Efficacy of Bacillus subtilis isolate AGBM for Inhibition of Colletotrichum gloeosporioides (Penz.) Sacc. Causing Chilli Anthracnose
Keywords:
Bacillus subtilis, Anthracnose, ChilliAbstract
The study aimed to examine the antagonistic potential of Bacillus subtilis isolate AGBM for growth and sporulation inhibition of Colletotrichum gloeosporioides causing chilli anthracnose. B. subtilis isolate AGBM (from Agrobiomate Co., Ltd.) compared with B. subtilis isolate from Department of Agriculture and 2 commercial isolates of B. subtilis were tested for against mycelial growth and sporulation of chilli anthracnose pathogen; C. gloeosporioides isolate C14; by bi-culture antagonistic test. Experimental design by Completely Randomized Design (CRD) with 4 replications was used in this study. Results showed that B. subtilis AGBM could inhibit mycelial growth and sporulation of chilli anthracnose pathogen at 85.00 and 76.84%, respectively. It has been proved that B. subtilis has become the promising antagonistic bacteria as a biological agent plant pathogenic fungus.
References
Abdelmoteleb, A., Troncoso-Rojas, R., González-Soto, T., & González-Mendoza, D. (2017). Antifungical activity of autochthonous Bacillus subtilis Isolated from Prosopis juliflora against phytopathogenic fungi. Mycobiology, 45(4), 385-391. https://doi.org/10.5941/myco.2017.45.4.385
Choi, H.W., Hassan, O., Ryu, H., Ju, H.Y., & Hong, S.K. (2024). First report of Colletotrichum gigasporum causing anthracnose of chili pepper in South Korea. Plant disease, 108(9), 2932. https://doi.org/10.1094/pdis-05-24-0945-pdn
Gharieb, M.M., Rizk, A., & Elfeky, N. (2024). Anticandidal activity of a wild Bacillus subtilis NAM against clinical isolates of pathogenic Candida albicans. Annals of Microbiology, 74, Article 23. https://doi.org/10.1186/s13213-024-01764-9
Jiang, C.-M., Li, Z.-Z., Shi, Y., Guo, D., Pang, B., Chen, X., Shao, D., Liu, Y.-L., & Shi, J.-L. (2020). Bacillus subtilis inhibits Aspergillus carbonarius by producing iturin A, which disturbs the transport, energy metabolism, and osmotic pressure of fungal cells as revealed by transcriptomics analysis. International journal of food microbiology, 330, Article 108783. https://doi.org/10.1016/j.ijfoodmicro.2020.108783
Kiesewalter, H. T., Lozano-Andrade, C. N., Wibowo, M., Strube, M. L., Maróti, G., Snyder, D., Jørgensen, T. S., Larsen, T. O., Cooper, V., Weber, T., & Kovács, Á. (2021). Genomic and chemical diversity of Bacillus subtilis secondary metabolites against plant pathogenic fungi. mSystems, 6(1), Article e00770-20. https://doi.org/10.1128/msystems.00770-20
Kumar, A., Rabha, J., & Jha, D.K. (2021). Antagonistic activity of lipopeptide-biosurfactant producing Bacillus subtilis AKP, against Colletotrichum capsici, the causal organism of anthracnose disease of chilli. Biocatalysis and Agricultural Biotechnology, 36, Article 102133. https://doi.org/10.1016/j.bcab.2021.102133
Le Thanh, T., Cong, V. K., Sangpueak, R., Numparditsub, P., Papathoti, N. K., Machikowa, T., & Buensanteai, K. (2023). Efficacy of Bacillus subtilis for controlling anthracnose in chilli. Agriculture and Natural Resources, 57(2), 223-232. https://li01.tci-thaijo.org/index.php/anres/article/view/259080
Oo, M. M., & Oh, S. K. (2016). Chilli anthracnose (Colletotrichum spp.) disease and its management approach. Korean Journal of Agricultural Science, 43(2), 153-162. http://dx.doi.org/10.7744/kjoas.20160018
Pandey, C., Prabha, D., Negi, Y., Maheshwari, D., Dheeman, S., & Gupta, M. (2023). Macrolactin A mediated biocontrol of Fusarium oxysporum and Rhizoctonia solani infestation on Amaranthus hypochondriacus by Bacillus subtilis BS-58. Frontiers in Microbiology, 14. https://doi.org/10.3389/fmicb.2023.1105849
Pathumporn, C., Pimratch, S., & Ratanacherdchai, K. (2024) Efficacy of Trichoderma asperellumfor controlling Colletotrichum gloeosporioides causing anthracnose disease of Mangifera indica L.cv Nam Dok Mai. Prawarun Agricultural Journal, 21(1), 202-209. https://doi.org/10.14456/paj.2024.23
Ratanacherdchai, K., Chaiyarak, T., & Juntachum, N. (2019). Preliminary investigation of Trichoderma asperellum’s culture and extracts against black rot of orchid. Prawarun Agricultural Journal, 16(1), 204 – 211. https://li01.tci-thaijo.org/index.php/pajrmu/article/view/249067/170315
Rattana, T. (2016). Efficiency of pineapple and papaya crude extracts against chilli anthracnosedDisease. Thai Science and Technology Journal, 24(3), 456-468. https://li01.tci-thaijo.org/index.php/tstj/article/view/52971
Saxena, A., Raghuwanshi, R., Gupta, V. K., & Singh, H. B. (2016). Chilli anthracnose: the epidemiology and management. Frontiers in microbiology, 7, Article 1527. https://doi.org/10.3389/fmicb.2016.01527
Somjinda, S., Athinuwat, D., & Chuaboon, W. (2020). Efficacy and identification of beneficial bacteria to control Colletotrichum capsici caused anthracnose of chili. Agriculture and Technology Journal, 1(1), 78-83. https://li01.tci-thaijo.org/index.php/atj/article/view/228942
Sukhawatthanakun, K. (2022). Marketing channels of chili in the Upper Northeastern Region of Thailand. Kasetsart Journal of Social Sciences, 43(3), 637–644. https://doi.org/10.34044/j.kjss.2022.43.3.14
Surawut, S., Kunsook, C., Chaikul, S., & Jaikong, R. (2023). Isolation of bacteria contained in vermicompost with antifungal activity of chili anthracnose. Journal of Science & Science Education, 6(2), 181-191. http://doi.org/10.14456/jsse.2023.16
Thapanapongworakul, P., & Ratanasakchaichan, C. (2016). Efficacy of bio-extracts from chili residues against Colletotrichum gloeosporioides in vitro. Journal of Agricultural Research and Communications, 32(1), 61-72. https://li01.tci-thaijo.org/index.php/joacmu/article/view/245589
Wannawong, S.I., Chompoo, W. & Panngom, K. (2024). Effect of hydrogen peroxide on the control of chili anthracnose caused by Colletotrichum sp. Agriculture and Technology Journal, 5(1), 15-25. https://li01.tci-thaijo.org/index.php/atj/article/view/259393
Xiao, J., Guo, X.-J., Qiao, X., Zhang, X., Chen, X.-M., & Zhang, D.-D. (2021). Activity of fengycin and iturin A isolated from Bacillus subtilis Z-14 on Gaeumannomyces graminis var. tritici and soil microbial diversity. Frontiers in Microbiology, 12, Article 682437. https://doi.org/10.3389/fmicb.2021.682437
Yu, C., Liu, X., Zhang, X., Zhang, M., Gu, Y., Ali, Q., Mohamed, M., Xu, J., Shi, J., Gao, X.-W., Wu, H.-J., & Gu, Q. (2021). Mycosubtilin produced by Bacillus subtilis ATCC6633 Inhibits growth and mycotoxin biosynthesis of Fusarium graminearum and Fusarium verticillioides. Toxins, 13(11), Article 791. https://doi.org/10.3390/toxins13110791
Yutthasin, R., Worasatit, N., Thomma, K, Dumkhum, W., & Seelarak, P. (2018). Selection of potential antagonistic Bacillus species to broad spectrum for control Colletotrichum spp., causal agent of chilli anthracnose disease. Khon Kaen Agriculture Journal, 46 (Suppl. 1), 1130-1136. https://agkaj.kku.ac.th/papers/1765/pdf
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