Stability and efficacy determination of crude Limnocharis flava (L.) Buch extract gel in inhibiting pathogenic bacteria
คำสำคัญ:
Limnocharis flava, antibacterial activity, crude extract gel, drug-resistant bacteriaบทคัดย่อ
This study investigated the antibacterial efficacy of crude Limnocharis flava leaf extract gel against antibiotic-resistant clinical bacterial isolates. The gel was formulated with crude ethanolic L. flava leaf extract at 12.5 mg/mL, corresponding to the lowest minimum bactericidal concentration previously reported for the extract. Clinical isolates, including multidrug-resistant Klebsiella pneumoniae (MDR-K), colistin-resistant Pseudomonas aeruginosa (CRP), carbapenem-resistant Enterobacteriaceae (CRE), Proteus mirabilis, and Burkholderia pseudomallei, were used. Antibacterial activity was determined using the agar disc diffusion method. The crude L. flava extract gel inhibited all tested isolates, producing inhibition zones ranging from 8 to 10 mm. MDR-Kwas the most susceptible isolate, showing the largest inhibition zone of 10 mm, followed by CRE with 9 mm. CRP, P. mirabilis, and B. pseudomallei showed comparable inhibition zones of 8 mm. Statistical analysis demonstrated that the activity against MDR-K was significantly higher than that against CRP, P. mirabilis, and B. pseudomallei (p < 0.05), whereas the inhibition observed against CRE showed no significant difference from the other isolates. These results indicate that crude L. flava extract gel exhibits measurable antibacterial activity against clinically relevant antibiotic-resistant bacteria, particularly MDR-K. The moderate inhibition observed may be associated with bioactive phytochemicals in L. flava leaf extract, which may interfere with bacterial growth and viability. Therefore, crude L. flava extract gel represents a potential plant-based antibacterial formulation for further topical pharmaceutical development. Additional studies on phytochemical characterization, formulation stability, cytotoxicity, and antibacterial mechanisms are required.
เอกสารอ้างอิง
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