Development of hydrogen peroxide biosensor for detection of hydrogen peroxide concentration in milk
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Abstract
An analytical method was developed for detection of hydrogen peroxide concentration in milk using biosensor technique. Peroxidase enzyme and a spectrophotometer were used as biological sensing element and detector, respectively. The principle of this method is based on the oxidation reaction of hydrogen peroxide by peroxidase enzyme. The oxidation reaction of hydrogen peroxide and o-dianisidine were catalyzed by peroxidase enzyme, which results in brown solution. Under acidic condition, the color of solution turns from brown to pink. The absorptions were detected with spectrophotometer at the wavelength of 540 nm. The results were found that the absorbance were proportional to the concentrations of hydrogen peroxide. Under optimum conditions, the calibration curves were linear in the range of concentrations 0.4-3.0 ppm with the analysis time of 5 min. The advantages of a developed biosensor were simple, short analysis time, high sensitivity and accuracy method.
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