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This study aims to isolate fungi from Lam Khlong Ngu National Park, Kanchanaburi, Thailand and optimize laccase production. The result showed that three fungal isolates could produce laccase including KKU-LKNG-04,KKU-LKNG-07andKKU-LKNG-16. Based on ITS rRNA gene analysis, these fungi (KKU-LKNG-04,KKU-LKNG-07 andKKU-LKNG-16) were similar toGanoderma lucidum, Megasporoporia sp. andRigidopolus vinctus, respectively. The potential fungus isolate which gave the highest lacaase production under solid-state fermentation using sugarcane bagasse as a substate, wasMegasporoporia sp. KKU-LKNG-07.Determination of factors on fungal cultivation related to laccase production was appliedone factor at a time method and optimized by response surface methodology using Box-Behnken approach. The results from one factor at a time method revealed thatconcentration of nitrogen source, pH and temperature obviously influenced fungal cultivation. For optimization of laccase production,the highest laccase production (5.58 U/mL)from fungi Megasporoporia sp. KKU-LKNG-07was predicted under conditions including concentration of nitrogen source, pH and temperature of 4.17 g/L, 8.45 and 29.55 °C, respectively. Later, the laccase production experiment under the optimized conditions was performed. Megasporoporia sp. KKU-LKNG-07 producedlaccase of 5.48 U/mL which was closed to the predicted value.These results indicated the potential of laccase production and optimization which would be useful for further commercial application.
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