In vitro antibacterial activity of Polyalthia debilis (Pierre) Finet & Gagnep Extracts against Klebsiella pneumoniae TISTR 1383
Keywords:
Polyalthia debilis Extracts, antibacterial activity, Klebsiella pneumoniae, MIC, MBCAbstract
This research aimed to determine the anti-Klebsiella pneumoniae activity of Polyalthia debilis (Pierre) Finet & Gagnep extracted with methanol, ethyl acetate, hexane, ethanol, and dichloromethane. The primary screening of anti-Klebsiella pneumoniae activity was tested by the Agar disc diffusion method, and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by the broth microdilution method. The results of the agar disc diffusion revealed that the methanolic and ethanolic stem extracts presented the widest diameter of the inhibition zone of 0.7 cm. The stem extracted with methanol and ethanol showed the lowest MIC value of 0.78 mg/mL and the lowest MBC value of 12.5 mg/ml. This report is the first to demonstrate the efficacy of an extract from the P. debilis that can inhibit and destroy K. pneumoniae, a major drug-resistant group that results in the death of an infected patient in the hospital. It is also important information that can be developed into herbal medicine recipes development and applied on an industrial scale to produce drugs used to control infections from K. pneumoniae.
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