Development of thermosensitive chitosan/collagen hydrogel with antibacterial properties using biosynthesized silver nanoparticles from aqueous extract of fingerroot for bone grafting
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Abstract
Thermosensitive hydrogel has been developed and used in tissue engineering to promote bone regeneration and deliver active substances. This study aims to prepare and characterize chitosan/collagen hydrogel with antibacterial properties by incorporating biosynthesized silver nanoparticles (AgNP). The aqueous extract of fingerroot (Boesenbergia rotunda) was used as a reducing agent for AgNP synthesis. Thus, the AgNPs were characterized by spectrophotometry, morphological, and biological testing. The AgNPs were spherical with diameter ranges from 20-40 nm with inhibitory activity of Staphylococcus aureus and Pseudomonas aeruginosa. The synthesized AgNPs were mixed with chitosan/collagen hydrogel with concentrations of 1 % (w/v). The thermosensitive chitosan/collagen hydrogel filled with synthesized AgNP was investigated with rheological, structural, chemical, and biological properties. The results demonstrated that adding AgNP affects gelation temperature, gelation time, and hydrogel modulus. The structural morphology (size and porosity) of hydrogel after the gelation process was changed with loading synthesized AgNP. Furthermore, the AgNP-filled hydrogels exhibited more excellent antibacterial performance than the unfilled hydrogel.
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เนื้อหาและข้อมูลในบทความที่ลงตีพิมพ์ในวารสารวารสารวิทยาศาสตร์และเทคโนโลยีถือเป็นข้อคิดเห็นและความรับผิดชอบของผู้เขียนบทความโดยตรงซึ่งกองบรรณาธิการวารสาร ไม่จำเป็นต้องเห็นด้วย หรือร่วมรับผิดชอบใด ๆ
บทความ ข้อมูล เนื้อหา รูปภาพ ฯลฯ ที่ได้รับการตีพิมพ์ในวารสารวารสารวิทยาศาสตร์และเทคโนโลยีถือเป็นลิขสิทธิ์ของวารสารวารสารวิทยาศาสตร์และเทคโนโลยีหากบุคคลหรือหน่วยงานใดต้องการนำทั้งหมดหรือส่วนหนึ่งส่วนใดไปเผยแพร่ต่อหรือเพื่อกระทำการใด ๆ จะต้องได้รับอนุญาตเป็นลายลักษณ์อักษรจากวารสารวารสารวิทยาศาสตร์และเทคโนโลยี ก่อนเท่านั้น
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