Ethanolic Extract of Piper betle L. Leaves for Controlling of Bovine Mastitis

Abstract

Antibiotic use may induce bacterial resistance, especially with dairy mastitis. At present, plant extracts are being researched as an alternative to antibiotic use. In this research, betel leaf extract that inhibited both Gram-positive and Gram-negative pathogenic bacteria was applied to study the efficacy of prevention and treatment of mastitis in dairy cows. Betel leaves were extracted with 95% ethanol. The resulting extracts were examined for antibacterial activity by Agar disc diffusion and Broth micro-dilution methods. The resulting extract was then prepared with betel leaf extract solution, then the solution was tested for use with dairy cows. The results showed that the betel leaf extract had antibacterial activity against Staphylococcus aureus and Escherichia coli with values of minimal inhibitory concentrations at 0.62 mg/ml and 2.5 mg/ml, respectively. For the results of testing in dairy cows, betel leaf extract was able to prevent infection in the cows with statistically significantly compared to Masodine. Betel leaf extract solution is used as a breast infusion solution to treat mastitis in dairy cows. The results showed that the reagent was able to reduce the somatic cells in milk when used continuously over a period of 7 days. This research provides promising results in developing betel leaf extract as a substitute for antibiotics in livestock farms.