Exploration of Potential Indigenous Non-phytopathogenic Fungi for Bio-organic Fertilizer Recycling from Organic Waste 10.32526/ennrj/20/202200110

Main Article Content

Abul Hossain Molla
Hasnat Zahan
M. M. H. Oliver
M. Khaled Mosharaf


Using potential microbes in biodegradable solid waste management is an emerging science. Microbes play a significant role in recycling of organic solid wastes. Therefore, the present project was carried out to isolate indigenous potential non-phytopathogenic fungi from local relevant decomposed substrates for the purpose of organic waste recycling as bio-organic fertilizer (BOF). A total of thirteen fungal strains were isolated. Seven of them were identified as Trichoderma spp., and the rest were Penicillium spp. Germination of mung bean (Vigna radiata), mustard (Brassica campestris), and wheat (Triticum aestivum) seeds were assessed by application of 13 fungal isolate suspensions. Significant increase of germination percent was achieved in mung bean (98.35%), mustard (96.65%), and wheat (93.35%) by fungal treatments RW-T02, PL-P01, and CD-T01/MSW-T05, respectively, compared to the controls. But radicle and plumule lengths were not promoted by fungal treatments in the majority of cases. Significantly, the longest radicle and plumule lengths of mung bean and mustard were found in control treatments. Conversely, in wheat the longest radicle and plumule length were achieved in treatments MSW-T05 and RW-T03, respectively. Based on superior performances of percent germination and radicle/plumule length, six fungal isolates were selected for compatibility performance in mixed cultures. In the compatibility tests, two fungal combinations (ABF and BCE) presented superior mutual intermingle appearances. Perhaps these combinations may play significant roles in biodegradation of organic wastes.


Download data is not yet available.

Article Details

How to Cite
Molla, A. H. ., Zahan, H., Oliver, M. M. H. ., & Mosharaf, M. K. (2022). Exploration of Potential Indigenous Non-phytopathogenic Fungi for Bio-organic Fertilizer Recycling from Organic Waste: 10.32526/ennrj/20/202200110. Environment and Natural Resources Journal, 20(6), 598–610. Retrieved from https://ph02.tci-thaijo.org/index.php/ennrj/article/view/247255
Original Research Articles


Adesemoye AO, Torbert HA, Kloepper JW. Enhanced plant nutrient use efficiency with PGPR and AMF in an integrated nutrient management system. Canadian Journal of Microbiology 2008;54(10):876-86.

Ahirwar S, Soni H, Prajapati BP, Kango N. Isolation and screening of thermophilic and thermotolerant fungi for production of hemicellulases from heated environments. Mycology 2017; 8(3):125-34.

Bari MN, Alam MZ, Rahman MM. Soild waste management of Dhaka City using composting process. Proceedings of the International Conference on Biotechnology Engineering; 2007 May 8-10; ICBioE’07, Kuala Lumpur: Malaysia; 2007.

Barua S, Molla AH, Haque MM, Alam MS. Performance of Trichoderma-enriched bio-organic fertilizer in N supplementation and bottle gourd production in field condition. Horticulture International Journal 2018;2(3):106-14.

Bohacz J, Kornillowicz-Kowalska T. Modification of post-industrial lignin by fungal strains of the genus Trichoderma isolated from different composting stages. Journal of Environmental Management 2020;266:Article No. 110573.

Castiglione AM, Mannino G, Contartese V, Bertea CM, Ertani A. Microbial biostimulants as response to modern agriculture needs: Composition, role and application of these innovative products. Plants 2021;10(8):Article No. 1533.

Cerda A, Artola A, Font X, Barrena R, Gea T, Sánchez A. Composting of food wastes: Status and challenges. Bioresource Technology 2018;248:57-67.

Delgado-Sánchez P, Jiménez-Bremont JF, Guerrero-González ML, Flores J. Effect of fungi and light on seed germination of three Opuntia species from semiarid lands of central Mexico. Journal of Plant Research 2013;126:643-9.

Devi A, Anu-Appaiah KA. Mixed malolactic co-culture (Lactobacillus plantarum and Oenococcus oeni) with compatible Saccharomyces influences the polyphenolic, volatile and sensory profile of Shiraz wine. LWT-Food Science and Technology 2021;135:Article No. 1102456.

DeVleeschauwer D, Verdonck O, Assche PV. Phytototoxicity of refuse compost. Biocycle 1981;1-2:44-6.

Duenas R, Tengerdy RP, Gutierrez-Correa M. Cellulase production by mixed fungi in solid-substrate fermentation of bagasse. World Journal of Microbiology and Biotechnology 1995;11:333-7.

Gutierrez-Correa M, Tengerdy RP. Production of cellulase on sugar cane bagasse by fungal mixed culture solid substrate fermentation. Biotechnology Letters 1997;19(7):665-7.

Hassan MM, Daffalla HM, Modwi HI, Osman MG, Ahmed II, Gani MEA, et al. Effects of fungal strains on seeds germination of Millet and Striga Hermonthica. Universal Journal of Agricultural Research 2013;2(2):83-8.

Heerden IV, Cronjé C, Swart SH, Kotzé JM. Microbial, chemical and physical aspects of citrus waste composting. Bioresource Technology 2002;81:71-6.

Islam MM, Kayesh E, Zaman E, Urmi TA, Haque MM. Evaluation of rice (Oryza sativa L.) genotypes for drought tolerance at germination and early seedling stage. The Agriculturists 2018;16(1):44-54.

Jung HY, Kim JK. Eco-friendly waste management of mackerel wastewater and enhancement of its reutilization value. International Biodeterioration and Biodegradation 2016; 111:1-13.

Kagithoju S, Sheikh AH, Godishala V, Nanna RS. Role of fungi and fungal extract on Strychnos potatorum seed germination. Research Journal of Biotechnology 2013;8(11):33-6.

Kauser H, Khwairakpam M. Organic waste management by two-stage composting process to decrease the time required for vermicomposting. Environmental Technology and Innovation 2022;25:Article No. 102193.

Khokhar I, Haider MS, Mukhtar I, Ali A, Mushtaq S, Ashfaq M. Effect of Penicillium species culture filtrate on seedling growth of wheat. International Research Journal of Agricultural Science and Soil Science 2013;3(1):24-9.

Khokhar M, Rawal P, Mathur AC. Effect of fungal metabolites on seed viability and seedling vigour of fenugreek (Trigonella foenum graecum). Jurnal of Mycology and Plant Pathology 2011;41(4):543-5.

Li X, Song M, Yao X, Chai Q, Simpson WR, Li C, et al. The effect of seed-borne fungi and epichloë endophyte on seed germination and biomass of Elymus sibiricus. Frontiers in Microbiology 2017;8:Article No. 2488.

Li Y, Chen L, Tian X, Lin L, Ding R, Yan W, et al. Functional role of mixed-culture microbe in photocatalysis coupled with biodegradation: Total organic carbon removal of ciprofloxacin. Science of the Total Environment 2021;784:Article No. 147049.

Martin JP. Use of acid, rose bengal and streptomycin in the plate for estimating soil fungi. Soil Science 1950;69:215-32.

Mastouri F, Björkman T, Harman GE. Seed treatment with Trichoderma harzianum alleviates biotic, abiotic, and physiological stresses in germinating seeds and seedlings. Phytopathology 2010;100:1213-21.

Meda A, Sangwan P, Bala K. In-vessel composting of HMX and RDX contaminated sludge using microbes isolated from contaminated site. Environmental Pollution 2021;285:Article No. 117394.

Molla AH, Fakhru’l-Razi A, Abd-Aziz S, Hanafi MM, Alam MZ. In-vitro compatibility evaluation of fungal mixed culture for bioconversion of domestic wastewater sludge. World Journal of Microbiology and Biotechnology 2001b;17(9):849-56.

Molla AH, Fakhru’l-Razi A, Abd-Aziz S, Hanafi MM, Roychoudhury PK, Alam MZ. A potential resource for bioconversion of domestic wastewater sludge. Bioresource Technology 2002a;85:263-72.

Molla AH, Fakhru’l-Razi A, Alam MZ. Evaluation of solid-state bioconversion of domestic wastewater sludge as a promising environmental-friendly disposal technique. Water Research 2004;38:4143-52.

Molla AH, Fakhru’l-Razi A, Alam MZ. Solid state bioconversion (SSB): An effective process for safe disposal and recycling of domestic wastewater sludge as compost. The Journal of the Agricultural Engineering Society of Sri Lanka 2006;9(1):57-71.

Molla AH, Fakhru’l-Razi A, Hanafi MM, Abd-Aziz S, Alam MZ. Potential non phytopathogenic filamentous fungi for bioconversion of domestic wastewater sludge. Journal of Environmental Science and Health 2002b;A37(8):1495-507.

Molla AH, Haque MM, Haque MA, Ilias GNM. Trichoderma-enriched biofertilizer enhances production and nutritional quality of tomato (Lycopersicon esculentum Mill.) and minimizes NPK fertilizer use. Agricultural Research 2012;1(3):265-72.

Molla AH, Khan HI. Detoxification of textile effluent by fungal treatment and its performance in agronomic usages. Environmental Science and Pollution Research 2018; 25:10820-8.

Molla AH, Shamsuddin ZH, Halimi MS, Morziah M, Puteh AB. Potential for enhancement of root growth and nodulation of soybean co-inoculated with Azospirillum and Bradyrhizobium in laboratory systems. Soil Biolology and Biochemistry 2001a;33:457-63.

Molla AH. Solid State Bioconversion of Domestic Wastewater Treatment Plant Sludge into Compost by Screened Filamentous Fungi [dissertation]. Faculty of Engineering, Universiti Putra Malaysia, Malaysia; 2002.

Rahman KSM, Banat IM, Thahira J, Thayumanavan T, Lakshmanaperumalsamy P. Bioremediation of gasoline contaminated soil by a bacterial consortium amended with poultry litter, coir pith and rhamnolipid biosurfactant. Bioresource Technology 2002;81:25-32.

Rao VK, Girisham S, Reddy SM. Influence of different species of Penicillium and their culture filtrates on seed germination and seedling growth of sorghum. Journal of Biochemistry and Technology 2014;5(4):832-7.

Rifai MA. A revision of the genus Trichoderma. Mycological Papers No. 116. Kew, Surrey, England: Commonwealth Mycological Institute; 1969.

Srivastava B, Khatri M, Singh G, Arya SK. Microbial keratinases: An overview of biochemical characterization and its eco-friendly approach for industrial applications. Journal of Cleaner Production 2020;252:Article No. 119847.

Tamura T, Hashidoko T, Ogita N, Limin SH, Tahara S. Requirement for particular seed-borne fungi for seed germination and seedling growth of Xyris complanata, a pioneer monocot in topsoil-lost tropical peatland in Central Kalimantan, Indonesia. Ecological Research 2008;23:573-9.

Yamaji K, Hashidoko Y, Fukushi Y, Tahara S. Chemical response of Picea glehnii seed-epiphytic Penicillium species to Pythium vexans under in vitro competitive conditions for mycelial growth. Journal of Chemical Ecology 2005;31:805-17.

Yoneyama K, Takeuchi Y, Ogasawara M, Konnai M, Sugimoto Y, Sassa T. Cotylenins and fusicoccins stimulate seed germination of Striga hermonthica (Del.) Benth and Orobanche minor Smith. Journal of Agricultural and Food Chemistry 1998;46:1583-6.

Young D, Dollhofer V, Callaghan TM, Reitberger S, Lebuhn M, Benz JP. Isolation, identification and characterization of lignocellulolytic aerobic and anaerobic fungi in one- and two-phase biogas plants. Bioresource Technology 2018;268:470-9.

Yue MLi, Shaffer JP, Hall B, Ko H. Soil-borne fungi influence seed germination and mortality, with implications for coexistence of desert winter annual plants. PLoS One 2019;31:1-21.