Optimization of laccase production from Megasporoporia sp. KKU-LKNG-07 using one factor at a time and response surface methodologies

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วิทวัส ทุมแสน
เนตรนภิส ตันเต็มทรัพย์
สุมนา สิริพัฒนากุล-ราษฎร์ภักดี
ภิญญ์ฑิตา มุ่งการดี
โสภณ บุญลือ


This study aims to isolate fungi from Lam Khlong Ngu National Park, Kanchanaburi, Thailand and optimize laccase production. The result showed that three fungal isolates could produce laccase including KKU-LKNG-04,KKU-LKNG-07andKKU-LKNG-16. Based on ITS rRNA gene analysis, these fungi (KKU-LKNG-04,KKU-LKNG-07 andKKU-LKNG-16) were similar toGanoderma lucidumMegasporoporia sp. andRigidopolus vinctus, respectively. The potential fungus isolate which gave the highest lacaase production under solid-state fermentation using sugarcane bagasse as a substate, wasMegasporoporia sp. KKU-LKNG-07.Determination of factors on fungal cultivation related to laccase production was appliedone factor at a time method and optimized by response surface methodology using Box-Behnken approach. The results from one factor at a time method revealed thatconcentration of nitrogen source, pH and temperature obviously influenced fungal cultivation. For optimization of laccase production,the highest laccase production (5.58 U/mL)from fungi Megasporoporia sp. KKU-LKNG-07was predicted under conditions including concentration of nitrogen source, pH and temperature of 4.17 g/L, 8.45 and 29.55 °C, respectively. Later, the laccase production experiment under the optimized conditions was performed. Megasporoporia sp. KKU-LKNG-07 producedlaccase of 5.48 U/mL which was closed to the predicted value.These results indicated the potential of laccase production and optimization which would be useful for further commercial application.

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บทความวิจัย (Research Article)


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