The Effectiveness of an Anoxic-Oxic-Anoxic-Oxic Sequencing Batch Reactor System (A2/O2-SBR) to Treat Electroplating Wastewater and the Bacterial Community within the System 10.32526/ennrj/23/20240134

Main Article Content

Tanta Suriyawong
Sasidhorn Buddhawong
Thanit Swasdisevi
Suntud Sirianuntapiboon

Abstract

This study presents an examination of the effectiveness of an anoxic-oxic-anoxic-oxic sequencing batch reactor (A2/O2-SBR) for treating electroplating wastewater (EPWW). The A2/O2-SBR was monitored for 60 days and the bacterial composition in the treatment system was determined. The system consisted of four reactors, which had the following anoxic/oxic ratios: reactor-I, 0:9 h; reactor-II, 2:7 h; reactor-III, 4.5:4.5 h; and reactor-IV, 7:2 h. The combined cycle time of the reactors was 12 h, the hydraulic retention time (HRT) was five days, and the total volume of mixed liquor suspended solids (MLSS) was 2,000 mg/L. The results demonstrate the importance of an anoxic period for the treatment of heavy metals. Most of the ammonium nitrogen (NH4+-N), Total Kjeldahl nitrogen (TKN), and total nitrogen (TN) were removed during the oxic period. However, as the anoxic period increased, the amounts of TKN, nitrite nitrogen (NO2--N), nitrate nitrogen (NO3--N), and TN declined. Reactor-IV showed a high removal efficiency for heavy metals (Zn2+, 89.74%; Cd2+, 81.37%), TKN (89.20%), and TN (84.25%), and also effectively treated NH4+-N (78.84%), biochemical oxygen demand (BOD5; 93.5%), and chemical oxygen demand (COD; 84.9%). Reactor-IV showed an appropriate difference in the dissolved oxygen (DO) concentration between the anoxic period and oxic period (2.12-2.00 mg/L). The main bacterial phyla in the treatment system were Proteobacteria, Actinobacteria, and Firmicutes, while Pseudomonas vancouverensis and Cryobacterium arcticum were the most common species. The anoxic period and bacterial community have significantly demonstrated the ability to remove Zn2+ and Cd2+ for effective treatment of EPWW.

Article Details

How to Cite
Suriyawong, T., Buddhawong, S., Swasdisevi, T., & Sirianuntapiboon, S. (2024). The Effectiveness of an Anoxic-Oxic-Anoxic-Oxic Sequencing Batch Reactor System (A2/O2-SBR) to Treat Electroplating Wastewater and the Bacterial Community within the System: 10.32526/ennrj/23/20240134. Environment and Natural Resources Journal, xx. retrieved from https://ph02.tci-thaijo.org/index.php/ennrj/article/view/253960
Section
Original Research Articles

References

Abbas S, Rafatullah M, Ismail N, Lalung J. Isolation, identification, and characterization of cadmium resistant Pseudomonas sp. M3 from industrial wastewater. Journal of Waste Management 2014;2014(1):Article No. 160398.

Ayangbenro A, Babalola O. A new strategy for heavy metal polluted environments: A review of microbial biosorbents. International Journal of Environmental Research and Public Health 2017;14(1): Article No. 94.

Bi Z, Qiao S, Zhou J, Tang X, Cheng Y. Inhibition and recovery of Anammox biomass subjected to short-term exposure of Cd, Ag, Hg, and Pb. Chemical Engineering Journal 2014;244:89-96.

Chen J, Xu Y, Li Y, Liao J, Ling J, Li J, et al. Effective removal of nitrate by denitrification re-enforced with a two-stage anoxic/oxic (A/O) process from a digested piggery wastewater with a low C/N ratio. Journal of Environmental Management 2019;240:19-26.

Coelho F, Oliveira V, Araujo E, Balarini J, Konzen C, Salum A, et al. Treatment of a wastewater from a galvanizing industry containing chromium (VI) and zinc (II) by liquid surfactant membranes technique. Journal of Environmental Science and Health, Part A 2021;56(3):289-302.

Dai M, Zhou G, Ng H, Zhang J, Wang Y, Li N, et al. Diversity evolution of functional bacteria and resistance genes (CzCA) in aerobic activated sludge under Cd(II) stress. Journal of Environmental Management 2019;250:Article No. 109519.

Fathollahi A, Khasteganan N, Coupe S, Newman A. A meta-analysis of metal biosorption by suspended bacteria from three phyla. Chemosphere 2021;268:Article No. 129290.

Feng B, Fang Z, Hou J, Ma X, Huang Y, Huang L. Effects of heavy metal wastewater on the anoxic/aerobic-membrane bioreactor bioprocess and membrane fouling. Bioresource Technology 2013;142:32-8.

Gabarro J, Gonzalez-Carcamo P, Ruscalleda M, Ganigue R, Gich F, Balaguer M, et al. Anoxic phases are main N2O contributor in partial nitritation reactors treating high nitrogen loads with alternate aeration. Bioresource Technology 2014;163:92-9.

Gong L, Jun L, Yang Q, Wang S, Ma B, Peng Y. Biomass characteristics and simultaneous nitrification-denitrification under long sludge retention time in an integrated reactor treating rural domestic sewage. Bioresource Technology 2012;119:277-84.

Guo H, Yao H, Huang Q, Lia T, Show D, Ling M, et al. Anaerobic-anoxic-oxic biological treatment of high-strength, highly recalcitrant polyphenylene sulfide wastewater. Bioresource Technology 2023;371:Article No. 128640.

Gao J, Duan C, Huang X, Yu J, Cao Z, Zhu J. The tolerance of anoxic-oxic (A/O) process for the changing of refractory organics in electroplating wastewater: Performance, optimization and microbial characteristics. Processes 2021; 9(6):Article No. 962

Hu B, Wang Y, Quan J, Huang K, Gua X, Zhu J, et al. Effects of static magnetic field on the performances of anoxic/oxic sequencing batch reactor. Bioresource Technology 2020;309:Article No. 123299.

Hu Z, Zhang J, Xie H, Li S, Wang J, Zhang T. Effect of anoxic/aerobic phase fraction on N2O emission in a sequencing batch reactor under low temperature. Bioresource Technology 2011;102(9):5486-91.

Huang X, Zhu J, Duan W, Gao J, Li W. Biological nitrogen removal and metabolic characteristics in a full-scale two-staged anoxic-oxic (A/O) system to treat optoelectronic wastewater. Bioresource Technology 2020;300:Article No. 122595.

Huang Z, Liu D, Zhao H, Zhang Y, Zhang Y, Zhou W. Performance and microbial community dynamic membrane bioreactor enhanced by Cd(II)-accumulating bacterium in Cd(II)-containing wastewater treatment. Chemical Engineering Journal 2017;317:368-75.

Hussain S, Khan M, Sheikh T, Mumtaz M, Chohan T, Shamim S, et al. Zinc Essentiality, toxicity, and its bacteria bioremediation. Frontiers in Microbiology 2022;13:Article No. 900740.

Igbinosa I, Nwodo U, Sosa A, Tom M, Okoh A. Commensal Pseudomonas Species isolated from wastewater and freshwater Milieus in the Eastern Cape Province, South Africa, as reservoir of antibiotic resistant determinants. International Journal of Environmental Research and Public Health 2012;9(7):2537-49.

James S, Vijayanandan A. Anoxic-aerobic-anoxic sequencing batch reactor for enhanced nitrogen removal. Bioresource Technology 2022;363:Article No. 127892.

Jaroszynski LW, Cicek N, Sparling R, Oleszkiewicz JA. Importance of the operating pH in maintaining the stability of anoxic ammonium oxidation (anammox) activity in moving bed biofilm reactors. Bioresource Technology 2011;102(14):7051-6.

Jena J, Kumarb R, Saifuddina M, Dixitb A, Das T. Anoxic-aerobic SBR system for nitrate, phosphate and COD removal from high-strength wastewater and diversity study of microbial communities. Biochemical Engineering Journal 2016;105:80-9.

Jena J, Narward N, Das T, Dhotre D, Sarkar U, Souche Y. Treatment of industrial effluents and assessment of their impact on the structure and function of microbial diversity in a unique anoxic-aerobic sequencing bath reactor (AnASBR). Journal of Environmental Management 2020;261:Article No. 110241.

Jin Y, Xiong W, Zhou N, Xiao G, Wang S, Su H. Role of initial bacterial community in the aerobic sludge granulation and performance. Journal of Environmental Management 2022;309:Article No. 114706.

Kalita N, Baruah P. Cyanobacteria as a potent platform for heavy metals biosorption: Uptake, responses and removal mechanisms. Journal of Hazardous Materials Advances 2023; 11:Article No. 100349.

Kimura Y, Isaka K, Kazama F. Tolerance level of dissolved oxygen to feed into anaerobic ammonium oxidation (anammox) reactor. Journal of Water and Environment Technology 2011;9(2):171-8.

Li S, Xu Q, Ma B, Guo L, She Z, Zhao Y, et al. Performance evaluation and microbial community of a sequencing batch reactor under divalent cadmium (Cd(II)) stress. Chemical Engineering Journal 2018;336:325-33.

Liu J, Zhang H, Zhang P, Wu Y, Gou X, Song Y, et al. Two-stage anoxic/oxic combined membrane bioreactor system for landfill leachate treatment: Pollutant removal performances and microbial community. Bioresource Technology 2017; 243:738-46.

Liu Y, Ma B, Liu Z. Application of partial nitrogen lab-scale sequencing batch reactor for the treatment of organic wastewater and its N2O production pathways, and the microbial mechanism. Sustainability 2022;14(3):Article No. 1457.

Lu W, Ma B, Wang Q, Wei Y, Su Z. Feasibility of achieving advance nitrogen removal via endogenous denitrification/ annamox. Bioresource Technology 2021;325:Article No. 124666.

Marques A, Duque A, Bessa V, Mesquita R, Rangel A, Castro P. Performance of an aerobic granular sequencing batch reactor fed with wastewater contaminated with Zn2+. Journal of Environmental Management 2013;128:877-82.

Quan Y, Han H, Zheng S. Effect of dissolved oxygen concentration (microaerobic and aerobic) on selective enrichment culture for bioaugmentation of acidic industrial wastewater. Bioresource Technology 2012;120:1-5.

Rice E, Baird R, Eaton A, Clesceri L. Standard Methods for the Examination of Water and Wastewater. Washington DC, USA: American Public Health Association, American Water Works Association, Water Environment Federation; 2012.

Singh A, Srivastava A, Saidulu D, Gupta A. Advancements of sequencing batch reactor for industrial wastewater treatment: Major focus on modifications, critical operational parameters, and future perspectives. Journal of Environment Management 2022;317:Article No. 115305.

Sirianuntapiboon S. Effect of the dilution rate and hydraulic retention time on the efficiency of the sequencing batch reactor (SBR) system with electroplating wastewater. Journal of Environmental Chemical Engineering 2013;1(4):786-94.

Su J, Wang Z, Huang T, Zhang H, Zhang H. Simultaneous removal of nitrate, phosphorus and cadmium using a novel multifunctional biomaterial immobilized aerobic strain Proteobacteria cupriavidus H29. Bioresource Technology 2020;307:Article No. 123196.

Su J, Xue L, Huang T, Wei L, Gao C, Wen Q. Performance and microbial community of simultaneous removal of NO3--N, Cd2+, and Ca2+ in MBBR. Journal of Environmental Management 2019;250:Article No. 109548.

Tang G, Li B, Zhang B, Wang C, Zeng G, Zheng X, et al. Dynamics of dissolved organic matter and dissolved organic nitrogen during anaerobic/anoxic/oxic treatment process. Bioresource Technology 2021;331:Article No. 125026.

Wang W, Cui J, Li J, Du J, Chang Y, Cui J, et al. Removal effects of difference emergent-aquatic-plant groups on Cu, Zn, and Cd compound pollution from simulated swine wastewater. Journal of Environmental Management 2021;296:Article No. 113251.

Wang Y, Ji M, Zhao Y, Zhai H. Recovery of nitrification in cadmium-inhibited activated sludge system by bio-accelerators. Bioresource Technology 2016;200:812-9.

Wu D, Zhang Z, Yu Z, Zhu L. Optimization of F/M ratio for stability of aerobic granular process via quantitative sludge discharge. Journal of Bioresource Technology 2018;252:150-6.

Xie Y, Zhang Q, Wu Q, Zhang J, Dzakpasu M, Wang X. Nitrogen removal efficiency and mechanisms of an improved anaerobic-anoxic-oxic system for decentralized sewage treatment. Bioresource Technology 2024;393:Article No. 129976.

Yan L, Liu S, Liu Q, Zhang M, Liu Y, When Y, et al. Improved performance of simultaneous nitrification and denitrification via nitrite in an oxygen-limited SBR by alternating the DO. Bioresearch Technology 2019;275:153-62.

Yan X, Zhu C, Huang B, Yan Q, Zhang G. Enhanced nitrogen removal from electroplating tail wastewater through two-staged anoxic-oxic (A/O) process. Bioresource Technology 2018;247:157-64.

Yong Z, Bashir M, Ng C, Sethupathi S, Lim J. A sequential treatment of intermediate tropical landfill leachate using a sequencing batch reactor (SBR) and coagulation. Journal of Environmental Management 2018;205:244-52.

Zhang H, Yan D, Zhu Y, Li Y, Zhang G, Jiao Y, et al. Effect of Cd(II) shock loading on performance, microbial enzymatic activity and microbial community in a sequencing batch reactor. Journal of Environmental Management 2023; 342:Article No. 118108.

Zhang L, Liu Y, Wang C, Guo J, Ma D, Liu S, et al. The effects of varying Zn2+ on the activated sludge properties and its distribution patterns. Bioresource Technology Reports 2022;17:Article No. 100990.

Zheng Z, Ali A, Su J, Zhang S, Fan Y, Sun Y. Self-immobilized biochar fungal pellet combined with bacterial strain H29 enhanced the removal performance of cadmium and nitrate. Bioresource Technology 2021;341:Article No. 125803.