Effects of Moringa oleifera Lam. seed oil on lipid profiles and bone biomarkers in ovariectomized rats
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Abstract
This research evaluated effects of Moringa oleifera seed oil (MSO) on lipid profiles and bone biomarkers in ovariectomized (OVX) rats. To study the preventive effect and the recovery effect, rats were ovariectomized and fed with MSO on day 3 after ovariectomy (Group 1) and on day 60 after ovariectomy (Group 2), respectively. Each group was then divided into five subgroups; sham-operated control (SHAM); OVX rats treated with vehicle; OVX treated with MSO 0.25 mL/100g BW/day, OVX treated with MSO 0.50 mL/100g BW/day and OVX treated with 17b-estradiol 10 µg/kg BW/day. After 6 weeks of treatment; serum of rats in each treatment group was analyzed to determine lipid profiles [the total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C)] and bone biomarkers [alkaline phosphatase (ALP), serum calcium, serum phosphorus]. The urine samples were also collected to determine urine calcium, phosphorus and creatinine. OVX rats treated with MSO showed significant decreases in LDL-C, increases in HDL-C compared to the OVX-control group. MSO treated rats restored the elevated serum levels of Ca, P and ALP. The oil also decreased urine Ca and P levels in all OVX groups compared to the SHAM group (p < 0.05). Taken together, these findings suggest that MSO intake can improve lipid profiles and bone biomarkers in ovariectomized rats.
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Rosenberg L., Hennekens C. H., Rosner B., Belanger C., Rothman K. J. and Speizer F. E. Early menopause and the risk of myocardial infarction. Am J Obstet Gynecol. 1981. 139(1): 47-51.
Félix-Redondo, F. J., Grau, M. and Fernández-Bergés, D. Cholesterol and Cardiovascular Disease in the Elderly. Facts and Gaps. Aging Dis. 2013. 4(3): 154-169.
Srivastava N., Chowdhury P. R., Averna M. and Srivastava R. A. Estrogen increases hepatic lipase levels in inbred strains of mice: a possible mechanism for estrogen-dependent lowering of high density lipoprotein. Mol Cell Biochem. 2001. 220: 87-93.
Nevin K. G. and Rajamohan T. Beneficial effects of virgin coconut oil on lipid parameters and in vitro LDL oxidation. Clin Biochem. 2004. 37: 830-835.
Boulbaroud S., El-Hessni A., Azzaoui F. Z. and Mesfioui A. Sesame seed oil and flaxseed oil affect plasma lipid levels and biomarkers of bone metabolism in ovariectomized Wistar rats. Biol Med. 2012. 4(3): 102-110.
Tahmasbi S., Heidarpour M. and Jafar A. M. and Mehrjerd, H. K. Effects of pomegranate seed oil on oxidative stress parameters and lipid profiles in ovariectomized rats. Iran J Vet Surg. 2013. 8(2): 17-24.
Mohamed N. E. and Wakwak M. M. Effect of sesame seeds or oil supplementation to the feed on some physiological parameters in Japanese Quail. J Radiat Res Appl Sci. 2014. 7: 101-109.
Corbett, P. It is time for an oil change opportunities for high-oleic vegetables oils. Informs. 2003. 14: 480-481.
Leone A., Spada A., Battezzati A., Schiraldi A., Aristil J. and Bertoli, S. Moringa oleifera Seeds and Oil: Characteristics and Uses for Human Health. Int J Mol Sci. 2016. 17(12): 2141.
Tsaknis J., Lalas S., Gergis V., Dourtoglou V. and Spilitois V. Characterization of Moringa oleifera seed oil (Mbololo variety of Kenya). J Agric Food Chem. 1999. 47: 4495-4499.
Khaled M. and Koriem M. Antihyperlipidemic activity of the medicinal plants among Kadazan and Dusun communities in Sabah, Malaysia: a review. Asia Pac J Trop Biomed. 2014. 4(10): 68-779.
Haag M., Magada O. N., Claassen N., Bohmer L.H. and Kruger M. C. Omega-3 fatty acids modulate ATPases involved in duodenal calcium absorption. Prostaglandins Leukot Essent Fatty Acids. 2003. 6 : 423-429.
Jain P. G., Patil S. D., Haswani N. G., Girase M. V. and Suran S. J. Hypolipidemic activity of Moringa oleifera Lam., Moringaceae, on high fat diet induced hyperlipidemia in albino rats. Braz J Pharmacog. 2010. 20: 969-973.
Babatunde S. O., Indira T. N., Bhatnaga A. S., Radha C., Debnath A. G. and Gopala Krishna S. Quality characteristics and stability of Moringa oleifera seed oil of Indian origin. J Food Sci Technol. 2011. 51(3): 503-510.
Ferretti M., Laura B., Francesco C., Marta B., Paola S., Gianluca C., Zavatti M., Viesti D. V., Zanoli P.and Palumbo, C. Structural and histomorphometric evaluations of ferutinin effects on the uterus of ovariectomized rats during osteoporosis treatment. Life Sci. 2012. 90: 161-168.
Zhang D., Wang Z., Qi W. and Zhao, G. The effects of Cordycep sinensis phytoestrogen on estrogen Deficiency-induced osteoporosis in ovariectomized rats. BMC Complement Altern Med. 2014. 14 : 484.
Meguro S., Higashi K., Hase T., Honda Y., Osuka A., Tokimitsu I. and Itakura H. Solubilization of phytosterols in diacylglycerol versus triacylglycerol improves the serum cholesterol-lowering effect. Eur J Clin Nutr. 2001. 55(7) : 513-517.
Choi Y., Ahn C., Rhee H., Choe M., Kim C. and Kim J. Comparative effects of dietary palm oil, perilla oil and soybean oil on lipid profile in different aged rats fed on hypercholesterolemic diets. Biosci, Biotechnol, Biochem. 1993. 57 : 65-68.
Hassan H. A., EL Wakf A. M. and EL Gharib N. E. Role of phytoestrogenicoils in alleviating osteoporosis associated with ovariectomy in rats. Cytotechnology. 2013. 65(4) : 609-619.
Shuid A. N., Chuan L. H., Mohamed N., Jarin K., Fong Y. S. and Soliman I. N. Recycledpalm oil is better than soy oil in maintaining bone properties in a menopausal syndrome model of ovariectomized rats. Asia J Clin Nutr. 2007. 16 : 393-402.
Boulbaroud S., Mesfioui A., Arfaoui A., Quichou A. and El-Hessni A. Preventive effects of flaxseed and sesame oils on bone loss in ovariectomized rats. Pak J Biol Sci. 2008. 11 : 1696-1701.
Byun J. S. and Lee S. S. Effect of soybeans and sword beans on bone metabolism in a rat model of osteoporosis. Ann Nutri Metab. 2010. 56 : 106-112.
Havill L. M., Hale L. G, Newman D. E., Witte S. M. and Mahaney M. C. Bone ALP and OC reference standards in adult baboons (Papio hamadryas) by sex and age. J Med Primatol. 2006. 35 : 97-105.
Farley S. M., Wergedal J. E., Smith L. C., Lundy M. W., Farley J. R. and Baylink D. J. Fluoride therapy for osteoporosis: Characterization of the skeletal response by serial measurements of serum alkaline phosphatase activity. Metabolism. 1987. 36 : 211-218.
Boulbaroud S. Mesfioui A., Arfaoui A., Ouichou A. and El Hessni A. Preventive Effects of Flaxseed and Sesame Oil on Bone Loss in Ovariectomized Rats. 2008. Pak J Biol Sci. 11(13): 1696-1701.
Elwakf A. M., Hassan H. A. and Gharib N. S. Osteoprotective effect of soybean and sesame oils in ovariectomized rats via estrogen-like mechanism. Cytotechnology. 2014. 66: 335-343.
Chiechi L. M., Secreto G., Vimercati A., Greco P., Venturelli E., Pansini F., Fanelli M., Loizzi, P. and Selvaggi, L. The effects of a soy rich diet on serum lipids: the Menfis randomized trial. Maturitas. 2002. 41: 97-104.
Evans D. B., Bunning R. A. and Russell R. G. The effects of recombinant human interleukin-1 on cellular proliferation and the production of prostaglandin E2, plasminogen activator, osteocalcin and alkaline phosphatase by osteoblast-like cells derived from humanbone. Biochem Biophys Res Commun. 1990. 166: 208-216.
Magda M. E. and Fahmy G. E. Anti-osteoporotic effect of medical herbs and calcium supplementation on ovariectomized rats. 2015. J Basic Appl Zool. 72: 81-88.
Elkomy M. M. and Elsaid F. G. Anti-osteoporotic effect of medical herbs and calcium supplementation on ovariectomized rats. J Basic App Zool. 2013. 72 : 81-88.