Screening of Bacillus sp. as Plant Growth-Promoting Bacteria
Keywords:
plant growth promoting rhizobacteria, screening, bacillusAbstract
The effective bacteria were isolated from soil samples in the central and eastern regions of Thailand. A total of 260 bacterial isolates were collected, of which 30 were spore-forming bacteria. These spore-forming bacteria were tested for hemolytic activity to assess preliminary safety and tested further based on plant growth-promoting abilities such as phosphate solubilization, plant pathogen inhibition (against Erwinia carotovora, Ralstonia solanacearum, Xanthomonas axonopodis pv. citri, and X. oryzae pv. oryzae) and plant hormone production (indole acetic acid and gibberellic acid). Results showed that all isolated strains exhibited different abilities of plant growth-promoting traits. Thus, the potential PGPB strains were selected and identified based on 16S rDNA sequence. Nine effective isolated strains consisting of A8, A21, Bb_4.2, Bc_2.1, Bc_6.1, BS2_1.2, BC2_3.2, BZ2_5.1, and BB2_4.3 were identified as Bacillus zanthoxyli, B. velezensis, B. tequilensis, B. infantis, B. infantis, B. licheniformis, B. wiedmannii, B. altitudinis and B. aryabhattai, respectively.
References
Abuhena, M., Al-Rashid, J., Azim, M.F., Khan, M.N.M., Kabir, M.G., Barman, N.C., Rasul, N.M., Akter, S., & Huq, M.A. (2022). Optimization of industrial (3000L) production of Bacillus subtilis CW-S and its novel application for minituber and industrial-grade potato cultivation. Scientific Reports, 12, 11153. https://doi.org/10.1038/s41598-022-15366-5
Ambrosini, A., & Passaglia, L.M.P. (2017). Plant Growth-Promoting Bacteria (PGPB): Isolation and screening of PGP Activities. Current protocols in plant biology, 2(3), 190–209. https://doi.org/10.1002/pb.20054
Anguiano, C.J.C., Flores, O.A., Olalde, P.V., Arredondo, V.R., & Laredo, A.E.I. (2019). Evaluation of Bacillus subtilis as promoters of plant growth. Revista Bio Ciencias, 6, e418. https://doi.org/10.15741/revbio.06.e418
Balderas-Ruíz, K. A., Gómez-Guerrero, C. I., Trujillo-Roldán, M. A., Valdez-Cruz, N. A., Aranda-Ocampo, S., Juárez, A. M., Leyva, E., Galindo, E., & Serrano-Carreón, L. (2021). Bacillus velezensis 83 increases productivity and quality of tomato (Solanum lycopersicum L.): Pre and postharvest assessment. Current research in microbial sciences, 2, 100076. https://doi.org/10.1016/j.crmicr.2021.100076
Balouiri, M., Sadiki, M., & Ibnsouda, S.K. (2016). Methods for in vitro evaluating antimicrobial activity: A review. Journal of pharmaceutical analysis, 6, 71–79. https://doi.org/10.1016/j.jpha.2015.11.005
Barbaccia, P., Gaglio, R., Dazzi, C., Miceli, C., Bella, P., Lo Papa, G., & Settanni, L. (2022). Plant growth-promoting activities of bacteria isolated from an anthropogenic soil located in Agrigento province. Microorganisms, 10(11), 2167. https://doi.org/10.3390/microorganisms10112167
Chowhan, L. B., Mir, M. I., Sabra, M. A., El-Habbab, A. A., & Kiran-Kumar, B. (2023). Plant growth promoting and antagonistic traits of bacteria isolated from forest soil samples. Iranian journal of microbiology, 15(2), 278–289. https://doi.org/10.18502/ijm.v15i2.12480
Dobrzynski, J., Jakubowska, Z., & Dybek, B. (2022). Potential of Bacillus pumilus to directly promote plant growth. Frontiers in microbiology, 13, 1069053. https://doi.org/10.3389/fmicb.2022.1069053
Glick, R.B. (2012). Plant growth-promoting bacteria: Mechanisms and applications. Scientifica, 2012, 963410. https://onlinelibrary.wiley.com/doi/epdf/10.6064/2012/963401
Gratao, P.L., Prasad, M.N.V., Cardoso, P.F., Lea, P.J., & Azevedo R.A. (2005). Phytoremediation: green technology for the cleanup of toxic metals in the environment. Brazilian Journal of Plant Physiology, 17, 53-64. https://doi.org/10.1590/S1677-04202005000100005
Hashem, A., Tabassum, B., & Fathi Abd-Allah, E. (2019). Bacillus subtilis: A plant growth- promoting rhizobacterium that also impacts biotic stress. Saudi Journal of Biological Sciences, 26(6), 1291-1297. https://doi.org/10.1016/j.sjbs.2019.05.004
Jetiyanon, K., & Kloepper, J.W. (2002). Mixtures of plant growth-promoting rhizobacteria for induction of systemic resistance against multiple plant diseases. Biological Control, 24, 285-291. http://dx.doi.org/10.1016/S1049-9644(02)00022-1
Katsenios, N., Andreou, V., Sparangis, P., Djordjevic, N., Giannoglou, M., Chanioti, S., Stergiou, P., Xanthou, M. Z., Kakabouki, I., Vlachakis, D., Djordjevic, S., Katsaros, G., & Efthimiadou, A. (2021). Evaluation of plant growth promoting bacteria strains on growth, yield and quality of industrial tomato. Microorganisms, 9(10), 2099. https://doi.org/10.3390/microorganisms9102099
Li, Y., You, X., Tang, Z., Zhu, T., Liu, B., Chen, M.X., Xu, Y., & Liu, T.Y. (2022) Isolation and identification of plant growth-promoting rhizobacteria from tall fescue rhizosphere and their functions under salt stress. Physiologia Plantarum, 174(6), e13817. https://doi.org/10.1111/ppl.13817
Mogrovejo, D.C., Perini, L., Gostincar, C., Sepcic, K., Turk, M., Ambrozic-Avgustin, J., & Brill, F.H.H. (2020). Prevalence of antimicrobial resistance and hemolytic phenotypes in culturable arctic bacteria. Frontiers in Microbiology, 11. https://doi.org/10.3389/fmicb.2020.00570.
Mun, B.G., Hussain, A., Park, Y.G., Kang, S.M., Lee, I.J., & Yun, B.W. (2024). The PGPR Bacillus aryabhattai promotes soybean growth via nutrient and chlorophyll maintenance and the production of butanoic acid. Frontiers in Plant Science, 15. https://doi.org/10.3389/fpls.2024.1341993
Nautiyal, C.S. (1999). An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiology Letters, 170, 265-270. https://doi.org/10.1111/j.1574-6968.1999.tb13383.x
Poria, V., Debiec-Andrzejewska, K., Fiodor, A., Lyzohub, M., Ajijah, N., Singh, S., & Pranaw, K. (2022). Plant Growth-Promoting Bacteria (PGPB) integrated phytotechnology: A sustainable approach for remediation of marginal lands. Frontiers in Plant Science, 13. https://doi.org/10.3389/fpls.2022.999866
Pranaw, K., Pidlisnyuk, V., Trögl, J., & Malinská, H. (2020). Bioprospecting of a novel plant growth-promoting bacterium Bacillus altitudinis KP-14 for enhancing Miscanthus × giganteus growth in metals contaminated soil. Biology, 9(9), 305. https://doi.org/10.3390/biology9090305
Saad, M.M.G., Kandil, M., & Mohammed, Y.M.M. (2020). Isolation and identification of plant growth-promoting bacteria highly effective in suppressing root rot in Fava beans.Curr Microbiol. 77, 2155–2165. https://doi.org/10.1007/s00284-020-02015-1
Saeid, A., Prochownik, E., & Dobrowolska-Iwanek, J. (2018). Phosphorus solubilization by Bacillus species. Molecules (Basel, Switzerland), 23(11), 2897. https://doi.org/10.3390/molecules23112897
Tsotetsi, T., Nephali, L., Malebe, M., & Tugizimana, F. (2022). Bacillus for plant growth promotion and stress resilience: What have we learned? Plants (Basel, Switzerland), 11(19), 2482. https://doi.org/10.3390/plants11192482
Vásquez, E., & Carlos, M. (2023). Isolation and identification of bacteria of genus Bacillus from composting urban solid waste and palm forest in Northern Peru. Microorganisms, 11, 751. https://doi.org/10.3390/microorganisms1103075
Zhou, L., Song, C., Li, Z., & Kuipers, O.P. (2021). Antimicrobial activity screening of rhizosphere soil bacteria from tomato and genome-based analysis of their antimicrobial biosynthetic potential. BMC Genomics, 22. https://doi.org/10.1186/s12864-020-07346-8
Downloads
Published
Versions
- 2025-02-24 (2)
- 2024-06-27 (1)
How to Cite
Issue
Section
License
Copyright (c) 2024 Faculty of Liberal Art and Science, Roi-Ed Rajabhat University
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
บทความที่ได้รับการตีพิมพ์เป็นลิขสิทธิ์ของคณะศิลปศาสตร์และวิทยาศาสตร์ มหาวิทยาลัยราชภัฏร้อยเอ็ด
ข้อความที่ปรากฏในบทความแต่ละเรื่องในวารสารวิชาการเล่มนี้เป็นความคิดเห็นส่วนตัวของผู้เขียนแต่ละท่านไม่เกี่ยวข้องกับมหาวิทยาลัยราชภัฎร้อยเอ็ด และคณาจารย์ท่านอื่นๆในมหาวิทยาลัยฯ แต่อย่างใด ความรับผิดชอบองค์ประกอบทั้งหมดของบทความแต่ละเรื่องเป็นของผู้เขียนแต่ละท่าน หากมีความผิดพลาดใดๆ ผู้เขียนแต่ละท่านจะรับผิดชอบบทความของตนเองแต่ผู้เดียว
