Using Nitrogen Fixing Bacteria with Biological Supporting Media for Promoting the Plant Growth
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This research was aimed to isolate of nitrogen fixing bacteria and study on structure of dried water hyacinth stem as a biological supporting media for bacterial immobilization to using them for enhancing the growth of Chinese Kale. (Brassica alboglabra) Six nitrogen fixing bacteria were isolated from soil and designed as RD01, RD02, RD03, RD04, RD05, and RD06. The result showed that RD02 is effective isolate of nitrogen fixing capability and growth characteristic which was the maximum of ammonia-nitrogen in nitrogen-free medium found 6.42 mg NH3-N/L for72 hours of incubation. Colony, morphology and some biochemical characteristics of RD 02 showed that it similar to Azotobacter sp. Inside of the supporting media found a large tube at the center surrounded by microtubules that affected for bacterial cell collecting. 5.4 x 104 CFU/ml. of bacterial cell number were found from a piece of supporting media mixed with inoculum (RD02) which higher than the supporting media without inoculums. (control) Furthermore, the using inoculum of nitrogen fixing bacteria mixed with supporting media was promoted the stem ,s hight and the leaf ,s size of Brassica alboglabra at the second week and seventh week of cultivation respectively which significantly difference with the cultivated soil sample mixed inoculum of nitrogen fixing bacteria and uninoculated soil sample.
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