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The objective of this research was to investigate the optimum ratio for laccase immobilization using barium alginate and comparative paracetamol removal by immobilized laccase in batch reactor and continuous packed bed column reactor with three conditions, paracetamol concentrations, enzyme concentration, pH values. Crude laccase used in this work extracted from Lentinus polychous Lev. was entrapped in sodium alginate and barium chloride at concentration 5% (w/v) and 5% (w/v) respectively. Increasing in the ratio of enzyme to sodium alginate affected an increase in the percentage of immobilization yield and a decrease of enzyme leakage. The optimum ratio of laccase to sodium alginate was 1:8 (v/v). The study of paracetamol removal by immobilized laccase showed the best percentage of paracetamol removals were 78.64% and 100% in batch reactor and continuous packed bed column reactor, respectively at paracetamol concentration 1 mg/L, laccase concentration 0.216 U/g-alginate pH 7 and flow rate at 2 mL/min for continuous packed bed column reactor without the adsorb of paracetamol into entrapment material. The study indicates the potential of immobilized laccase for removal of paracetamol.
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