Development of Virtual Serum for Antibody Screening on the Laboratory Teaching in the Transfusion Science Subject
Keywords:
Virtual Serum, Antibody screening, Albumin, YellowAbstract
Objective: To prepare a virtual serum that has a yellow and light yellow color similar to the color of serum obtained from human blood for use in antibody screening that gives negative and positive results by the conventional tube test (CTT) and the column agglutination technique (CAT).
Methods: Virtual serum from previous study was prepared by reducing the amount of albumin to 0.1 g/dL without and with added anti-D (IgG). The virtual serum was diluted to dilution ratios of 1:2, 1:4, and 1:8. The naked eyes reviewed the appearance of the virtual serum. Optical density and specific gravity were measured and compared between dilution ratios using the Kruskal-Wallis test statistics. Antibody screening was performed using CTT and CAT.
Results: Virtual serum with different dilution ratios was viewed with the naked eye, which gave different shades of yellow and light yellow. The optical density and specific gravity were statistically significant different (p < 0.050). The results of the antibody screening without and with added anti-D (IgG) at every dilution accurate ratio showed accurate negative results with no false positives and authentic positive results with no false negatives.
Conclusion: Virtual serum prepared by reducing the amount of albumin to 0.1 g/dL with varying ratios of dilution had a distinct yellow appearance. Antibody screening using CTT and CAT that are truly negative and positive results without false positives and false negatives, respectively.
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