Optimization of mimosine extraction using experimental design
Keywords:
Mimosine, Experimental design, Box–Behnken method, Response surface methodology, Natural deep eutectic solventAbstract
Mimosine is free amino acid, mainly found in lead tree (Leucaena leucocephala). The objective of this research was to study the optimization conditions for extraction of mimosine from young leaves of Leucana Leucocephala using aqueous choline chloride solution as a natural deep eutectic solvent (NADES) by the maceration method. The factors affecting the extraction of mimosine from young leaves of Leucana Leucocephala were concentration NADES, pH, time, the volume of NADES and temperature. The Box–Behnken method and response surface methodology (RSM) were used for experimental design. The obtained extracts solution of mimosine, were analyzed by UV Visible spectroscopy at 282 nm. At a NADES concentration of 0.50 molal pH 6 and an extraction time of 90 minutes, the maximum amount of mimosine was 80.03 ± 2.29 mg/g, and at 50.00 ml volume of NADES, pH 7 and temperature of 90 °C, the maximum amount of mimosine was 76.46 ± 0.95 mg/g. Studies have shown that extraction of mimosine from young leaves of Leucana Leucocephala with NADES improves the efficiency of the mimosine extraction than conventional extraction with hot water.
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เนื้อหาและข้อมูลในบทความที่ลงตีพิมพ์ในวารสารวิจัย วิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยราชภัฏนครราชสีมา ถือเป็นข้อคิดเห็นและความรับผิดชอบของผู้เขียนบทความโดยตรงซึ่งกองบรรณาธิการวารสาร ไม่จำเป็นต้องเห็นด้วย หรือร่วมรับผิดชอบใด ๆ
บทความ ข้อมูล เนื้อหา รูปภาพ ฯลฯ ที่ได้รับการตีพิมพ์ในวารสารวิจัย วิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยราชภัฏนครราชสีมา ถือเป็นลิขสิทธิ์ของวารสารวิจัย วิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยราชภัฏนครราชสีมา หากบุคคลหรือหน่วยงานใดต้องการนำทั้งหมดหรือส่วนหนึ่งส่วนใดไปเผยแพร่ต่อหรือเพื่อกระทำการใด ๆ จะต้องได้รับอนุญาตเป็นลายลักอักษรจากวารสารวิจัย วิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยราชภัฏนครราชสีมา ก่อนเท่านั้น
