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Abstract—The effects of IBA and NAA on rooting and shooting of the Bitter leaf tree (Gymnanthemum extensum) cutting stems were investigated. The stems were obtained from a private garden in Chiang Mai Province. The 25-cm long cutting stems were dipped into distilled water (control), IBA, and NAA with 250, 500, 1000, 2000, and 3000 ppm concentrations for 30 minutes and put in plastic cups in the plant nursery (Program of Biotechnology, Faculty of Science, Maejo University, Chiang Mai) from April to May of 2019. The experiment was performed in a completely randomized design (CRD). The data was collected 30 days after treatments. The result showed that the cutting stem treated with 0ppm (control), 250 ppm of IBA, and 500ppm of IBA gave rise to the highest percentage of rooting (100%). In contrast, the cutting stems treated with IBA at 1,000 and 2,000 ppm concentrations yielded 80% of rooting. NAA at 250, 500, 1,000, and 2,000ppm concentrations gave rise to 73, 73, 73, and 66.7% rooting, respectively. On the other hand, 3000 ppm of NAA provided the lowest percentage of rooting at 53.3%, respectively. Two hundred and fifty ppm of NAA gave the highest average number of roots per cutting compared to the other treatments. The average root length and average root width of control and all treatments were not significantly different (P<0.5). The control, IBA, and NAA at 250, 500, 1,000, and 2,000 ppm concentrations gave the highest percentage of shooting (93 - 100%).
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