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One of the key compounds engaged in carbon dioxide fixation cycle (Calvin-Benson-Bassham cycle) is Ribulose 1, 5- BisphosphateCarboxylase/Oxygenase (RuBisCo). These acts as a carbon sink thus leading to decrease of carbon level. This one of a kind property of RuBisCo thus can help in diminishing an Earth-wide global warming, a noteworthy risk in the present world. In the present study, presence of RuBisCo in Sulfobacillus isolated from Neyveli lignite mines was explored. The growth factors such as pH, Temperature, aeration and light source were optimized for the growth of Sulfobacillus acidophilus strain NY-1. The ideal pH and temperature for Sulfobacillus was seen at pH 1.7 and at 45oC respectively. The cell count was greatest under light condition of 1.60 x 106 at the 60th and 2.96 X 106 in the presence of CO2 with aeration. The presence of RuBisCo under optimized conditions was affirmed by a simple ion exchange chromatography technique.
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