impact of endophytic microorganisms on plants, environment and humans. The Scientific World Journal 2014;2014:1-11.
Passari AK, Mishra VK, Saikia R, Gupta VK, Singh BP. Isolation, abundance and phylogenetic affiliation of endophytic actinomycetes associated with medicinal plants and screening for their in vitro antimicrobial biosynthetic potential. Frontiers in Microbiology 2015;7(6):Article No. 273.
Puchooa D. Comparison of different culture media for the in vitro culture of Dendrobium (Orchidaceae). International Journal of Agriculture and Biology 2004;6(5):884-8.
Qin S, Feng W-W, Wang T-T, Ding P, Xing K, Jiang J-H. Plant growth-promoting effect and genomic analysis of the beneficial endophyte Streptomyces sp. KLBMP 5084 isolated from halophyte Limonium sinense. Plant and Soil 2017;416(1):117-32.
Rao HY, Rakshith D, Satish S. Antimicrobial properties of endophytic actinomycetes isolated from Combretum latifolium Blume, a medicinal shrub from Western Ghats of India. Frontiers in Biology 2015;10(6):528-36.
Saitou N, Nei M. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 1987;4(4):406-25.
Sameera B, Prakash HS, Nalini MS. Actinomycetes from the coffee plantation soils of Western Ghats: diversity and enzymatic potentials. International Journal of Current Microbiology and Applied Sciences 2018;7(8):3599-611.
Shah S, Chand K, Rekadwad B, Shouche YS, Sharma J, Pant B. A prospectus of plant growth promoting endophytic bacterium from orchid (Vanda cristata). BMC Biotechnology 2021; 21(1):1-9.
Shakeel Q, Lyu A, Zhang J, Wu M, Chen S, Chen W, et al. Optimization of the cultural medium and conditions for production of antifungal substances by Streptomyces platensis 3-10 and evaluation of its efficacy in suppression of clubroot disease (Plasmodiophora brassicae) of oilseed rape. Biological Control 2016;101:59-68.
Shan W, Zhou Y, Liu H, Yu X. Endophytic actinomycetes from tea plants (Camellia sinensis): Isolation, abundance, antimicrobial, and plant-growth-promoting activities. BioMed Research International 2018;2018:1-12.
Shimizu M. Endophytic actinomycetes: Biocontrol agents and growth promoters. In: Maheshwari DK, editor. Bacteria in Agrobiology: Plant Growth Responses. Berlin Heidelberg, Germany: Springer; 2011. p. 201-20.
Shirling ET, Gottlieb D. Methods for characterization of Streptomyces species1. International Journal of Systematic and Evolutionary Microbiology 1966;16(3):313-40.
Shirokikh I, Zenova G, Merzaeva O, Lapygina E, Batalova G, Lysak L. Actinomycetes in the prokaryotic complex of the rhizosphere of oats in a soddy-podzolic soil. Eurasian Soil Science 2007;40(2):158-62.
Shutsrirung A, Chromkaew Y, Pathom-Aree W, Choonluchanon S, Boonkerd N. Diversity of endophytic actinomycetes in mandarin grown in northern Thailand, their phytohormone production potential and plant growth promoting activity. Soil Science and Plant Nutrition 2013;59(3):322-30.
Sinma K, Nurak T, Khucharoenphaisan K. Potentiality of endophytic actinomycetes isolated from sugar cane. Current Applied Science and Technology 2015;15(2):88-97.
Staneck JL, Roberts GD. Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Applied Microbiology 1974;28(2):226-31.
Stackebrandt E, Rainey FA, Ward-Rainey NL. Proposal for a new hierarchic classification system, Actinobacteria classis nov. International Journal of Systematic and Evolutionary Microbiology 1997;47(2):479-91.
Sukphan P, Sritularak B, Mekboonsonglarp W, Lipipun V, Likhitwitayawuid K. Chemical constituents of Dendrobium venustum and their antimalarial and anti-herpetic properties. Natural Product Communications 2014;9(6):825-7.
Suriyachadkun C, Chunhametha S, Thawai C, Tamura T, Potacharoen W, Kirtikara K, et al. Planotetraspora thailandica sp. nov., isolated from soil in Thailand. International Journal of Systematic and Evolutionary Microbiology 2009;59(5):992-7.
Suwitchayanon P, Chaipon S, Chaichom S, Kunasakdakul K. Potentials of Streptomyces rochei ERY1 as an endophytic actinobacterium inhibiting damping-off pathogenic fungi and growth promoting of cabbage seedling. Chiang Mai Journal of Science 2018;45:692-700.
Taechowisan T, Lumyong S. Activity of endophytic actinomycetes from roots of Zingiber officinale and Alpinia galanga against phytopathogenic fungi. Annals of Microbiology 2003;53(3):291-8.
Tedsree N, Tanasupawat S, Sritularak B, Kuncharoen N, Likhitwitayawuid K. Amycolatopsis dendrobii sp. nov., an endophytic actinomycete isolated from Dendrobium heterocarpum Lindl. International Journal of Systematic and Evolutionary Microbiology 2021;71(7):Article No. 004902.
Tsavkelova EA, Cherdyntseva TA, Botina SG, Netrusov AI. Bacteria associated with orchid roots and microbial production of auxin. Microbiological Research 2007;162(1):69-76.
Williams ST, Cross T. Actinomycetes. In: Booth C, editor. Methods in Microbiology. London, UK: Academic Press; 1971. p. 295-334.
Wu LS, Jia M, Chen L, Zhu B, Dong HX, Si JP, et al. Cytotoxic and antifungal constituents isolated from the metabolites of endophytic fungus DO14 from Dendrobium officinale. Molecules 2016;21(1):Article No. 0014.
Wu W, Chen W, Liu S, Wu J, Zhu Y, Qin L, et al. Beneficial relationships between endophytic bacteria and medicinal plants. Frontiers in Plant Science 2021;12:Article No. 646146.
Xiang XG, Schuiteman A, Li DZ, Huang WC, Chung SW, Li JW, et al. Molecular systematics of Dendrobium (Orchidaceae, Dendrobieae) from mainland Asia based on plastid and nuclear sequences. Molecular Phylogenetics and Evolution 2013;69 (3):950-60.
Xing YM, Chen J, Cui JL, Chen, XM, Guo SX. Antimicrobial activity and biodiversity of endophytic fungi in Dendrobium devonianum and Dendrobium thyrsiflorum from Vietnam. Current Microbiology 2011;62(4):1218-24.
Yoon S-H, Ha S-M, Kwon S, Lim J, Kim Y, Seo H, et al. Introducing EzBioCloud: A taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. International Journal of Systematic and Evolutionary Microbiology 2017;67(5):1613-7.