Formulation of plant extracts for some pathogenic bacteria inhibition
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Abstract
Medicinal plants could be an alternative treatment for non-infectious and infectious diseases, especially bacterial skin infections. This study selected 16 plant extracts and prepared 16 recipes to determine anti-bacterial activity by agar disc diffusion method. Primary results of anti-bacterial activity showed inhibitory activity from formulation number 3 (Bengal-Currants), 12 (Betel) and 15 (Galangal) which was significantly different from those of other formulations at p<0.05. Then, the three mixture extracts were prepared for 4 formulas namely formulation 17, 18, 19 and 20. Anti-bacterial activity of these 4 recipes were determined by agar disc diffusion method. The results showed that all of them gave an anti-bacterial activity against Staphylococcus aureus and Escherichia coli. However, after observation at 48 h., only formula 18 completely inhibited tested bacteria around the disc. Formula 18 was selected to investigate the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values against representative bacterial stains by broth microdilution method. The MIC/MBC values for S. aureus E. coli and MRSA as 0.62/0.62, 2.5/5.0 and 0.31/0.31 mg/ml, respectively. Taken together, this study provide evidence that formulations of extract mixture from Bengal-Currants, Betel, and Galangal, has antibacterial activity. These formulations should be developed to control pathogenic bacteria.
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