Study of Chicken Eggshells for the synthesis of Biomedical Materials in Orthopedic Surgery

Main Article Content

kaison harisaeng
Teerawat Laonapakul

Abstract

Tetracalcium phosphate (TTCP) is a calcium phosphate compound with considerable potential for medical applications. This study investigated the use of waste chicken eggshells from restaurants as a precursor for TTCP synthesis. The eggshells were subjected to heat treatment at various temperatures (200, 300, 400, 500, and 600 °C) and subsequently analyzed for their crystal structure and morphology. The findings indicated that eggshells heated at 200 °C were most suitable for this purpose, exhibiting a light brownish-white color and containing calcium carbonate (CaCO3) in the form of calcite. The CaCO3 derived from eggshells was then combined with dicalcium phosphate dihydrate (DCPD) to synthesize TTCP via a solid-state reaction method. This synthesized TTCP was compared with TTCP produced from commercially available CaCO3. The results from XRD and SEM analysis revealed that the synthesized TTCP from both sources possessed similar crystal structures and morphologies. This demonstrates that eggshells can serve as a viable alternative material for TTCP synthesis. The potential applications of this synthesized TTCP include the development of artificial bone structures and bone cement for medical use.

Article Details

How to Cite
1.
harisaeng kaison, Teerawat Laonapakul. Study of Chicken Eggshells for the synthesis of Biomedical Materials in Orthopedic Surgery . featkku [internet]. 2024 Dec. 26 [cited 2026 Feb. 13];10(2):30-4. available from: https://ph02.tci-thaijo.org/index.php/featkku/article/view/255410
Section
Research Articles

References

Otsuka M, Matsuda Y, Suwa Y, Fox JL, Higuchi WI. A novel skeletal drug delivery system using a self‐setting calcium phosphate cement. 5. Drug release behavior from a heterogeneous drug‐loaded cement containing an anticancer drug. J Pharm Sci. 1994; 83(11): 1565–8.

Sugawara A, Fujikawa K, Kusama K, Nishiyama M, Murai S, Takagi S, et al. Histopathologic reaction of a calcium phosphate cement for alveolar ridge augmentation. J Biomed Mater Res. 2002; 61(1): 47–52.

Ambard AJ, Mueninghoff L. Calcium phosphate cement: Review of mechanical and biological properties. J Prosthodont. 2006; 15(5): 321–8.

Jeon C, Chun S, Lim S, and Kim S. Synthesis and Characterization of TTCP for Calcium Phosphate Bone Cement, Biomater. Res., vol. 15, no. 1, pp. 1–6, 2011.

Moseke C, Gbureck U. Tetracalcium phosphate: Synthesis, properties and biomedical applications, Acta Biomater., vol. 6, no. 10, pp. 3815–3823, 2010, doi: 10.1016/j.actbio.2010.04.020.

Komath M, Varma H.K, Sivakumar R. On the development of an apatitic calcium phosphate bone cement, Bull. Mater. Sci., vol. 23, no. 2, pp. 135–140, 2000, doi: 10.1007/BF02706555.

Waheed M. et al., Channelling eggshell waste to valuable and utilizable products: A comprehensive review, Trends Food Sci. Technol., vol. 106, no. October, pp. 78–90, 2020, doi: 10.1016/j.tifs.2020.10.009.

Laohavisuti N, Boonchom B, Boonmee W, Chaiseeda K, Seesanong S. Simple recycling of biowaste eggshells to various calcium phosphates for specific industries, Sci. Rep., vol. 11, no. 1, pp. 1–11, 2021, doi: 10.1038/s41598-021-94643-1.

Quina M.J, Soares M.A.R, Quinta-Ferreira R. Applications of industrial eggshell as a valuable anthropogenic resource, Resour. Conserv. Recycl., vol. 123, pp. 176–186, 2017, doi: 10.1016/j.resconrec.2016.09.027.

Lee S.W, Kim S.G, Balázsi C, Chae W.S, Lee H.O. Comparative study of hydroxyapatite from eggshells and synthetic hydroxyapatite for bone regeneration, Oral Surg. Oral Med. Oral Pathol. Oral Radiol., vol. 113, no. 3, pp. 348–355, 2012, doi: 10.1016/j.tripleo.2011.03.033.

Dwivedi S.P, Sharma S, Mishra R.K. Characterization of waste eggshells and CaCO3 reinforced AA2014 green metal matrix composites: A green approach in the synthesis of composites, Int. J. Precis. Eng. Manuf., vol. 17, no. 10, pp. 1383–1393, 2016, doi: 10.1007/s12541-016-0164-z.

Murakami F.S, Rodrigues P.O, De Campos C.M.T, Silva M.A.S. Physicochemical study of CaCO3 from egg shells, Cienc. e Tecnol. Aliment., vol. 27, no. 3, pp. 658–662, 2007, doi: 10.1590/S0101-20612007000300035.

Siva Rama Krishna D, Siddharthan A, Seshadri S.K, Sampath Kumar T.S. A novel route for synthesis of nanocrystalline hydroxyapatite from eggshell waste, J. Mater. Sci. Mater. Med., vol. 18, no. 9, pp. 1735–1743, 2007, doi: 10.1007/s10856-007-3069-7.

Edyvean R.G.J, Apiwatanapiwat W, Vaithanomsat P, Boondaeng A, Janchai P, Sophonthammaphat S. The Bio-Circular Green Economy model in Thailand – A comparative review, Agric. Nat. Resour., vol. 57, no. 1, pp. 51–64, 2023, doi: 10.34044/j.anres.2023.57.1.06.

Pikul D, Garnjanagoonchorn W, Thanachasai S. The development of eggshell membrane hydrolysate; 2014.

Liang X, Cong H, Jiang G, Rao R.P, He H, Ramakrishna S. Eggshell membrane: Structure, purification, properties and multifunctional applications, Food Biosci., vol. 60, no. March, p. 104487, 2024, doi: 10.1016/j.fbio.2024.104487.

Mensah R.A, Salim K, Peszko K, Diop S, Wong T.H, Chau D.Y. The chicken eggshell membrane: a versatile, sustainable, biological material for translational biomedical applications, Biomed. Mater., vol. 18, no. 4, 2023, doi: 10.1088/1748-605X/acd316.

Kristl M, Jurak S, Brus M, Sem V, Kristl J. Evaluation of calcium carbonate in eggshells using thermal analysis, J. Therm. Anal. Calorim., vol. 138, no. 4, pp. 2751–2758, 2019, doi: 10.1007/s10973-019-08678-8.

Jimoh O.A, Ariffin K.S, Bin Hussin H, Temitope A.E. Synthesis of precipitated calcium carbonate: a review, Carbonates and Evaporites, vol. 33, no. 2, pp. 331–346, 2018, doi: 10.1007/s13146-017-0341-x.

Maleki Dizaj S, Barzegar-Jalali M, Zarrintan M.H, Adibkia K, Lotfipour F. Calcium carbonate nanoparticles as cancer drug delivery system, Expert Opin. Drug Deliv., vol. 12, no. 10, pp. 1649–1660, 2015, doi: 10.1517/17425247.2015.1049530.

Guo D, Xu K, Han Y. Influence of cooling modes on purity of solid-state synthesized tetracalcium phosphate, Mater. Sci. Eng. B, vol. 116, no. 2, pp. 175–181, 2005, doi: 10.1016/j.mseb.2004.09.032.

Ishikawa K, Takagi S, Chow L.C, Suzuki K. Reaction of calcium phosphate cements with different amounts of tetracalcium phosphate and dicalcium phosphate anhydrous, J. Biomed. Mater. Res., vol. 46, no. 4, pp. 504–510, 1999, doi: 10.1002/(SICI)1097-4636(19990915)46:4<504::AID-JBM8>3.0.CO;2-H.