Electrospun centella asiatica leaf extract–loaded poly (vinyl alcohol)/ gelatin fiber mats as potential wound dressings
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Abstract
The electrospun poly(vinyl alcohol) (PVA)/gelatin (GEL) fibers containing Centella asiatica leaf extract (CA) with differentratios of PVA/GEL and different contents of CA were fabricated. The ratios of PVA/GEL/CA affected the kinematic viscosities of solution and thereby affected the morphology and size of fibers. Only the electrospun fiber mats at PVA/GEL ratios of 9/1 and 8/2 containing 5% CA (PVA/GEL/CA 9/1/5 and 8/2/5) were selected to investigate their potential for use as wound dressings. The release behaviors of CA from these fiber mats were investigated at 37°C in phosphate buffer (pH 7.4) solution using the total immersion and the diffusion using modified Franz cell methods. For both release methods, the burst release of CA at the initial time followed by a gradual release until reaching a plateau was noticed. The PVA/GEL/CA 8/2/5 provided a greater release of CA than the PVA/GEL/CA 9/1/5 fiber mats. The slower rate and smaller amounts of CA released from the diffusion method than those from the total immersion method were observed. The degree of water swelling of the fiber
mats was evaluated. Lastly, the PVA/GEL/CA 9/1/5 and 8/2/5 fiber mats exhibited antioxidant activity as determined by 1,1–diphenyl–2–picrylhydrazyl (DPPH) assay and antibacterial activity against Staphylococcus aureus and Escherichia coli, which revealed the potential for use as wound dressing materials.
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