Inhibiting Causal Agent Blast Disease and Growth Promoting of Different Rice Varieties by Bacteria

Abstract

The blast disease outbreaks are a major problem in rice production, caused Pyricularia grisea (Sacc.). The objective of this research is to isolate and efficacy test bacteria to inhibit the mycelium growth of P. grisea and induce seed germination and seedling vigor. A total of 196 bacteria isolates from leaves, root and rhizosphere were separated into 10 groups according to their morphological characteristics on nutrient glucose agar (NGA). The inhibiting mycelium growth of P. grisea strain JM01 by the Dual culture method. The experiment was arranged with a completely randomized design. The results found that the beneficial bacteria strain JMTU02 was most effective in inhibiting the growth of P. grisea, up to 55.72.% (p≤0.05) and in the test of efficiency in inhibiting germ that are infected or contaminated with seeds when compared with chemical control methods (positive control) and autoclave water (negative control). Bacteria strain JMTU02 was still highly effective in promoting germination, plant height, root length, and plant weight of all 4 rice varieties namely RD 15, RD 85, Pathum Thani 1 and Mae Jo 2 between 88-99%, 10.71- 12.33 cm., 1.19-4.64 cm., and plant weight 0.09-0.10 mg., respectively (p≤0.05).