Determination of metal concentration in vitreous humour and synovial fluid using Inductively Coupled Plasma – Mass Spectrometry for estimation of the postmortem interval.
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Abstract
The research objective is to study the change rate of metal concentration in vitreous humour and synovial fluid versus post-mortem interval.
Sodium, Potassium, Calcium, Iron and Copper were the interested elements since there are naturally found in cell body. Samples were collected
from ten carcasses of adult pigs weighted about 80 kg. The experiments were carried out in Thailand during May. The vitreous humour and
synovial fluid were collected in different periods up to 48 hours after the pig died. The quantity of each metal was determined by the inductively
coupled plasma-mass spectroscopy (ICP-MS) technique.
The results showed that the change rate of metal concentrations increased when the post-mortem intervals increased, excepts sodium concentration.
On the other hand, vitreous humour’s copper concentration increases while synovial fluid’s copper concentration decreased with the increased of
post-mortem interval. The correlation is not significantly different between vitreous humour and synovial fluid for all elements. Both biological samples
can be representative and alternatively analysed. Relationships between metal concentrations in vitreous humour and synovial fluid were found to be consistent.
Hence, the estimation of post-mortem interval can be examined from both biological samples.
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