Survey of Escherichia coli contamination found in ready-to-drink beverages and ice at Loei Rajabhat University's restaurant
Keywords:
Escherichia coli, contamination, ready-to-drink beverages, iceAbstract
The purpose of this study was to investigate Escherichia coli contamination in ready-to-drink beverages and ice at Loei Rajabhat Cafeteria. Seventy-two samples were collected and evaluated using the 3-tube Most Probable Number (MPN) method and validated by the IMViC biochemical test. The results showed that ready-to-drink beverages and ice samples were contaminated with E. coli at levels ranging from < 3.0 to 23.0 MPN/mL. Twenty-nine samples (40.28%) exhibited E. coli contamination above Notification of Department of Medical Sciences: the microbiological quality criteria for food and food contact containers in Volume 3 (2017) issued by Ministry of Public Health of Thailand. The established requirements stipulate that beverages contained in non-sealed vessels must not exhibit E. coli in 1 mL of sample. The results were also compared to the Ministry of Public Health's notification No. 78 (1984) for ice, which states that ice for eating must not include E. coli. Thus, this study indicated that ready-to-drink beverages and ice samples from the cafeteria at Loei Rajabhat University were contaminated with a significant amount of E. coli, potentially causing health risks for customers. Better food sanitary standards may be required from food vendors.
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