Characterization of Manganese Oxide-Biomineralization by the Psychrophilic Marine Bacterium, Arthrobacter sp. Strain NI-2 and Its Spontaneous Mutant Strain NI-2' DOI: 10.32526/ennrj.17.4.2019.32

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Hideki Nakayama
Yusuke Shin
Toru Sumita
Kazuya Urata
Yasuyuki Ikegami


Metal pollution and metal shortage are a growing threat to the global environment and world high-tech industry. One of the promising strategies for removing and recycling metal-elements from the environments is applying Metal-Biotechnology based on metal-related biological activities, which include bioaccumulation, bioadsorption, and biomineralization. In this study, focusing on Manganese (Mn) pollution, we have isolated and analyzed the Mn(II)-oxidizing marine bacterium, Arthrobacter sp. NI-2 strain, from Imari Bay, Imari-shi, Saga, Japan. We have also isolated a spontaneous mutant, Arthrobacter sp. NI-2' strain with enhanced Mn(II)-oxidizing activity. Under the liquid culture condition at 30 °C, Arthrobacter sp. NI-2' strain could efficiently remove more than 96% of Mn(II) from the liquid culture media containing 0.4 mM Mn(II). Although Mn(II)-oxidizing activity of Arthrobacter sp. NI-2 strain is suppressed under the low temperature conditions, the increased Mn(II)-oxidizing activity of Arthrobacter sp. NI-2' strain was maintained when the growth temperature was shifted from 30 °C to 10 °C. Therefore, the Arthrobacter sp. NI-2' strain would be useful as a tool for Mn removal from low-temperature water, such as groundwater around the mining area.


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Nakayama, H., Shin, Y., Sumita, T., Urata, K., & Ikegami, Y. (2019). Characterization of Manganese Oxide-Biomineralization by the Psychrophilic Marine Bacterium, Arthrobacter sp. Strain NI-2 and Its Spontaneous Mutant Strain NI-2’: DOI: 10.32526/ennrj.17.4.2019.32. Environment and Natural Resources Journal, 17(4), 68-77. Retrieved from
Original Research Articles


1. Barboza NR, Guerra‐Sá R, Leão VA. Mechanisms of manganese bioremediation by microbes: An overview. Journal of Chemical Technology and Biotechnology 2016;91:2733-9.

2. Boogerd FC, de Vrind JP. Manganese oxidation by Leptothrix discophora. Journal of Bacteriology 1987; 169(2):489-94.

3. Chang H-W, Bae J-W, Nam Y-D, Kwon H-Y, Park JR, Shin K-S, Kim K-H, Quan Z-X, Rhee S-K, An K-G, Park Y-H. Arthrobacter subterraneus sp. nov., isolated from deep subsurface water of the South Coast of Korea. Journal of Microbiology and Biotechnology 2011;17(11):1875-9.

4. de Vrind JPM, de Vrind-de Jong EW, de Voogt J-WH, Westbroek P, Boogerd FC, Rosson RA. Manganese oxidation by spores and spore coats of a marine Bacillus species. Applied and Environmental Microbiology 1986;52(5):1096-100.

5. Francis CA, Tebo BM. Enzymatic manganese(II) oxidation by metabolically dormant spores of diverse Bacillus species. Applied and Environmental Microbiology 2002;68(2):874-80.

6. Gartemann KH, Eichenlaub R. Isolation and characterization of IS1409, an insertion element of 4-chlorobenzoate-degrading Arthrobacter sp. strain TM1, and development of a system for transposon mutagenesis. Journal of Bacteriology 2001;183(12): 3729-36.

7. Ike M, Yamashita M, Soda S. Handbook of Metal Biotechnology: Applications for Environmental Conservation and Sustainability. Singapore: Pan Stanford Publishing Pte. Ltd; 2011.

8. Krumbein W, Altmann H. A new method for the detection and enumeration of manganese oxidizing and reducing microorganisms. Helgoland Marine Research 1973;25: 347-56.

9. Liang J, Bai Y, Hu C, Qu J. Cooperative Mn(II)-oxidation between two bacterial strains in an aquatic environment. Water Research 2016;89:252-60.

10. Nakayama H, Ikegami Y. Isolation of a manganese oxidizing bacterium, Bacillus sp. NI-1, as a tool for metal recovery from concentrated seawater. Ocean Thermal Energy Conversion 2009;14:65-7.

11. Namgung S, Chon C-M, Lee G. Formation of diverse Mn oxides: A review of bio/geochemical processes of Mn oxidation. Geosciences Journal 2018;22(2):373-81.

12. Nealson KH, Tebo BM, Rosson RA. Occurrence and mechanisms of microbial oxidation of manganese. Advances in Applied Microbiology 1988;33:279-318.

13. Remucal CK, Ginder-Vogel MA. Critical review of the reactivity of manganese oxides with organic contaminants. Environmental Science: Processes and Impacts 2014;16(6):1247-66.

14. Tebo BM, Bargar JR, Clement BG, Dick GJ, Murray KJ, Parker D, Verity R, Webb SM. Biogenic manganese oxides: Properties and mechanisms of formation. Annual Review of Earth and Planetary Science 2004;32:287-328.

15. Zhang H, Li Y, Chen X, Sheng H, An L. Optimization of electroporation conditions for Arthrobacter with plasmid PART2. Journal of Microbiological Methods 2011;84(1):114-20.