Efficacy of Essential Oils from Clove (Syzygium aromaticum) and Cassumunar Ginger (Zingiber montanum) as Anesthetic Agents for Fruit Fly (Drosophila melanogaster)

Abstract

Efficacy of essential oils from clove (Syzygium aromaticum) and cassumunar ginger (Zingiber montanum) 100 % intensity as anesthetic agents to fruit fly (Drosophila melanogaster) in genetic laboratory compared with diethyl ether. The results revealed that fruit flies were better anesthetized by cassumunar ginger essential oil, compared to clove essential oil. Fifty percent and 100 percent of flies can be anesthetized by cassumunar ginger essential oil within 5 and 10 minutes respectively. Some flies started recovery at 30 minutes then all of them recovered after 55 minutes while clove essential oil anesthetized all flies within 25 minutes and recovery time started at 30 minutes. However, anesthetic efficacy investigation showed that diethyl ether exhibited better anesthetic property, compared to both herb essential oils. All flies were anesthetized by diethyl ether within 3 minutes and recovered within 10-35 minutes. This study suggested that cassumunar ginger essential oil can be used as an anesthetic agent instead of diethyl ether in genetic laboratory.