Effects of flower extract from Nelumbo nucifera on high-K+ & Ca2+-induced colonic contraction in diabetic rats

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Published: Jun 30, 2016
Keywords:
Nelumbo nucifera Hypoglycemic activity Diabetic rats Colonic contraction

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Supasorn Sakuljaitrong
Faculty of Science and Technology, Loei Rajabhat University
Nopparat Buddhakala
Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi

Abstract

Gastrointestinal (GI) disorder has been commonly found in diabetic patients. Diabetes can also affect GI motility through changes in intestinal smooth muscle or alterations in extrinsic neuronal control. The exact mechanism of the intestinal is not fully understood. The aim of this study was therefore to investigate the effect of N. nucifera flower extract (NNFE) on high K+ and Ca2+-ion induced colonic contraction in diabetic rats. Male albino Wistar rats were induced with 65 mg/kg b.w. streptozotocin. All groups of rats were daily once oral administered of NNFE (250 mg/kg b.w.) for 8 weeks. The rats were sacrificed by asphyxiation with CO2. Longitudinal colonic smooth muscle strips of the rats were prepared for SNP-reduced contraction investigation. The results showed that NNFE significantly (p<0.05) reduced the contractile responses of colonic smooth muscle strips of the rats in the presence of K+ and Ca2+-ion. Also, NNFE significantly (p<0.05) reduced the responses of the strips in the presence of SNP. The changes in intracellular Ca2+ concentrations lead to fusion of insulin-containing vesicles to the plasma membrane and subsequent rapid exocytosis of insulin from β cells. The data indicated that in vivo study of the NNFE on hypoglycemic activity, producing 44% reduction in blood glucose levels and inhibited colonic contractile activity in diabetic rats.

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How to Cite
1.
Sakuljaitrong S, Buddhakala N. Effects of flower extract from Nelumbo nucifera on high-K+ & Ca2+-induced colonic contraction in diabetic rats. Prog Appl Sci Tech. [Internet]. 2016 Jun. 30 [cited 2024 May 5];6(1):25-38. Available from: https://ph02.tci-thaijo.org/index.php/past/article/view/243151
Issue
Vol. 6 No. 1 (2016): January - June 2016
Section
Biology and Bioresource technology

References

R. Mathison and J.S. Davison, “Modified smooth muscle responses of jejunum in streptozotocin-diabetic rats”. J. Pharmacol. Exp. Ther. 244 (1988): 1045-1050.

J. Chesta, E.S. Debman, S.K.S. Srai and O. Epstein, “Delayed stomach to caecum transit time in the diabetic rat. Possible role of hyperglucagonaemia”. Gut. 31 (1990): 660-662.

C. Talubmook, “The influence of elevated glucose levels and the diabetic state on neuromuscular function in the gut”. Ph.D. thesis. University of Hertfordshire: UK. 2002.

S.D. Smith, S. Christopher, M.D. Williams, D. Christopher, M.D. Ferris. “Diagnosis and treatment of chronic gastroparesis and chronic intestinal pseudo-obstruction”. Gastroenterology-clinics-of-north. 32 (2003): 619-658.

T. J. Ulahannan, A. Amaratunga. “Successful treatment of diabetic autonomic diarrhea with monthly subcutaneous lanreotide”. Case Report. (2009): 236-328.

A. Shirwaikar, K. Rajendran, C.D. Kumar. “In vitro antioxidant studies of Annona squamosa Linn. Leaves”. J Ethnopharmacol. 91 (2004): 171-175.

J. M. Malins. J. M. Frence, “Diabetic diarrhea”. Q. J. Med, 26. (1957): 467-480.

J. Hanm, I.D. Jung and W. S. Park, Physiological roles of K+ channels in vascular smooth muscle cells. J. Smooth Muscle Res. 44 (2008): 65-81.

C. G. Sobey. Potassium Channel Function in Vascular Disease. Arterioscler Thromb Vasc Biol. 21 (2016): 28-38.

A.P. Somlyo and A.V. Somlyo, “Signal transduction and regulation in smooth muscle”. Nuture. 372 (1994): 231-236.

J.D. Ward. Diabetic neuropathy. Br. Med. Bull. 45 (1976): 111-126.

D.A. Green. Prevention and treatment of neuropathy. In Pharmacology of Diabetes: Present Practice and Future Perspective, ed. By CE Mogensen and E. Stand Berlin: Walter de Gruyter. 13 (1991):297-324.

S. Sakuljaitrong, N. Buddhakala, S. Chomko and C. Talubmook. Effects of Flower Extract from Lotus (Nelumbo nucifera) on Hypoglycemic and Hypolipidemic in Streptozotocin-induced Diabetic Rats. International Journal of Scientific & Engineering Research. 4 (2013): 1441-1446.

P. Trongtorsak, N. Teerakulkittipong, J. Panyajirawut, and N. Athipchartsiri, “Effects of Crude Leaf Extract of Nelumbo nucifera Gaertn. on Blood Pressure in Normotensive and Hypertensive Rats”. Thai J Pharmacol. 29(2) (2007): 1-10.

J. Yin and J.D.Z. Chen, “Gastrointestinal motility disorders and acupuncture”. Autonomic Neuroscience-Basic & Clinical. 157 (2010): 31-37.

P. Manimekalai, C. Davidraj, R. Dhanalakshmi, B. Sudhakar. “Anti-oxidant activity of alcoholic extract of Nelumbo nucifera on streptozotozin induced diabetic albino wistar rats”. Indian J Med & Healthcare. 1(3) (2012): 70-77.

R.Kagyung, P.R. Gajurel, P. Rethy and B. Somgh. Ethnomedicinal plants used for gastro-intestinal diseases by Adi tribes of Dehang-Debang Biosphere Reserve in Arunachal Pradesh. Indian J. Tradit. Knowl.. 9 (3) (2010): 496-501.

G. Shafiee, M. Mohajri-Techrani, M. Pajouhi, B. Larijani, “The importance of hypoglycemia in diabetic patients”. BioMed Central. 11(17) (2012). 1-7,.

J. D. Ward. “Diabetic neuropathy”. Br.Med. Bull. 45 (1989): 111-126.

Q. Sun, N. Sekar, I. Goldwaser, E. Genshonov, M. Fridkin, Y. Shechter. “Vanadate restores glucose 6 phosphate in diabetic rats: a mechanism to enhance glucose metabolism”. Am J Physiol Endocrinol Metab. 279(2) (2000): E403-410.

S. Kupittayanant, M.J. Luckas, S. Wray. “Effect of inhibiting the sarcoplasmic reticulum on spontaneous and oxytocin-induced contractions of human myometrium”. International Journal of Obstetrics & Gynaecology. 109(3) (2002): 289-296.

N. Buddhakala, C. Talubmook, P. Sriyotha, S. Wray, S. Kuppittayanant. “Inhibitory effects of ginger oil on spontaneous and PGF2α-induced contraction of Rat Myometrium. Planta Med. 74 (2008): 385-391.

D.A. Green. “Prevention and treatment of neuropathy. In Pharmacology of Diabetes”. Present Practice and Future Perspective, ed. By CE Mogensen and E.Stand Water de Gruyter, Berlin. 1, 3. 1991. 297-324.

J.C. Huralikuppi, A.B. Christopher, P.M. Stephen, “Antidiabetic effect of Nelumbo nucifera (Gaertn.) extract: part II,” Phytother Res. 5 (1991): 217-223.

P.K. Mukherjee, K. Saha, B.M. Pal, B.P. Saha. “Studies on sychopharmaco-logical effects of Nelumbo nucifera Gaertn. rhizome extrzct”. Journal of Ethnopar-macology 54 pp. (1996): 63-67.

C.M. Florkowski, B. R.Rowe, S. Wightingale, T.C. Hawey, and A.H. Barnett. “Clinical and neurophysiological studies of aldose reductase inhibitor ponalrestal in chronic symptomatic diabetic peripheral neuropathy”. Diabetes. 40 (1991): 129-133.

A. Forrest, J.D. Huizinga, X.Y. Wang, L.W. Liu, M. Parsons. “Increase in stretch-induced rhythmic motor activity in the diabetic rat colon is associated with loss of ICC of the submuscular plexus”. Am J. Physiol. Gastrointest. Liver Physiol. 294 (2008): G315-G326.

S. Hemalatha, A.K. Wahi, P.N. Singh, J.P.N. Chansouria. “Hypoglycemic activity of Withania Coagulans Dunal in streptozotocin induced diabetic rats”. Journal of Ethnopharmacology. 93 (2004): 261-264.

C. Talubmook, “Effect of polysaccharide from Phellinus ignarius (L.) Quel. on hematological values and blood cell characteristics in diabetic rats”. Journal of the Microscopy Society of Thailand. 22 (2008): 42-45.

C. Talubmook, A. Forest M. Parsons, “Strepto-zotocin-induced diabetes modulates presynaptic and postsynaptic function in the rat ileum”. European Journal Pharmacology. 469 (2003): 153-158.

A. Forrest, A. Mollenman, d M. Parsons. “The responses to manipulation of extracellular and intracellular calcium are altered in the streptozotocin-diabetic rat colon and ileum”. European Journal of Pharmacology. 500 (2003): 77- 83.

T. Nelson and J.M. Quayle. “Physiological role and properties of potassium channels in arterial smooth muscle”. Am J Physiol. 268 (1995): C799-822.

M.J. Taggart, S. Wray. “Contribution of sarcoplasmic reticulum to smooth muscle contraction activation: gestational dependence in isolated rat uterus”. J Physiol. 511 (1998): 133-144.

H. Zhou, D.H. Kong, Q.W. Pan, H.H. Wang. “Sources of calcium in agonist-induced contraction of rat distal colon smooth muscle in vitro”. World J Gastroenterol. 14(7) (2008): 1077-1083.

A.I. Patel, J.K. Hennan and J. Dianond, “Activation of Gunanosine 3, 5-Cyclic Monophosphate (cGMP)-Dependent Protein Kinase in Rat Vas Deferens and Distal Colon is Not Accompanied by Inhibition of Contraction”. JPET. 283 (1997): 894-900.

P. Trongtorsak, N. Chaichit and J. Panyjirawu. “Acute and chronic hypotensive effects of crude leaf extract of Nelumbo nucifera Gaertn”. Thai Journal of Pharma-ceutical Sciences. 17 (2004): 68-69.

J. Yin and J.D.Z. Chen, “Gastrointestinal motility disorders and acupuncture”. Autonomic Neuroscience-Basic & Clinical. 157 (2010) 31-37.

A. Forrest, M. Parsons. “The enhanced spontaneous activity of the diabetic colon is not the consequence of impaired inhibitory control mechanisms”. Auton Autacoid Pharmacol. 23(3) (2003): 149-158.

S. Wray, “Uterine contraction and physiological mechanism of modulation”. Am J Physiol. 264 (1993): C1-C18.

S. Shabir, L. Borisova, S. Wray, T. Burdyga. “Rho-kinase inhibition and electromechanical coupling in rat and guinea-pig ureter smooth muscle: Ca2+-dependent and independent mechanisms”. J Physiol. 560(3) (2004): 839-855.

P.F. Dillon, M.O. Aksoy, S.P. Driska, and R.A. Murphy, “Myosin phosphorylation and the cross-bridge cycle in arterial smooth muscle,” Science. 211 (1981): 495-497, 1981.