Bioaccumulation of Lead by Pepper Elder (Peperomia pellucida (L.) Kunth) in a Lead-Contaminated Hydroponic System 10.32526/ennrj/19/2021010

Main Article Content

Jessica O. Tablang
Florenda B. Temanel
Ron Patrick C. Campos
Helen C. Ramos

Abstract

Lead (Pb) has become one of the most common heavy metal contaminants, demanding research on economical remediation approaches with minimal ecological impacts. Pepper elder (Peperomia pellucida) is a fast-growing plant that can be a candidate for bioaccumulation and phytoremediation. In this study, the lead bioaccumulation of P. pellucida was assessed by determining the growth response and absorptive capacity of the plant. Plants were grown in hydroponic solution spiked with 500 mg/L of Pb for 28 days. Growth response, absorptive capacity and tolerance of plants grown in contaminated nutrient solution were determined in comparison with control plants. After 28 days of exposure, lead phytotoxicity symptoms such as wilting, chlorosis and necrosis were observed on some plants. The control plants recorded 3.08 g total dry weight (DW) compared to the 1.35 g in Pb-contaminated plants. The tolerance index (TI) of P. pellucida was at 43.40%. The plants were able to absorb lead, with the concentration of lead in the roots (158.6 µg/g) being greater than the concentration of the metal  in the shoots (43.2 µg/g). Meanwhile, bioconcentration factor (BCF) and translocation factor (TF) values were recorded at 0.40 and 0.27, respectively. BCF criterion indicates that the plant is not suitable for phytoextraction, but TF value shows that the plant can be a potential excluder. The findings of the study show that P. pellucida accumulated considerable amount of lead within its tissues, indicating that the plants may be further exploited for their capacity to absorb heavy metals by tweaking several factors that may affect its bioaccumulation ability.

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How to Cite
Tablang, J. O., Temanel, F. B., Campos, R. P. C., & Ramos, H. C. (2021). Bioaccumulation of Lead by Pepper Elder (Peperomia pellucida (L.) Kunth) in a Lead-Contaminated Hydroponic System: 10.32526/ennrj/19/2021010. Environment and Natural Resources Journal, 19(4), 282-291. Retrieved from https://ph02.tci-thaijo.org/index.php/ennrj/article/view/243026
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Original Research Articles

References

1. Aini Syuhaida AW, Norkhadijah SIS, Praveena SM, Suriyani A. The comparison of phytoremediation abilities of water mimosa and water hyacinth. ARPN Journal of Science and Technology 2014;4:722-31.

2. Ali H, Naseer M, Sajad MA. Phytoremediation of heavy metals by Trifolium alexandrinum. International Journal of Environmental Science 2013;2:1459-69.

3. Anoliefo G, Ikhajiagbe B, Okonofhua B, Diafe F. Eco-taxonomic distribution of plant species around motor mechanic workshops in Asaba and Benin City, Nigeria: Identification of oil tolerant plant species. African Journal Of Biotechnology 2006;5(19):1757-62.

4. Arquion RD, Galanida CC, Villamor B, Aguilar HT. Ethnobotanical study of indigenous plants used by local people of Agusan del Sur, Philippines. Asian Pacific Higher Education Journal 2015;2(2):1-11.

5. Atayese MO, Eigbadon AI, Oluwa KA, Adesodun JK. Heavy metal contamination of amaranthus grown along major highways in Lagos, Nigeria. African Crop Science Journal 2009;6:135-225.

6. Belonias BS. Bioaccumulation of lead in the medicinal plant Peperomia pellucida (L.) Kunth. Philippine Journal of Crop Science 2009;34(1):115.

7. Calawagan KM, Japitana JJ, Lapada JA, Alvior HB. Phytoremediation potentials of carabao grass (Paspalum conjugatum), talahib (Saccharum spontaneum), and ulasimang bato (Peperomia pellucida) in removing copper (Cu), lead (Pb), and manganese (Mn) in soils. Proceedings of the PSSN 13th National Scientific Meeting; 2013 May 21-25; Cebu Business Hotel, Cebu City: Philippines; 2013.

8. De Guzman CC. Hydroponic culture of pansit-pansitan (Peperomia pellucida). Proceedings of the 1998 Regional Research and Development Symposia, Philippine Council for Agriculture, Forestry and Natural Resources Research and Development; University of the Philippines Los Baños, Los Baños, Laguna: Philippines; 1999. p. 120.

9. Furini A. Plants and Heavy Metals. Dordrecht, Netherlands: Springer; 2012.

10. Glime JM. Water relations: Conducting structures. In: Glime JM, editor. Bryophyte Ecology (Volume 1). USA: Michigan Technological University and the International Association of Bryologists; 2017. p. 1-26.

11. Gothberg A, Greger M, Holm K, Bengtsson BE. Influence of nutrient levels on uptake and effects of mercury, cadmium, and lead in water spinach. Journal of Environmental Quality 2004;33(4):1247-55.

12. Herlina L, Widianarko B, Sunoko HR. Phytoremediation potential of Cordyline fruticose for lead contaminated soil. Jurnal Pendidikan IPA Indonesia 2020;9(1):42-9.

13. Jaja ET, Odoemena CSI. Effect of Pb, Cu, and Fe compounds on the germination and early seedling growth of tomato varieties. Journal of Applied Sciences and Environmental Management 2004;8(2):51-3.

14. Karimi R, Fitzgerald TP, Fisher NS. A quantitative synthesis of mercury in commercial seafood and implications for exposure in the United States. Environmental Health Perspective 2012;120:1512-9.

15. Kirk JL, Klirnomos JN, Lee H, Trevors JT. Phytotoxicity assay to assess plant species for phytoremediation of petroleum-contaminated soil. Bioremediation Journal 2002;6:57-63.

16. Lado LR, Hengl T, Reuter HI. Heavy metals in European soils: A geostatistical analysis of the FOREGS Geochemical database. Geoderma 2008;148:189-99.

17. Liu S, Xia X, Shen M, Liu R. Polycyclic aromatic hydrocarbons in urban soils of different land uses in Beijing, China: Distribution, sources and their correlation with the city’s urbanization history. Journal of Hazardous Materials 2010;177:1085-92.

18. Liu D, Li S, Islam E, Chen JR, Wu JS, Ye ZQ, et al. Lead accumulation and tolerance of Moso bamboo (Phyllostachys pubescens) seedlings: Applications of phytoremediation. Journal of Zhejiang University Science B 2015;16(2):123-30.

19. Małachowska-Jutsz A, Gnida A. Mechanisms of stress avoidance and tolerance by plants used in phytoremediation of heavy metals. Archives of Environmental Protection 2015;41(4):96-103.

20. Mandkini LL, Bandara NG, Gunawardana D. A Study on the phytoremediation potential of Azolla pinnata under laboratory conditions. Journal of Tropical Forestry and Environment 2016;6(1):36-49.

21. McIntyre T. Phytoremediation of heavy metals from soils. Advances in Biochemical Engineering/Biotechnology 2003;78:97-123.

22. Meeinkuirt W, Pokethitiyook P, Kruatrachue M, Tanhan P, Chaiyarat R. Phytostabilization of a Pb-contaminated mine tailing by various tree species in pot and field trial experiments. International Journal of Phytoremediation 2012;14:925-38.

23. Mirsal IA. Soil Pollution, Origin, Monitoring and Remediation. Berlin, Heidelberg, Germany: Springer; 2004.

24. Mleczek M, Rutkowski P, Kaniuczak J, Szostek M, Budka A, Magdziak Z, et al. The significance of selected tree species age in their efficiency in elements phytoextraction from wastes mixture. International Journal of Environmental Science and Technology 2019;16:3579-94.

25. Mojiri A. The potential of corn (Zea mays) for phytoremediation of soil contaminated with cadmium and lead. Journal of Biological and Environmental Science 2011;5(13):17-22.

26. Mosango DM. Peperomia pellucida (L.) Kunth. In: Schmelzer GH, Gurib-Fakim A, editors. Prota 11(1): Medicinal Plants/Plantes médicinales 1. Wageningen, Netherlands: PROTA; 2008.

27. Napoli M, Cecchi S, Grassi C, Baldi A, Zanchi CA, Orlandini S. Phytoextraction of copper from a contaminated soil using arable and vegetable crops. Chemosphere 2020;219:122-9.

28. Nas FS, Ali M. The effect of lead on plants in terms of growing and biochemical parameters: A review. MOJ Ecology and Environmental Sciences 2018;3(4):265-8.

29. Niu ZX, Sun LN, Sun TH, Li YS, Hong W. Evaluation of phytoextracting cadmium and lead by sunflower, ricinus, alfalfa, and mustard in hydroponic culture. Journal of Environmental Science 2007;19:961-7.

30. Ona LF, Alberto AP, Prudente JA, Sigua GC. Levels of lead in urban soils from selected cities in the rice-based region of the Philippines. Environmental Science and Pollution Research 2006;13(3):177-83.

31. Padmavathiamma PK, Li LY. Phytoremediation technology: Hyperaccumulation metals in plants. Water, Air and Soil Pollution 2007;184:105-26.

32. Putra RS, Novarita D, Cahyana F. Remediation of lead (Pb) and copper (Cu) using water hyacinth (Eichornia crassipes (Mart.) Solms) with electro-assisted phytoremediation (EAPR). AIP Conference Proceedings 2016;1744:020052.

33. Raghavendra HL, Kekuda TRP. Ethnobotanical uses, phytochemistry and pharmacological activities of Peperomia pellucida (L.) Kunth (Piperaceae): A review. International Journal of Pharmacy and Pharmaceutical Sciences 2018; 10(2):1-8.

34. Ramana S, Tripathi AK, Kumar A, Dey P, Saha JK, Patra AK. Phytoremediation of soils contaminated with cadmium by Agave americana. Journal of Natural Fibers 2021;In press:1-9.

35. Salt DE, Smith RD, Raskin I. Phytomediaion. Annual Review of Plant Physiology and Plant Molecular Biology 1998;49:643-68.

36. Santos PJA, Ocampo ETM. SNAP hydroponics: Development and potential for urban vegetable production. Philippine Journal of Crop Science 2005;30(2):3-11.

37. Sharma P, Dubey RS. Lead toxicity in plants. Brazilian Journal of Plant Physiology 2005;17:1-19.

38. Solidum JN, Dahilig VRA, Omran A. Lead levels of water sources in Manila, Philippines. Annals of Faculty Engineering Hunedoara - International Journal of Engineering 2010;8:111-8.

39. Tablang JO, Campos RC, Jacob JS. Phytochemical screening and antibacterial properties of silverbush (Peperomia pellucida) against selected cultured bacteria. Global Journal of Medicinal Plant Research 2020;8(1):1-6.

40. Tanhan P, Kruatrachue M, Pokethitiyook P, Chaiyarat R. Uptake and accumulation of cadmium, lead and zinc by Siam weed (Chromolaena odorata (L.) King and Robinson). Chemosphere 2007;68:323-9.

41. Thayaparan M, Iqbal SS, Chathuranga PK, Iqbal MC. Rhizofiltration of Pb by Azolla pinnata. International Journal of Environmental Sciences 2013;3(6):1811-21.

42. Tolentino RD, Tomas VCB, Travezonda JC, Magnaye BP. Herbal medicine utilization among Batangueños. Asian Pacific Journal of Education, Arts and Sciences 2019;6(1):9-22.

43. United States of America Environmental Protection Agency (USEPA). Selection of Control Technologies for Remediation of Lead Battery Recycling Sites: EPA/540/S-92/011. Washington, DC: Office of Emergency and Remedial Response; 1992.

44. United States of America Environmental Protection Agency (USEPA). Method 7000B Flame Atomic Absorption Spectrophotometry, United States of America Environmental Protection Agency. Washington, DC: Office of Emergency and Remedial Response; 2007.

45. Vamerali T, Bandiera M, Mosca G. Field crops for phytoremediation of metal-contaminated land: A review. Environmental Chemistry Letters 2010;8:1-17.

46. Wang Y, Yang X, Zhang X, Dong L, Zhang J, Wei Y, et al. Improved plant growth and Zn accumulation in grains of rice (Oryza sativa L.) by inoculation of endophytic microbes isolated from a Zn Hyperaccumulator, Sedum alfredii H. Journal of Agricultural Food Chemistry 2014;62:1783-91.

47. Wilson B, Pyatt FN. Heavy metal bioaccumulation by the important food plant, Olea europaea L., in an ancient metalliferous polluted area of Cyprus. Bulletin of Environmental Contamination and Toxicology 2007;78:390-4.

48. Yaowakhan P, Kruatrachue M, Pokethitiyook P, Soonthornsarathool V. Removal of lead using some aquatic macrophytes. Bulletin of Environmental Contamination and Toxicology 2005;75:723-30.

49. Zhivotovsky O, Kuzovkina Y, Schulthess C, Morris CT, Pettinelli D. Lead uptake and translocation by willows in pot and field experiments. International Journal of Phytoremediation 2011; 13:731-49.