Application of PetrifilmTM Aerobic Count Plate Associated with de Mann Rogasa Sharpe (MRS) Broth for Enumeration Lactic Acid Bacteria
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Abstract
This study was aimed to compare the enumeration of Lactic Acid Bacteria (LAB) between spread plate
method and using of PetrifilmTM Aerobic Count Plate associated with de Mann Rogasa Sharpe (MRS) broth. They were used to enumerate of 4 tested LABs [Pediococcus pentosaceus 1 L14/1 (L14/1), Ped. acidilactici L25 (L25), Lactococcus lactis ssp. lactis 1 L26 (L26), Enterococcus faecium N15 (N15)] and LABs from fermented milks. The results indicated that both methods could be enumerated the tested LAB from starter (1.5×108 cfu/ml) at dilution rate of 10-6 are not statistically different (p < 0.05) but are statistically different when enumerated from yoghurts at dilution rate of 10-6 and 10-8. However, the detection limit of colonies of LAB could be enumerated, using PetrifilmTM Aerobic Count Plate associated with MRS broth, at dilution rate of 10-8. From the results it may imply that PetrifilmTM Aerobic Count Plate associated with MRS broth could be efficaciously used for enumeration
of LABs.
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