Extraction and Physicochemical Characterization of Thunbergia laurifolia Lindl. leaf Pectin
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Abstract
The purpose of this research was to study pectin extracted from dried Thunbergia laurifolia Lindl leaves with difference acid type solution i.e. acetic acid, hydrochloric acid, nitric acid and sulfuric acid) adjusted pH 3. Pectin extracted from dried leaves had an extraction percentage in the range of 0.15±0.07 - 0.33±0.16%. The pectin extract employing sulfuric acid solution adjusted pH 3 had the highest amounts of galacturonic acid, esterification percentage, and methoxyl, measuring 52.86±1.72 mg/g, 32.64±1.20%, and 5.40±0.07, respectively, while commercial pectin had the value of 91.27±0.73 mg/g. 45.07±0.26 and 7.35±0.04%, respectively. These results indicated that our pectin belonged to the group of low levels of esterification and methoxyl pectin as well as the extracted pectin was analyzed by Fourier Transform Infrared spectroscopy, which revealed the present of –OH –CH –COOH –COOCH3 and –COO- functional groups corresponding to the commercial pectin. Therefore, it was concluded that the substances extracted from dried T. laurifolia leaves using various acids at pH 3 was pectin.
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References
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