Detection of Oxacillinase Genes in Acinetobacterbaumannii Isolated from the Maharaj Nakhon Si Thammarat Hospital

Abstract

This study aims to detect oxacillinase genes in 68 isolates of Acinetobacter baumannii obtained from clinicalisolates in Maharaj Nakhon Si Thammarat  Hospital. The minimal inhibitory concentration (MIC) of imipenem and meropenem for all isolates were determined by an agar dilution method. All isolates were resistant to imipenem and meropenem. The MIC range of imipenem and meropenem was 16 to 64 and 16 to ≥128 μg/ml, respectively. The presence of blaOXA-23-like, blaOXA-24-like, blaOXA-51-like และ blaOXA-58-like genes was explored using multiplex-PCR amplification. The results showed that all isolates (100%) harbored blaOXA-23 gene which is frequently found in carbapenem-resistant A. baumannii. These findings are needed for hospital staff to find measures for surveillance, control and prevention of antimicrobial agent resistant bacteria in hospital.