Effect of Synthetic ChalconeDerivatives on Growth Inhibition and Apoptosis Inductionin Breast Cancer Cell Line (SKBR-3)

Abstract

This study aims to examine the effects of synthetic chalcone derivatives including 4-methoxychalcone and 4-chlorochalcone. Their anti-proliferative and anti-apoptotic effects on breast cancer cell line SKBR-3 were investigated by MTT and DAPI staining, respectively. Results from MTT assay indicated that 4-methoxychalcone and 4-chlorochalcone could inhibit the proliferation of SKBR-3 cell in a dose dependent manner with IC50 doses of 18.35 g/ ml and 15.93 g/ ml, respectively. Incubation of SKBR-3 cell with IC50 doses of both synthetic chalcone derivatives for 24 hours was found increasing apoptosis of this cell. In addition, nuclear condensation could be visualized by DAPI staining. These preliminary results suggest that two types of synthetic chalcone derivatives could be candidate compounds for further investigations of anti-breast cancer agent.