The Comparison of Antioxidant Activity and Acetylcholinesterase Inhibitory Activity of Unrefined Black Rice and Parboiled Germinated Unrefined Black Rice Extracts of Thai Local Rice (Mae Phaya Tong Dum)

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Pimjai Suwannawong
Duangkamol Phromnoi
Watcharee Waratchareeyakul
Nuntaporn Moonrungsee


Mae Phaya Tong Dum rice is the local black rice of Chanthaburi province, Thailand. This rice has the purple-black pigments presented in the rice bran. Black rice has reported that there is a good source of several bioactive compounds. This research emphasized the comparing of the antioxidant and anti-acetyl cholinesterase activities of unrefined black rice (UBR) and parboiled germinated unrefined black rice (PGUBR) extracts of Mae Phaya Tong Dum rice. The results found that UBR extracts obtained higher antioxidant and enzyme inhibitory activities than that of PGUBR extracts. The methanol extracted of UBR showed the highest antioxidant activity (IC50 = 217 µg/ml) and total phenolic content (469.716 µgGAE/g) more than that of methanol extracts of PGUBR (IC50 = 534 µg/ml and 334.489 µgGAE/g, respectively). Whereas hexane extracts were the best enzyme inhibitor, UBR had higher inhibition than PGUBR (IC50 = 58 and 181 µg/ml respectively). Using heat in the process of PGUBR from the Mae Phaya Thong Dam variety resulted in a decrease in the bioactivity of the black rice seed extract. Therefore, in the process of Germinated hang rice, it should be considered the heat used.


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Suwannawong P, Phromnoi D, Waratchareeyakul W, Moonrungsee N. The Comparison of Antioxidant Activity and Acetylcholinesterase Inhibitory Activity of Unrefined Black Rice and Parboiled Germinated Unrefined Black Rice Extracts of Thai Local Rice (Mae Phaya Tong Dum). Prog Appl Sci Tech. [Internet]. 2021 Jul. 13 [cited 2022 May 29];11(2):37-41. Available from:
Pure and Applied Chemistry


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