Image analysis reveals cellular fragmentation of Spirulina platensis upon treatment with heavy metal ions

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Lekha Kaushik
Dinakari Sarangan
Deenadayalan Karaiyagowder Govindarajan
Sowmiya Muthumanickam
Muthusaravanan Sivaramakrishnan
Ram Kothandan
Suganthy Masanamani
Saraswathy Nachimuthu
Hadas Mamane
Kumaravel Kandaswamy

Abstract

Bioremediation is a promising technique that can be used to decrease the environmental discharge of Heavy Metal (HM) such as copper, and zinc. Although studies have addressed the cytotoxic effects of HMs, however, the effect of HMs on the geometry of bacterial species such as Spirulina platensis (S. platensis) remains unknown. Quantitative analysis of parameters such as cell perimeter, cell count, and cell distribution shall greatly improve the efficiency of environmental monitoring. Therefore, this study demonstrates the use of an open-source image analysis tool (ImageJ/Fiji) to quantify the aforementioned parameters to analyze the extent of damage caused by lethal concentration of > 2 mg/L of Cu2+ and > 6 mg/L of Zn2+ which disintegrates S. platensis cells into smaller fragments and subsequently affects their structural parameters in perimeter and cell distribution. In summary, this article demonstrates the use of an image analysis platform to quantify the geometric parameters of microbes for environmental monitoring.

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How to Cite
Kaushik, L. ., Sarangan, D. ., Karaiyagowder Govindarajan, D., Muthumanickam, S. ., Sivaramakrishnan, M., Kothandan, R., Masanamani, S. ., Nachimuthu, S., Mamane, H., & Kandaswamy, K. . (2022). Image analysis reveals cellular fragmentation of Spirulina platensis upon treatment with heavy metal ions. Maejo International Journal of Energy and Environmental Communication, 4(1), 49–54. https://doi.org/10.54279/mijeec.v4i1.247678
Section
Research Article