Nitrogen removal from swine wastewater using Chlorella vulgaris immobilized-cell
Keywords:
Swine wastewater, Microalgae immobilized-cell, Ammonia removalAbstract
This study aimed to evaluate the efficiency of nitrogen and organic matter removal from swine farm wastewater using immobilized microalgae Chlorella vulgaris (C. vulgaris). The C. vulgaris culture was grown in the laboratory for 11 days using the standard liquid medium Blue-Green Medium (BG-11) under a light intensity of 1,800 Lux. The experiment focused on assessing nitrogen removal efficiency using immobilized C. vulgaris cells entrapped with sodium alginate. The result indicated that the immobilized microalgae effectively removed nitrogen from swine farm wastewater. Initial concentrations of ammonia, nitrite, and nitrate in the 100% swine wastewater (SW) reactor were 440 mgN L-1, 0 mgN L-1, and 2.5 mgN L-1, respectively. In the 50% SW reactor, the initial concentrations of ammonia, nitrite, and nitrate were 228 mgN L-1, 0 mgN L-1, and 1.3 mgN L-1, respectively. After 13 days of operation, both the 100% SW and 50% SW reactors achieved 100% ammonia removal efficiency.
References
APHA. (2012). Standard methods for the examination of water and wastewater (21st edition). American Public Health Association/American Water Works Association/Water Environment Federation.
Cao, S., Teng, F., Lv, J., Zhang, Q., Wang, T., Zhu, C., Li, X., Cai, Z., Xie, L., & Tao, Y. (2022). Performance of an immobilized microalgae-based process for wastewater treatment and biomass production: Nutrients removal, lipid induction, microalgae harvesting and dewatering. Bioresource technology, 356, 127298.
Chen, C. Y., Kuo, E. W., Nagarajan, D., Dong, C. D., Lee, D. J., Varjani, S., Lam, S.S., & Chang, J. S. (2021). Semi-batch cultivation of Chlorella sorokiniana AK-1 with dual carriers for the effective treatment of full strength piggery wastewater treatment. Bioresource technology, 326, 124773.
de-Bashan, L.E., & Bashan, Y. (2010). Immobilized microalgae for removing pollutants: review of practical aspects. Bioresource Technology, 101(6), 1611–1627.
Ding, J.F., Zhao, F.M., Cao, Y.F., Xing, L., Liu, W., Mei, S., & Li S.J. (2014) Cultivation of microalgae in dairy wastewater without sterilization. International Journal of Phytoremediation, 17, 222–227.
Emparan, Q., Harun, R., & Sing Jye, Y. (2021). Efficiency of pollutants removal in treated palm oil mill effluent (TPOME) using different concentrations of sodium alginate-immobilized Nannochloropsis sp. cells. International Journal of Phytoremediation, 23(5), 454–461.
Jaafarzadeh Haghighi Fard., N.A., Jorfi, S., Ahmadi, M., Mirali, S., & Kujlu, R. (2016). Treatment of mature landfill leachate by chemical precipitation and Fenton advanced oxidation process. Environmental Health Engineering and Management Journal, 3(1), 35-40.
Kube, M., Mohseni, A., Fan, L., Roddick, F. (2019). Impact of alginate selection for wastewater treatment by immobilized Chlorella vulgaris. Chemical Engineering Journal, 358, 1601-1609.
Kwon, G., Nam, J.H., Kim, D.M., Song, C., & Jahng, D. (2020). Growth and nutrient removal of Chlorella vulgaris in ammonia-reduced raw and anaerobically-digested piggery Wastewaters. Environmental Engineering Research, 25(2), 135-146.
Li, S., Show, P.L., Ngo, H.H., & Ho, S.H. (2022). Algae-mediated antibiotic wastewater treatment: A critical review. Environmental Science and Ecotechnology, 9, 100145.
Martínez, M.E., Camacho, F., Jiménez, J., & Espinola, J. (1997). Influence of light intensity on the kinetic and yield parameters of Chlorella pyrenoidosa mixotrophic growth. Process Biochemistry, 32(2), 93-98.
Mohsenpour, S. F., Hennige, S., Willoughby, N., Adeloye, A., & Gutierrez, T. (2021). Integrating micro-algae into wastewater treatment: A review. The Science of the total environment, 752, 142168.
Moreno-Garrido I. (2008). Microalgae immobilization: current techniques and uses. Bioresource Technology, 99(10), 3949–3964.
Pang, N., Bergeron, A.D., Gu, X., Fu, X., Dong, T., Yao, Y., & Chen, S. (2020). Recycling of Nutrients from Dairy Wastewater by Extremophilic Microalgae with High Ammonia Tolerance. Environmental Science & Technology,
(23), 15366–15375.
Park, J., Jin, H.F., Lim, B.R., Park, K.Y, & Lee, K. (2010). Ammonia removal from anaerobic digestion effluent of livestock waste using green alga Scenedesmus sp. Bioresource Technology, 101(22), 8649–8657.
Rajivgandhi., G., Ramachandran, G., Chelliah, C.K., Maruthupandy, M., Quero, F., Vijayalakshmi S., AL-Mekhlafi., F.A., Muhammad, A. Wadaan, M.A., Ranjitha, J., & Li, WJ. (2022). Green microalgal strain Chlorella vulgaris isolated from industrial wastewater with remediation capacity. Environmental Technology & Innovation, 28. 102597.
Shen, Y., Gao, J., & Li, L. (2017). Municipal wastewater treatment via co-immobilized microalgal-bacterial symbiosis: microorganism growth and nutrients removal, Bioresource Technology. 243, 905-913.
Sutherland, D.L., & Ralph, P.J. (2019). Microalgal bioremediation of emerging contaminants - Opportunities and challenges. Water research, 164, 114921.
Tam, N.F.Y., & Wang, Y.S. (1996). Effect of ammonia concentrations on growth of Chlorella vulgaris and nitrogen removal from media. Bioresource Technology, 57(1), 45–50.
Weidong, L., Wang, Z., Wang, X., & Yuan, Z. (2015). Cultivation of Chlorella sp. using raw diary Wastewater for nutrient removal and biodiesel production: characteristics comparison of indoor bench-scale and outdoor pilot-scale cultures. Bioresource Technology, 192, 382–388
Wu, J.Y., Lay, C.H., Chiong, M.C., Chew, K.W., Chen, C.C., Wu, S.Y., Zhou, D., Kumar, G., & Show, P.L. (2020). Immobilized Chlorella species mixotrophic cultivation at various textile wastewater concentrations. Journal of Water Process Engineering, 38, 101609.
Zhuang, L.L., Wang, J.H., & Hu, H.Y. (2018). Differences between attached and suspended microalgal cells in ssPBR from the perspective of physiological properties. Journal of Photochemistry and Photobiology B: Biology, 181, 164–1
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