Biomass and lipid content of Scenedesmus acutus UD01 cultured in wastewater effluent

Main Article Content

Sorrachat Thiamdao
Thanitsara Inthasotti

Abstract

The cultivation of microalgae as an alternative energy source has increasingly become popular in various  parts of  the  world. However,  the high cost of the media used in cultivation has directly affected the  manufacturing  cost.  In  this  study,  we have  aimed  to  culture  Scenedesmus acutus UD01  in  the effluent acquired from the  biogas  system  of  a piggery  farm (BGF),  the wastewater of a sugar  factory (SF),  and  modified  JM(MJM).  After  7  days  of  cultivation  under  white  fluorescence  at  room temperature,  the  highest  growth  rate  was  found  in  BGF  and  the  second highest  was recorded in  MJM.  In the  study on the  lipid contents,  the  results  revealed  that  20.09±0.18% of lipid  was  obtained  from  S. acutus UD01 cultured  in  SF, while 17.72±1.05% of lipid was recorded in BGF. In considering the lipid composition and the process of culturing conducted in the low-cost media, there is a desire to find an achievable  economic procedure  for  algal  biofuel  production.

Article Details

How to Cite
1.
Thiamdao S, Inthasotti T. Biomass and lipid content of Scenedesmus acutus UD01 cultured in wastewater effluent. Prog Appl Sci Tech. [Internet]. 2016 May 27 [cited 2024 Nov. 7];6(1):48-57. Available from: https://ph02.tci-thaijo.org/index.php/past/article/view/243153
Section
Biology and Bioresource technology

References

L.F. Wu, P.C. Chen, C.M. Lee. The effects of nitrogen sources and temperature on cell growth and lipid accumulation of microalgae. Int Biodeterior Biodegrad. 85 (2013): 506- 510.

Y. Chisti. Biodiesel from microalgae 5 beats bioethanol. Trends Biotechnol. 26(3)(2008): 126–131.

M.E. Huntley, D.G. Redalje. CO2 mitigation and renewable oil from photosynthetic microbes: a new appraisal. Mitigat Adapt Strat Global Change. 12(4)(2007): 573–608.

F.G. Avién, J.M. Fernández, J.J. Magán, E. Molina. Production cost of a real microalgae production plant and strategies to reduce it. Biotechnol Adv. 30(2012): 1344–1353.

J.K. Pittman, A.P. Dean, O. Osundeko. The potential of sustainable algal biofuel production using wastewater resources. Bioresour Technol. 102(2011): 17-25.

Y. Chisti. Biodiesel from microalgae. Biotechnol Adv. 25(3)(2007): 294–306.

B.G. Subhadra. Water management policies for the algal biofuel sector in the Southwestern United States. Appl Energy. 88(10)(2011): 3492–3498.

S. Chinnasamy, A. Bhatnagar, R.W. Hunt, K.C. Das. Microalgae cultivation in a wastewater dominated by carpet mill effluents for biofuel applications. Bioresour Technol. 101(2010): 3097– 3105.

L. Jiang, J. Wang, S. Liang, X. Wang, P. Cen, Z. Xu. Butyric acid fermentation in a fibrous bed bioreactor with immobilized Clostridium tyrobutyricum from cane molasses. Bioresour Technol. 100(2009): 3403–3409.

W.J. Oswald, H.B. Gotaas, C.G. Golueke, E.R. Kellen. Algae in waste treatment. Sewage Ind Waste. 29(1957): 349-350.

F.D. Caprio, P. Altimari, F. Pagnanelli. Integrated biomass production and biodegradation of olive mill wastewater by cultivation of Scenedesmus sp. Algal Research. 9(2015): 306–311.

A. Bhatnagar, S. Chinnasamy, M. Singh, K.C. Das. Renewable biomass production by mixotrophic algae in the presence of various carbon sources and wastewaters. Appl Energ. 88(2011): 3425–3431.

H. Wang, H. Xiong, Z. Hui, X. Zeng. Mixotrophic cultivation of Chlorella pyenoidosa with dilutedprimary piggery wastewater to producelipids. Bioresour Technol. 104(2012): 215-220.

S. Canovas, B. Picot, C. Casellas, H. Zulkifi, A. Dubois, J. Bontoux. Seasonal development of phytoplankton and zooplankton in a high-rate algal pond. Water Sci Technol. 33(1996): 199-206.

O. Pulz, W. Gross. Valuable products from biotechnology of microalgae. Appl Microbiol Biotechnol. 65(6)(2004): 635–648.

J.I. Park, H.C. Woo, J.W. Lee. Production of bio-energy from microalgae: status and perspectives. Kor J Chem Eng. 46(5)(2008): 833–844.

T. Matsunaga, M. Matsumoto, Y. Maeda, H. Sugiyama, R. Sato, and T. Tanaka. Characterization of marine microalga, Scenedesmus sp. strain JPCC GA0024 toward biofuel production. Biotechnol Lett. 31( 9)(2009): 1367–1372.

M.K. Ji, H.S. Yun, S. Park, H. Lee, Y.T. Park, S. Bae, J. Ham, J. Choi. Effect of food wastewater on biomass production by a green microalga Scenedesmus obliquus for bioenergy generation. Bioresour Technol. 179(2015): 624-628.

M.K. Ji, H.S. Yun, S. Park, H. Lee, Y.T. Park, S. Bae, J. Ham, J. Choi. Mixotrophic cultivation of a microalga Scenedesmus obliquus in municipal wastewater supplemented with food wastewater and flue gas CO2 for biomass production. J Environ Manag. 159(2015): 115-120.

D.Y. Shin, H.U. Cho, J.C. Utomo, Y.N. Choi, X. Xu, J.M. Park. Biodiesel production from Scenedesmus bijuga grown in anaerobically digested food wastewater effluent. Bioresour Technol. 184(2015): 215-221.

A.S. Thompson, J.C. Rhodes, I. Pettman. Natural research council culture collection of algae and protozoa-catalogue of strains. Toxicon. 41(1988): 979-988.

E.G. Bligh, W.E. Dyer. A rapid method of total lipid extraction and pureification. Can J Biochem Physiol. 37(8)(1959): 911-917.

M.K. Kim, J.W. Park, C.S. Park, S.J. Kim, K.H. Jeun, M.U. Chang, J. Acreman. Enhanced production of Scenedesmus spp. (green microalgae) using a new medium containing fermented swine wastewater. Bioresour Technol. 98(2007): 2220-2228.

V. Ashokkumar, Z. Salam, O.N. Tiwari, S. Chinnasamy, S. Mohammed, F. Nasir. An integrated approach for biodiesel and bioethanol production from Scenedesmus bijugatus cultivated in a vertical tubular photobioreactor. Energ Convers Manag. 101(2015): 778-786.

S.H. Ho, S.W. Huang, C.Y. Chen, T. Hasunuma, A. Kondo, J.S. Chang. Bioethanol production using carbohydrate-rich microalgae biomass as feedstock. Bioresour Technol. 135(2013): 191–198.

J. Anand, M. Arumugam. Enhanced lipid accumulation and biomass yield of Scenedesmus quadricauda under nitrogen starved condition. Bioresour Technol. 188(2015): 190-194.

M.C. Ceron Garcia, F.G. Camacho, A.S. Miron, J.M.F. Sevilla, Y. Chisti, E.M. Grima. Mixotrophic production of marine microalga Phaeodactylum tricornutum on various carbon sources. J Microbiol Biotechnol. 16 (5)(2006): 689-694.

M.C.C . Garci, J.M.F. Sevilla, F.G.A. Fernandez, E.M. Grima, F.G. Camacho. Mixotrophic growth of Phaeodactylum tricornutum on glycerol: growth rate and fatty acid profile. J Appl Phycol. 12(3-5)(2000): 239-248.

S. Sanchez, M.E. Martinez, M.T. Espejo, R. Pacheco, F. Espinola, G. Hodaifa. Mixotrophic culture of Chlorella pyrenoidosa with olive-mill wastewater as the nutrient medium. J Appl Phycol. 13(5)(2001): 443-449.