Screening of 5'-Phosphodiesterase Producing Bacteria from Raw Fish Sauce
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Abstract
Twenty pure bacterial cultures isolated from raw fish sauce were selected for 5´- phosphodiesterase (5´- PDE) producing ability on agar medium and liquid medium containing 0-3.0 M NaCl. The results showed that isolate AB 005 could produce the highest 5´- PDE activity. The selected isolate was further identified on the basis of morphological, biochemical and compared 16S rDNA gene to other bacteria. It was concluded that isolate AB 005 belonged to Bacillus pumilus and classified as halotolerant bacteria. A halotolerant Bacillus pumilus AB 005 could produce the highest 5´- PDE activity at pH of 7.0 and 37 oC in liquid medium without NaCl. Growth and production of 5´- PDE from Bacillus pumilus AB 005 in 5.0 L fermenter using liquid medium was investigated. A typical pattern of 5´- PDE production showed that enzyme production was coupled to cell multiplication and the maximum 5´- PDE production of 10.50 unit/ml was observed at 36 hr.
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References
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