Chemical constituents and cytotoxic effects of the essential oil from the root of Cyperus kyllingia Endl.

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Noppawan Intecha
Natcha Injan
Wanrudee Keawmesri
Manat Jaimasith
Chayanan Jitmanee
Sorachai Khamsan


The chemical constituents of the essential oil from the root of Cyperus kyllingia Endl. were obtained from hydrodistillation and subsequently analyzed by a gas chromatography/mass spectrometry (GC-MS) technique. Twenty-eight compounds were identified, accounting for 89.62% of the total oil that consisted mainly of oxygenated sesquiterpenes (50.21%) and sesquiterpene hydrocarbons (38.08%). α-Cadinol (18.62%), caryophyllene oxide (12.18%), α-muurolol (11.56%), Cyperene (10.15%), and α-Cyperone (5.72%) were identified as the major components. The essential oil showed significantl cytotoxicity against NCI-H187, and MCF-7 cell lines with IC50 values of 6.7 and 13.3 µg/mL, and was non-cytotoxic against Vero cells. Furthermore, the oil was active against Mycobacterium tuberculosis with a MIC value of 25.0 µg/ mL.

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